Research in Experimental Medicine

, Volume 186, Issue 5, pp 343–352 | Cite as

The effect of fat-rich, protein-rich, and carbohydrate-rich diets combined with long-term ethanol ingestion on the pancreatic proteolytic proenzymes in rats

  • O. J. Rämö
  • P. Jalovaara
  • L. Kalevi Korhonen
Original Papers

Summary

The effect of chronic alcohol ingestion on pancreatic adaptation to protein-, fat-, carbohydrate-rich and standard diets in rats was studied. Alcoholic animals received 15% (v/v) ethanol in their drinking water and controls tap water for 12 weeks. No mortality or dehydration was observed. After this diet period pancreases were removed, and specific and total activities of trypsinogen and chymotrypsinogen activities of the homogenates were determined. Ethanol itself did not have any significant effect on chymotrypsinogen or trypsinogen activities, but when combined with special diets significant increases were observed. The activity of chymotrypsinogen increased most in the alcohol and fat-rich diet group, that of trypsinogen in the alcohol and carbohydrate-rich diet group.

Key words

Chymotrypsinogen Trypsinogen Pancreatic Proenzymes Diets Ethanol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ben Abdeljlil A, Visani AM, Desnuelle P (1963) Adaptation of the exocrine rat pancreas to the composition of the diet. Biochem Biophys Res Commun 10:112–116Google Scholar
  2. 2.
    Bordalo O, Noronha M, Dreiling DA (1977) Functional and morphologic studies of the effect of alcohol on the pancreatic parenchyma. Mt Sinai J Med 44:481–484PubMedGoogle Scholar
  3. 3.
    Bourdel G (1983) Effect of separate feeding of proteins and lipids on pancreatic adaptation in the rat. Am J Physiol 244:125–130Google Scholar
  4. 4.
    Braganza JM (1983) Pancreatic disease: a casualty of hepatic “detoxification”? Lancet 2:1000–1003PubMedGoogle Scholar
  5. 5.
    Desnuelle P, Reboud JP, Ben Abdeljlil A (1962) Influence of the composition of the diet on the enzyme content of rat pancreas. The exocrine pancreas. Normal and abnormal functions. Ciba Foundation Symposium, London, 90–114Google Scholar
  6. 6.
    Durbec JP, Sarles H (1978) Multicenter survey of the etiology of pancreatic diseases. Digestion 18:337–350PubMedGoogle Scholar
  7. 7.
    Emde C, Liehr R-M, Gregor M, Pleul O, Riecken EO, Menge H (1985) Lack of adaptive changes in human pancreatic amylase and lipase secretion in response to high-carbohydrate, low-fat diet applied by a 10-day continuous intraduodenal infusion. Dig Dis Sci 30:204–210PubMedGoogle Scholar
  8. 8.
    Glazer G, Steer ML (1977) Requirements for activation of trypsinogen and chymotrypsinogen in rabbit pancreatic juice. Anal Biochem 77:130–140PubMedGoogle Scholar
  9. 9.
    Goslin J, Hong SS, Magee DF, White TT (1965) Relationship between diet, ethyl alcohol consumption, and some activities of the exocrine pancreas in rats. Arch Int Pharmacodyn 157:462–469PubMedGoogle Scholar
  10. 10.
    Huttunen R, Korhonen LK (1973) New trypsin- and chymotrypsin-binding factor in rat plasma. Ann Med Exp Fenn 51:145–150PubMedGoogle Scholar
  11. 11.
    Isaksson G, Lilja P, Lundquist I, Ihse I (1983) Influence of dietary fiber on exocrine pancreatic function in the rat. Digestion 27:57–62PubMedGoogle Scholar
  12. 12.
    Jalovaara P, Huttunen R (1977) The effect of chronic ethanol ingestion on the pancreatic proteolytic enzymes and their inhibitors in the rat. Scand J Gastroenterol 12:785–792PubMedGoogle Scholar
  13. 13.
    Lahaie RG, Dagorn JC (1981) Dietary regulation of pancreatic protein synthesis. II. Kinetics of adaptation of protein synthesis and its effect on enzyme content. Biochim Biophys Acta 654:119–123PubMedGoogle Scholar
  14. 14.
    Lowry OH, Rosebrough NJ, Farr AL, Randall JR (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  15. 15.
    Marquis G, Marchetti J, Burlet C, Boulange M (1975) Secretion urinaire et hormone antidiuretique chez des rats soumis a une administration repetee d'ethanol. C R Soc Biol (Paris) 169:154–161Google Scholar
  16. 16.
    Marshall JB, Tavill AS, Louis LN, Markakis D (1982) The effect of ethanol on pancreatic protein synthesis and secretion. Gastroenterology [Abstr] 3:1125Google Scholar
  17. 17.
    Poort SR, Poort C (1981) Effect of feeding diets different composition on the protein synthetic pattern of the rat pancreas. J Nutr 111:1475–1479PubMedGoogle Scholar
  18. 18.
    Salman AJ, Dal Borgo G, Publos MH, McGinnis J (1967) Changes in pancreatic enzymes as a function of diet in the chick. Proc Soc Exp Biol Med 126:694–698Google Scholar
  19. 19.
    Sardesai VM, Orten JM (1968) Effect of prolonged alcohol consumption in rats on pancreatic protein synthesis. J Nutr 96:241–246PubMedGoogle Scholar
  20. 20.
    Sarles H, Lebreuil G, Tasso F, Figarella C, Clemente F, Devaux MA, Fagonde B, Payan H (1971) A comparison of alcoholic pancreatitis in rat and man. Gut 12:377–388PubMedGoogle Scholar
  21. 21.
    Snook JT (1965) Dietary regulation of pancreatic enzyme synthesis, secretion, and inactivation in the rat. J Nutr 87:297–305PubMedGoogle Scholar
  22. 22.
    Snook JT (1968) Pancreatic adaptation to change in dietary protein source in rats fed at different frequencies. J Nutr 94:351–360PubMedGoogle Scholar
  23. 23.
    Stifel FB, Rosensweig NS, Zakim D, Herman RH (1968) Dietary regulation of glycolytic enzymes. I. Adaptative changes in rat jejunum. Biochim Biophys Acta 170:221–227PubMedGoogle Scholar
  24. 24.
    Stock-Damge C, Raul F, Haegel P, Aprahamian M, Noriega R, Grenier JF (1983) Effect of chronic alcohol administration on the exocrine pancreas after jejunoileal bypass in the rat. Int J Obesity 7:459–466Google Scholar
  25. 25.
    Stöckmann F, Söling HD (1981) Regulation of biosynthesis of trypsinogen and chymotrypsinogen by nutritional and hormonal factors in the rat. Eur J Clin Invest 11:121–132PubMedGoogle Scholar
  26. 26.
    Temler RS, Dormond CA, Simon E, Morel B, Mettraux C (1984) Response of rat pancreatic proteases to dietary proteins, their hydrolysates and soybean trypsin inhibitor. J Nutr 114:270–278PubMedGoogle Scholar
  27. 27.
    Weesner RE, Ruffolo JJ, Murphy RF, Dincsoy HP, Mendenhall CL (1985) Effect of chronic ethanol consumption on the pancreas of the hamster. Dig Dis Sci 30:168–177PubMedGoogle Scholar
  28. 28.
    Wilson JS, Pirola RC (1983) Pathogenesis of alcoholic pancreatitis. Aust NZ J Med 13:307–312Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • O. J. Rämö
    • 1
  • P. Jalovaara
    • 2
  • L. Kalevi Korhonen
    • 1
  1. 1.Dept. of AnatomyUniversity of OuluOulu 22Finland
  2. 2.Dept. of SurgeryUniversity of OuluOuluFinland

Personalised recommendations