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Calcitonin and granulation tissue formation in the rat

An Experimental Study

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Research in Experimental Medicine

Summary

The effects of systemically administered calcitonin (CT) on granulation tissue development were compared using viscose cellulose sponges as wound model in the rat. The ingrowth of new granulation tissue was analyzed for the contents of various connective tissue components (total nitrogen, hydroxyproline, DNA, and RNA) and for the differential counts of the cells infiltrating the sponges.

The results indicate that up to 4 weeks postoperatively (p.o.) CT did not affect the net amount of collagen, measured as hydroxyproline, total nitrogen, aminosugars nor the dynamics of differentation and viability of the invading cells.

The present observation that CT has negligible effects, if any, on the early stages of connective tissue formation and on the cell population indicates that surgical procedures could be carried out safely during systemic CT therapy without an increased risk of wound complications.

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References

  1. Austin LA, Heath III H (1981) Calcitonin. Physiology and pathology. N Engl J Med 304:269–278

    PubMed  Google Scholar 

  2. Bethge JFJ, Babayan R, Borm HP, v. Fehrenteil R, tenHoff H, Hose H, Mangels P, Piening H, Reimers C, Wider U (1979) Versuch der biochemischen Beeinflussung der Frakturheilung im Tierexperiment. Res Exp Med (Berl) 175:197–222

    Google Scholar 

  3. Burton K (1956) A study of the conditions and mechanism of the diphenylamine reaction for the calorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–323

    PubMed  Google Scholar 

  4. Ceriotti G (1955) Determination of nucleic acids in animal tissues. J Biol Chem 214:59–67

    PubMed  Google Scholar 

  5. Doepfner WEH (1983) Pharmacological effects of calcitonin. Triangle 22:57–67

    Google Scholar 

  6. Duriez J (1979) Calcitonine et fracture. Ann Chir 33:3–7

    PubMed  Google Scholar 

  7. Ekeland A, Undertal T (1983) Effects of salmon calcitonin on synthesis and mineralization of collagen in rats. Acta Orthop Scand 54:470–478

    PubMed  Google Scholar 

  8. Ekeland A, Gautvik KM, Undertal T (1983a) Calcitonin producing tumour. Effects on fracture repair and normal bone in rats. Acta Orthop Scand 54:760–767

    PubMed  Google Scholar 

  9. Ekeland A, Myhre L, Undertal T (1983b) Effects of salmon calcitonin on mechanical properties of healing and intact bone and skin in rats. Acta Orthop Scand 54:462–469

    PubMed  Google Scholar 

  10. Lindgren JU, Narechania RG, McBeath AA, Lange TA, DeLuca HP (1981) Effects of 1,25 dihydroxyvitamin D3 and calcitonin on fracture healing in adult rats. Clin Orthop 160:304–308

    PubMed  Google Scholar 

  11. Lupulescu A, Habowsky J (1978a) Effects of calcitonin on wound healing: A morphological study in rabbits. J Surg Res 25:260–268

    PubMed  Google Scholar 

  12. Lubulescu A, Habowsky J (1978b) Effects of calcitonin on epidermal regeneration and collagen synthesis in rabbits with experimental wounds. Exp Pathol 16:291–302

    Google Scholar 

  13. MacIntyre I (1983) The physiological actions of calcitonin. Triangle 22:69–74

    Google Scholar 

  14. Mørk Hansen T, Lorenzen I (1975) Collagen profile in rat granulation tissue after cyclophosphamide treatment. Acta Pharmacol Toxicol 37:402–414

    Google Scholar 

  15. Pallin B, Ahonen J, Rank F, Zederfelt B (1975) Granulation tissue formation in oophorectomized rats treated with female sex hormones. Acta Chir Scand 141:702–709

    PubMed  Google Scholar 

  16. Pallin B, Ahonen J, Rank F, Zederfelt B (1975) Granulation tissue formation in viscose cellulose sponges of different design. Acta Chir Scand 141:697–701

    PubMed  Google Scholar 

  17. Pikkarainen J (1968) The molecular structure of vertebrate skin collagens. A comparative study. Acta Physiol Scand [Suppl] 309:1–72

    Google Scholar 

  18. Robert L, Brechemier D, Godeau G, Labat LM, Milhaud G (1977) Prevention of experimental immunoarteriosclerosis by calcitonin. J Pharmacol 26:2129–2135

    Google Scholar 

  19. Roberts PJ, Häyry P (1976) Sponge matrix allografts. A model for analysis of killer cells infiltrating mouse allografts. Transplatation 21:437–445

    Google Scholar 

  20. Salmela K, Roberts PJ, Leutenschlaeger I, Ahonen J (1980) The effect of local methylprednisolone on granulation tissue formation. Acta Chir Scand 146:451–544

    Google Scholar 

  21. Schmidt G, Thannhauser SJ (1945) A method for the determination of deoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissue. J Biol Chem 161:83–89

    Google Scholar 

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Authors and Affiliations

  1. Surgical Hospital, Division of Orthopedic Surgery and Traumatology, University Central Hospital, Kasarminkatu 11-13, SF-00130, Helsinki, Finland

    P. Paavolainen, T. Taivainen & T. Holmström

  2. Fourth Dept. of Surgery, University of Helsinki, Finland

    P. J. Roberts

  3. University of Turku, Finland

    R. Penttinen

Authors
  1. P. Paavolainen
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  2. T. Taivainen
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  3. T. Holmström
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  4. P. J. Roberts
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  5. R. Penttinen
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Paavolainen, P., Taivainen, T., Holmström, T. et al. Calcitonin and granulation tissue formation in the rat. Res. Exp. Med. 188, 41–48 (1988). https://doi.org/10.1007/BF01852093

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  • DOI: https://doi.org/10.1007/BF01852093

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