Age-dependent increase in arterial smooth muscle calcium in spontaneously hypertensive rats
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Alterations in cellular calcium metabolism in essential hypertensive and in the SHR have been described. In the present study, particle-induced X-ray emission (PIXE) was used to get some information on the spatial distribution of Ca2+ in aortas of spontaneously hypertensive rats (SHR) and normotensive controls aged 1 week, 4 weeks, and 12 weeks.
It was found that the Ca2+ content was not elevated in the aortic smooth muscle of SHR aged 1 week (n = 9) as compared to normotensive controls (n = 8) (186.8 ± 89.9 µg Ca2+/g tissue vs 254.0 ± 73.7 µg Ca2+/g tissue. The Ca2+ content was significantly raised in the aortic smooth muscle of SHR aged 4 weeks (n = 9) as compared to 4-weeks-old WKY rats (n = 12) (726.0 ± 130.4 Ca2+/g tissue vs 440.3 ± 214.4 Ca2+ µg/g tissue and in SHR aged 3 months (n = 15) as compared to WKY rats (n = 12), respectively (3317.0 ± 734.0 µg Ca2+/g tissue vs. 1632.0 ± 569.6 µg Ca2+/g tissue).
The results confirm the age-related increase in the arterial Ca2+ content in normotensive rats and demonstrate additionally that this age-related rise in arterial Ca2+ content is accelerated in SHR.
Key wordsCa2+ Arterial smooth muscle Spontaneously hypertensive rats
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