Research in Experimental Medicine

, Volume 188, Issue 2, pp 123–129 | Cite as

When should treatment of acute experimental pancreatitis be started?

The Early Phase of Bile-Induced Acute Pancreatitis
  • P. G. Lankisch
  • U. Pohl
  • J. Otto
  • G. Rahlf
Original Papers


Sodium taurocholate pancreatitis in the rat is a frequently used experimental model for evaluating therapeutical regimes in this disease. It is, however, uncertain when treatment should be started, as the early phase of this experimental model and thus the time when the pancreatitis really develops is unknown. Serum and pancreatic enzymes, as well as pancreatic morphology, were therefore studied 5, 30, and 60min after induction of sodium taurocholate pancreatitis. It was found that increase in serum enzymes and decrease in pancreatic enzymes and morphological changes characteristic for acute pancreatitis develop as early as 5 and 30 min after induction of pancreatitis. Thus, therapy in this model may be started shortly after induction of acute pancreatitis.

Key words

Acute experimental pancreatitis Sodium taurocholate Pancreatic enzymes Pancreatic morphology Rat 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Adler G, Hupp T, Kern HF (1979) Course and spontaneous regression of acute pancreatitis in the rat. Virchows Arch [Pathol Anat] 382:31–47Google Scholar
  2. 2.
    Aho HJ, Nevalainen TJ (1980) Experimental pancreatitis in the rat. Ultrastructure of sodium taurocholate-induced pancreatic lesions. Scand J Gastroenterol 15:417–424PubMedGoogle Scholar
  3. 3.
    Aho HJ, Nevalainen TJ, Aho AJ (1983) Experimental pancreatitis in the rat. Development of pancreatic necrosis, ischemia, and edema after intraductal sodium taurocholate injection. Eur Surg Res 15:28–36PubMedGoogle Scholar
  4. 4.
    Blamey SL, Imrie CW, O'Neill J, Gilmour WH, Carter DC (1984) Prognostic factors in acute pancreatitis. Gut 25:1340–1346PubMedGoogle Scholar
  5. 5.
    Bockman DE, Schiller WR, Suriyapa C, Mutchler JHW, Anderson MC (1973) Fine structure of early experimental acute pancreatitis in dogs. Lab Invest 28:584–592PubMedGoogle Scholar
  6. 6.
    Brackett KA, Crocket A, Joffe SN (1983) Ultrastructure of early development of acute pancreatitis in the rat. Dig Dis Sci 28:74–84PubMedGoogle Scholar
  7. 7.
    Buschmann-Kaspari H (1986) Diagnostische Wertigkeit von Pankreasenzymentgleisungen bei akuter Pankreatitis — einschließlich Methodenvergleich — und Korrelation der Enzymverläufe mit dem klinischen Krankheitsverlauf. Dissertation, Med Fak Univ GöttingenGoogle Scholar
  8. 8.
    Dickson AP, Foulis AK, Imrie CW (1986) Histology and bacteriology of closed duodenal loop models of experimental acute pancreatitis in the rat. Digestion 34:15–21PubMedGoogle Scholar
  9. 9.
    Erlanger BF, Kokowsky N, Cohen W (1961) The preparation and properties of two new chromogenic substrates of trypsin. Arch Biochem Biophys 95:271–278PubMedGoogle Scholar
  10. 10.
    Ferrie MM, O'Hare R, Joffe SN (1978) Acute and chronic pancreatitis in the rat caused by a closed duodenal loop. Digestion 18:280–285PubMedGoogle Scholar
  11. 11.
    Freeny PC, Lawson TL (1982) Radiology of the pancreas. Springer, Berlin Heidelberg New YorkGoogle Scholar
  12. 12.
    Gamklou R, Edlund Y (1966) Ductal factors in the pathogenesis of acute pancreatitis in the rat. Scand J Gastroenterol 1:94–100PubMedGoogle Scholar
  13. 13.
    Heinkel K (1953) Die Ratte als Versuchstier in der experimentellen Pankreasdiagnostik. II. Mitteilung. Die Erzeugung einer akuten hämorrhagischen Pankreatitis durch Injektion von Gallensäure in den Ductus pancreaticus. Klin Wochenschr 31:815PubMedGoogle Scholar
  14. 14.
    Horn J, Heymer B, Merkle N, Meyer H, Diebolder H (1980) Die duktale Obstruktion als pathogenetischer Faktor der akuten Pankreatitis. Eine tierexperimentelle Untersuchung. Z Gastroenterol 18:98–106PubMedGoogle Scholar
  15. 15.
    Lankisch PG (1984) Acute and chronic pancreatitis. An update on management. Drugs 28:554–564PubMedGoogle Scholar
  16. 16.
    Lankisch PG, Ihse I (1987) Bile-induced acute experimental pancreatitis. Scand J Gastroenterol 22:257–260PubMedGoogle Scholar
  17. 17.
    Lankisch PG, Winckler K, Bokermann M, Schmidt H, Creutzfeldt W (1974) The influence of glucagon on acute experimental pancreatitis in the rat. Scand J Gastroenterol 9:725–729PubMedGoogle Scholar
  18. 18.
    Lankisch PG, Koop H, Winckler K, Kunze H, Vogt W (1978) Indomethacin treatment of acute experimental pancreatitis in the rat. Scand J Gastroenterol 13:629–633Google Scholar
  19. 19.
    Lankisch PG, Koop H, Winckler K, Schmidt H (1979) Continuous peritoneal dialysis as treatment of acute experimental pancreatitis in the rat. I. Effect on length and rate of survival. Dig Dis Sci 24:111–116PubMedGoogle Scholar
  20. 20.
    Lankisch PG, Pohl U, Otto J, Göke B (1985) Therapeutic effects of camostate (FOY 305) in acute experimental pancreatitis. Dig Dis Sci 30:979Google Scholar
  21. 21.
    Lombardi B, Estes LW, Longnecker DS (1975) Acute hemorrhagic pancreatitis (massive necrosis) with fat necrosis induced in mice by DL-ethionine fed with a choline-deficient diet. Am J Pathol 79:465–480PubMedGoogle Scholar
  22. 22.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the foline phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  23. 23.
    Maclean N (1977) The role of the surviving pancreas in late fatalities of acute pancreatitis. Br J Surg 64:345–346PubMedGoogle Scholar
  24. 24.
    Murphy D, Imrie CW, Pack A, Davidson JF, Blumgart LH (1976) The mechanism of acute respiratory insufficiency in acute pancreatitis. Br J Surg 63:669Google Scholar
  25. 25.
    Nevalainen TJ, Seppä A (1975) Acute pancreatitis caused by closed duodenal loop in the rat. Scand J Gastroenterol 10:521–527PubMedGoogle Scholar
  26. 26.
    Pfeffer RB, Stasior O, Hinton JW (1957) The clinical picture of the sequential development of acute hemorrhagic pancreatitis in the dog. Surg Forum 8:248–251PubMedGoogle Scholar
  27. 27.
    Popper HL, Necheles H, Russell KC (1948) Transition of pancreatic edema into pancreatic necrosis. Surg Gynecol Obstet 87:79–82Google Scholar
  28. 28.
    Ranson JHC, Rifkind KM, Roses DF, Fink SD, Eng K, Localio SA (1974) Objective early identification of severe acute pancreatitis. Am J Gastroenterol 61:443–451PubMedGoogle Scholar
  29. 29.
    Rao SS, Watt IA, Donaldson LA, Crocket A, Joffe SN (1981) A serial histologic study of the development and progression of acute pancreatitis in the rat. Am J Pathol 103:39–46PubMedGoogle Scholar
  30. 30.
    Rick W (1969) Kinetischer Test zur Bestimmung der Serumlipaseaktivität. Z Klin Chem Klin Biochem 7:530–539Google Scholar
  31. 31.
    Rick W, Stegbauer HP (1970) α-Amylase. Messung der reduzierenden Gruppen. In: Bergmeyer HU (Hrsg) Methoden der enzymatischen Analyse, Bd 1, 2. Aufl. Verlag Chemie, Weinheim/BergstraßeGoogle Scholar
  32. 32.
    Seelig R, Lankisch PG, Koop H, Winckler K, Kaboth U, Seelig HP (1978) Complement system in sodium taurocholate pancreatitis in the rat. Res Exp Med 174:57–65Google Scholar
  33. 33.
    Wanke M (1970) Experimental acute pancreatitis. Curr Top Pathol 52:64–142PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • P. G. Lankisch
    • 1
    • 2
  • U. Pohl
    • 1
    • 2
  • J. Otto
    • 1
    • 2
  • G. Rahlf
    • 1
    • 2
  1. 1.Division of Gastroenterology and Endocrinology, Dept. of Internal MedicineUniversity of GöttingenGöttingenFederal Republic of Germany
  2. 2.Division of General Pathology and Pathologic Anatomy, Dept. of PathologyUniversity of GöttingenGöttingenFederal Republic of Germany

Personalised recommendations