Summary
It has been demonstrated that the lack of epithelium in healthy airways from different species modifies the reactivity of tracheobronchial smooth muscle. In the present work we have investigated the effect of airway epithelium removal in tracheal chains from guinea pigs sensitized to ovalbumin by inhalatory route and unsensitized animals. We found that in both groups the mechanical removal of airway epithelium produced an increase in the tracheal sensitivity and maximum contractile response to histamine. The greatest responses were observed in epithelium-lacking sensitized tracheas. These findings, at least in vitro, support the idea that the damage of respiratory epithelium plays an important role in modulating the airway reactivity and this is more noteworthy in tracheal chains from previously sensitized guinea pigs.
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References
Barnes PJ, Cuss FM, Palmer JP (1985) The effect of airway epithelium on smooth muscle contractility in bovine trachea. Br J Pharmacol 86:685–691
Cockcroft DW, Killian DN, Mellon JJA, Hargreave FE (1977) Bronchial reactivity to inhaled histamine: A method and clinical survey. Clin Allergy 7:235–243
Flavahan NA, Aarhus LL, Rimele TJ, Vanhoutte PM (1985) Respiratory epithelium inhibits bronchial smooth muscle tone. J Appl Physiol 58:834–838
Foster RW (1960) Paired tracheal chain preparation. J Pharm Pharmacol 12:189–191
Hay DWP, Farmer SG, Raeburn D, Robinson VA, Fleming WW, Fedan JS (1986) The reactivity of guinea-pig respiratory smooth muscle. Eur J Pharmacol 129:11–18
Hay DWP, Raeburn D, Farmer SG, Fleming WW, Fedan JS (1986) Epithelium modulates the reactivity of ovalbumin-sensitized guinea-pig airway smooth muscle. Life Sci 38:2461–2468
Hogg JC, Eggleston PA (1984) Is asthma an epithelial disease? Am Rev Respir Dis 129:207–208
Holroyde MC (1986) The influence of epithelium on the responsiveness of guinea-pig isolated trachea. Br J Pharmacol 87:501–507
Hulbert WC, Walker DC, Jackson A, Hogg JC (1981) Airway permeability to horseradish peroxidase in guinea pigs: The repair phase after injury by cigarette smoke. Am Rev Respir Dis 123:320–326
Ilhan M, Sahin I (1986) Tracheal epithelium releases a vascular smooth muscle relaxant factor: Demonstration by bioassay. Eur J Pharmacol 131:293–296
Laitinen LA, Heino M, Laitinen A, Kava F, Haahtela T (1985) Damage of the airway epithelium and bronchial reactivity in patients with asthma. Am Rev Respir Dis 131:599–607
Murlas CG, Roum JH (1985) Sequence of pathologic changes in the airway mucosa of guinea pigs during ozone-induced bronchial hyperreactivity. Am Rev Respir Dis 131:314–320
Nadel JA (1983) Bronchial reactivity. Adv Intern Med 28:207–223
Reaburn D, Hay DWP, Robinson VA, Farmer SG, Fleming WW, Fedan JS (1986) The effect of verapamil is reduced in isolated airway smooth muscle preparations lacking the epithelium. Life Sci 38:809–816
Snedecor GW, Cochran WG (1967) Statistical methods, 6th edn. Iowa State University Press, Ames, Iowa
Tschirhart E, Landry Y (1986) Airway epithelium releases a relaxant factor: Demonstration with substance P. Eur J Pharmacol 132:103–104
Vargas MH, Montaño LM, Páramo I, Selman ME (1987) An inhalatory method using ovalbumin andBordetella pertussis for inducing allergic bronchoconstriction in guinea pigs. Med Sci Res 15:179–180
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Montaño, L.M., Selman, M., Ponce-Monter, H. et al. Role of airway epithelium on the reactivity of smooth muscle from guinea pigs sensitized to ovalbumin by inhalatory method. Res. Exp. Med. 188, 167–173 (1988). https://doi.org/10.1007/BF01852317
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DOI: https://doi.org/10.1007/BF01852317