Summary
A technique employing silica particles was used to remove cells producing endogenous colony-stimulating factor (CSF) to allow measurement of the level of colony-forming units in culture (CFU-C) in human bone marrow cells. In comparison with the glass-adherence technique, this new approach resulted in a more complete degree of removal of CSF-producing cells and formation of more colonies. This method seems to be useful for performing an accurate assay of exogenous CSF.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Golde DW, Cline MJ (1974) Identification of the colony-stimulating cells in human peripheral blood. J Clin Invest 5:2981–2983
Niho Y, Till JE, McCulloch EA (1975) Granulopoietic progenitors in suspension culture: A comparison of stimulatory cells and conditioned media. Blood 45:811–821
Messner HA, Till JE, McCulloch EA (1973) Interacting cell populations affecting granulopoietic colony formation by normal and leukemic marrow. Blood 42:701–708
Ohhara N, Okamura S, Niho Y, Otsuka T (1985) Stimulatory effect of an aminopeptidase inhibitor on human bone marrow CFU-C mediated by the interaction of T-lymphocytes. Acta Haematol Jpn 48:134–138
Kumagai K (1981) Isolation of blood lymphocytes. Clin Immunol [Suppl 3] 13:328–332 (in Japanese)
Furusawa S, Komatsu H, Saito K, Enokihara H, Hirose K, Shishido H (1978) Effect of normal human serum on granulocyte colony formation by human bone marrow cells. J Lab Clin 91:377–386
Abo T (1983) Methods of identification, separation, and depletion of monocytes. In: Yata J, Fujiwara M (eds) The assay methods of lymphocyte function (in Japanese). Chugai Igakusha, pp 197–198
Kumagai K, Itoh K, Hinuma S (1979) Pretreatment of plastic petri dishes with fetal calf serum. A simple method for macrophage isolation. J Immunol Methods 29:17–20
Okamura S, Crane F, Jamel N, Messner HA, Mak TW (1980) Single-cell immunofluorescence assay for terminal transferase. Br J Cancer 41:159–167
Burgess AW, Wilson EAM, Metcalf D (1977) Stimulation by human placental conditioned medium of human hemopoietic colony formation by human marrow cells. Blood 49:573–583
Iscove NN, Senn JS, Till JE, McCulloch EA (1971) Colony formation by normal and leukemic human marrow cells in culture: Effect of conditioned medium from leukocytes. Blood 37:1–5
Kurland JI, Broxmeyer HE, Pelus LM, Bockman RS, Moore MAS (1978) Role for monocyte-macrophage-derived colony-stimulating factor and prostaglandin E in the positive and negative feedback control of myeloid stem cell proliferation. Blood 52:388–407
Suda T, Mizoguchi H, Miura Y (1981) Suppression of in vitro granulocyte-macrophage colony formation by the peripheral mononuclear phagocytic cells of patients with idiopathic aplastic anemia. Br J Haematol 47:433–442
Author information
Authors and Affiliations
Additional information
Partly supported by grants from the Ministry of Education, Science, and Culture of Japan (Nos. 60770949, 61015074, 61440054, 63015063) and from the Fukuoka Anti-Cancer Society
Rights and permissions
About this article
Cite this article
Ohhara, N., Okamura, S., Hayashi, S. et al. Granulocyte-macrophage colony formation in vitro using human non-phagocytic bone marrow cells. Res. Exp. Med. 188, 405–409 (1988). https://doi.org/10.1007/BF01851998
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01851998