Advertisement

Research in Experimental Medicine

, Volume 180, Issue 1, pp 25–30 | Cite as

The nature of allorosette-forming cells in rats

  • K. W. Lempfrid
  • G. Hermann
Original Contributions

Summary

ARFC appear in the spleen after transplantation of organs or tissues and immunization with nucleated cells between isohistogenic rat strains. The allorosette formation of rat spleen cells and T cells, isolated by an Ig-anti-Ig column, was examined. It has been demonstrated that 90% of ARFC are B cells and 10% are T cells. The allorosette phenomenon can therefore be considered an immune reaction concerning predominantly the B-cell system. Allorosetteforming T cells are discussed to be SD-antigen-recognizing cytotoxic T cells.

Key words

Rosette formation B-lymphocytes T-lymphocytes Rats Transplantation (allogenic) 

Abbreviations used

ARFC

allorosette-forming cells

B

bursa equivalent-derived

BSA

bovine serum albumin

ICA

immuno-cyto-adherence

Ig

immunoglobulin

LD

lymphocyte defined

MEM

minimal essential medium

PBS

phosphate-buffered saline

SD

serologically defined

T

thymus-derived

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bach FH, Bach ML, Sondel PM (1976) Differential function of major histocompatibility complex antigens in T-lymphocyte activation. Nature 259:273–281PubMedGoogle Scholar
  2. 2.
    Bach JF, Dardenne M (1969) Signification du phénomène de rosette chez la souris non immunisée. CR Acad Sci (Paris) 269:751–754Google Scholar
  3. 3.
    Bach JF, Dardenne M (1972) Antigen recognition by T-lymphocytes. I. Thymus and marrow dependence of spontaneous rosette-forming cells in the mouse. Cell Immunol 3:1–10PubMedGoogle Scholar
  4. 4.
    Balch CM, Feldman JD (1974) Thymus-dependent (T-) lymphocytes in rat. J Immunol 112:79–86PubMedGoogle Scholar
  5. 5.
    Binz H, Lindenmann J, Wigzell H (1974) Cell-bound receptors for alloantigens on normal lymphocytes. I. Characterization of receptor-carrying cells by the use of antibodies to alloantibodies. J Exp Med 139:877–887PubMedGoogle Scholar
  6. 6.
    Biozzi G, Stiffel C, Mouton D, Liacopoulos-Briot M, Decreusefond C, Bouthillier Y (1966) Etude du phénomène de l'immuno-cyto-adherence au cours de l'immunisation. Ann Inst Pasteur [Suppl] 110:7–32Google Scholar
  7. 7.
    Brondz BD, Kotomina IF, Jeliseyeva LS, Egorova SG, Snegirova AE (1973) Relationship between killer and rosette-forming cells reactive to H-2 antigens. Scand J Immunol 2:463–477PubMedGoogle Scholar
  8. 8.
    Dostal G, Mohr R, Kinsky R, Hermann G (1972) Quantitative Bestimmung der Suppression antikörperbildender Milzzellen durch passives Enhancement nach Nierentransplantation bei Ratten. Langenbecks Arch Chir 332:115–117Google Scholar
  9. 9.
    Elliott BE, Haskill JS (1973) Characterization of thymus-derived and bone marrow-derived rosette-forming lymphocytes. Eur J Immunol 3:68–74PubMedGoogle Scholar
  10. 10.
    Elliott BE, Haskill JS, Axelrad MA (1973) Thymus-derived rosettes are not “helper” cells. J Exp Med 138:1133–1143PubMedGoogle Scholar
  11. 11.
    Elliott BE, Haskill JS (1975) Rosette-forming ability of thymus-derived lymphocytes in humoral and cell-mediated immunity. II. Helper cell activity. J Exp Med 141:600–607PubMedGoogle Scholar
  12. 12.
    Elliott BE, Haskill JS, Axelrad MA (1975) Rosette-forming ability of thymus-derived lymphocytes in cell-mediated immunity. I. Delayed hypersensitivity and in vitro cytotoxicity. J Exp Med 141:584–599PubMedGoogle Scholar
  13. 13.
    Elliott BE, Nagy Z, Nabholz M, Pernis B (1977) Antigen recognition by T cells activated in the mixed lymphocyte reaction: specific binding of allogeneic cell material after removal of surface-bound antigen by trypsin. Eur J Immunol 7:287–291PubMedGoogle Scholar
  14. 14.
    Fenner M, Frischknecht H, Binz H, Lindenmann J, Wigzell H (1979) Alloantigens derived from stimulator cells and bound onto MLC-activated rat T-lymphoblasts. Scand J Immunol 9:553–562PubMedGoogle Scholar
  15. 15.
    Grabar P, Williams CA, Jr (1955) Méthode immunoéléctrophorétique d'analyse de mélanges de substances antigéniques. Biochim Biophys Acta 17:67–74PubMedGoogle Scholar
  16. 16.
    Haskill JS (1972) Discussion paper: Studies on the differentiation within the immune system. Ann NY Acad Sci 207:57–72Google Scholar
  17. 17.
    Haskill JS, Elliott BE, Kerbel R, Axelrad MA, Eidinger D (1972) Classification of thymusderived and marrow-derived lymphocytes by demonstration of their antigen-binding characteristics. J Exp Med 135:1410–1415PubMedGoogle Scholar
  18. 18.
    Hermann G, Dostal G, Sesterhenn K (1970) Quantitative Bestimmung der individuellen zellularen Immunantwort bei Nieren-transplantierten Ratten. Naturwissenschaften 57:90–91Google Scholar
  19. 19.
    Hermann G, Sesterhenn K, Dostal G (1970) La réaction des cellules spleniques après transplantation chez le rat. Ann Inst Pasteur 119:135–136Google Scholar
  20. 20.
    Hermann G, Sesterhenn K, Dostal G, Mohr R (1971) Die Immunocytoadhärenz im allogenen System: Ein experimenteller Ansatz zur immunologischen Diagnose der Abstoßungskrise. Langenbecks Arch Chir 329:1035Google Scholar
  21. 21.
    Hermann G, Kinsky R, Dostal G, Vales AC (1972) Transplantation rénale chez le rat. Prolongation de la greffe et diminution de la réponse immunitaire cellulaire par facilitation passive. Ann Inst Pasteur 123:122Google Scholar
  22. 22.
    Hermann G, Parameritis J (1973) Bestimmung der Immunantwort im allogenen System mit Hilfe der Immunozytoadhärenz. MMW 115:1985–1987Google Scholar
  23. 23.
    Kontiainen S, Andersson LC (1975) Antigen-binding T cells as helper cells by immune rosette formation. J Exp Med 142:1035–1039PubMedGoogle Scholar
  24. 24.
    Krammer PH (1978) Alloantigen receptors on activated T cells in mice. I. Binding of alloantigens and anti-idiotypic antibodies to the same receptors. J Exp Med 147:25–38PubMedGoogle Scholar
  25. 25.
    Lengerová A, Viklický V, Rejzková J (1971) Specificity of the reaction between alloclusterforming cells and target erythrocytes. Folia Biol (Praha) 17:405–409Google Scholar
  26. 26.
    Lengerová A, Viklický V, Astorquiza MI (1972) Analysis of the specificity of alloclusterforming lymphocytes in allograftet mice. Folia Biol (Praha) 18:402–407Google Scholar
  27. 27.
    Micklem HS, Asfi C, Staines NA, Anderson N (1970) Quantitative study of cells reacting to skin allografts. Nature 227:947–949PubMedGoogle Scholar
  28. 28.
    Nagy Z, Elliott BE, Nabholz M, Krammer PH, Pernis B (1976) Specific binding of alloantigens to T cells activated in the mixed lymphocyte raction. J Exp Med 143:648–659PubMedGoogle Scholar
  29. 29.
    Nagy Z, Elliott BE, Nabholz M (1976) Specific binding of K- and I-region products of the H-2 complex to activated thymus-derived (T) cells belonging to different Ly subclasses. J Exp Med 144:1545–1553PubMedGoogle Scholar
  30. 30.
    Nairn RC (1976) Fluorescent protein tracing, 4th edn. Churchill Livingstone, Edinburgh London New YorkGoogle Scholar
  31. 31.
    Nota NR, Liacopoulos-Briot M, Stiffel C, Biozzi G (1964) L'immuno-cyto-adhérence: une méthode simple et quantitative pour l'étude in vitro des cellules productrices d'anticorps. CR Acad Sci Paris 259:1277–1280Google Scholar
  32. 32.
    Ouchterlony Ö (1958) Diffusion-in-gel methods for immunological analysis. Prog Allergy 5:1–78PubMedGoogle Scholar
  33. 33.
    Ouchterlony Ö (1962) Diffusion-in-gel methods for immunological analysis II. Prog Allergy 6:30–154PubMedGoogle Scholar
  34. 34.
    Parameritis J (1974) Untersuchungen über die Immunantwort und ihre passive biologische Behinderung bei Inkompatibilität im H1-System der Ratte. Inaug.-Diss. Univ. KölnGoogle Scholar
  35. 35.
    Swanborg RH, Hellström U, Perlmann H, Hammarström S, Perlmann P (1977) A new rat lymphocyte surface marker: Characterization and separation of cells with receptors for helix pomatia hemagglutinin. Scand J Immunol 6:235–239PubMedGoogle Scholar
  36. 36.
    Wigzell H (1976) Specific affinity fractionation of lymphocytes using glass or plastic bead columns. In: Natvig JB, Perlmann P, Wigzell H (eds) Lymphocytes: Isolation, fractionation and characterization. Scand J Immunol [Suppl] 5:23–30Google Scholar
  37. 37.
    Wilson JD (1973) The functions of immune T and B rosette-forming cells. Immunology 25:185–196Google Scholar
  38. 38.
    Zaalberg OB (1964) A simple method for detecting single antibody-forming cells. Nature 202:1231Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • K. W. Lempfrid
    • 1
  • G. Hermann
    • 1
  1. 1.Immunology Branch Dept. of SurgeryUniversity of CologneKölnFederal Republic of Germany

Personalised recommendations