Research in Experimental Medicine

, Volume 188, Issue 6, pp 397–403 | Cite as

Age-dependent increase in arterial smooth muscle calcium in spontaneously hypertensive rats

  • C. Spieker
  • W. Zidek
  • D. B. von Bassewitz
  • D. Heck
Original Papers


Alterations in cellular calcium metabolism in essential hypertensive and in the SHR have been described. In the present study, particle-induced X-ray emission (PIXE) was used to get some information on the spatial distribution of Ca2+ in aortas of spontaneously hypertensive rats (SHR) and normotensive controls aged 1 week, 4 weeks, and 12 weeks.

It was found that the Ca2+ content was not elevated in the aortic smooth muscle of SHR aged 1 week (n = 9) as compared to normotensive controls (n = 8) (186.8 ± 89.9 µg Ca2+/g tissue vs 254.0 ± 73.7 µg Ca2+/g tissue. The Ca2+ content was significantly raised in the aortic smooth muscle of SHR aged 4 weeks (n = 9) as compared to 4-weeks-old WKY rats (n = 12) (726.0 ± 130.4 Ca2+/g tissue vs 440.3 ± 214.4 Ca2+ µg/g tissue and in SHR aged 3 months (n = 15) as compared to WKY rats (n = 12), respectively (3317.0 ± 734.0 µg Ca2+/g tissue vs. 1632.0 ± 569.6 µg Ca2+/g tissue).

The results confirm the age-related increase in the arterial Ca2+ content in normotensive rats and demonstrate additionally that this age-related rise in arterial Ca2+ content is accelerated in SHR.

Key words

Ca2+ Arterial smooth muscle Spontaneously hypertensive rats 


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  1. 1.
    Zidek W, Losse H, Dorst KG, Zumkley H, Vetter H (1982) Intracellular sodium and calcium in essential hypertension. Klin Wochenschr 60:859–862PubMedGoogle Scholar
  2. 2.
    Erne P, Bolli P, Bürgisser E, Bühler FR (1984) Correlation of platelet calcium with blood pressure. N Engl J Med 310:1084–1088PubMedGoogle Scholar
  3. 3.
    Furspan PB, Bohr DF (1986) Calcium-related abnormalities in lymphocytes from genetically hypertensive rats. Hypertension [Suppl 2]:8 I-123–II-126Google Scholar
  4. 4.
    Bruschi G, Bruschi ME, Caroppo M, Orlandini G, Spaggiori M, Covatora A (1985) Cytoplasmatic free (Ca2+) is increased in platelets of spontaneously hypertensive rats and essential hypertensive patients. Clin Sci 68:179–184PubMedGoogle Scholar
  5. 5.
    Zidek W, Kerenyi T, Losse H, Vetter H (1983) Intracellular Na+ and Ca2+ in aortic smooth muscle cells after enzymatic isolation in spontaneously hypertensive rats. Res Exp Med (Berl) 183:129–132Google Scholar
  6. 6.
    Heck D (1979) The Karlsruhe proton microbeam system. Beitr Elektroenmikrosk. 12:259–262Google Scholar
  7. 7.
    Heck D, Rokita E (1984) Local matrix mass thickness determination in scanned micro PIXE by proton backscattering. Nucl Instrum Methods 3:259–262Google Scholar
  8. 8.
    Spieker C, Heck D, Zidek W, Stratmann T, v Bassewitz DB, Losse H, Vetter H, Zumkley H (1986) Elemental and trace elemental distrubution in medical samples: Analysis by proton-induced X-ray emission. Methods Find Exp Clin Pharmacol 8:363–366PubMedGoogle Scholar
  9. 9.
    Zidek W, Vetter H, Dorst KG, Zumkley H, Losse H (1982) Intracellular Na+ and Ca2+ activities in essential hypertension. Clin Sci 63:41s-43sGoogle Scholar
  10. 10.
    Postnov YV, Orlov SN (1985) Physiol Rev 65:904–944PubMedGoogle Scholar
  11. 11.
    Massingham R, Shevde S (1973) The ionic composition of aortic smooth muscle from A.S. hypertensive rats. Br J Pharmacol 48:422–424Google Scholar
  12. 12.
    Tobian L, Chesley G (1966) Calcium content of arteriolar walls in normotensive and hypertensive rats. Proc Soc Exp Biol Med 121:340–344PubMedGoogle Scholar
  13. 13.
    Jones AW (1974) Altered ion transport in large and small arteries from SHR and the influence of Ca. Circ Res [Suppl 1] 34/35:117–122Google Scholar
  14. 14.
    Bhalla RC, Webb C, Singh D, Ashley T, Brock T (1978) Calcium fluxes, calcium binding and adenosine cyclic 3′5′-monophosphate-dependent protein kinase activity in the aorta of spontaneously hyperternsive and Kyoto-Wistar-normotensive rats. Mol Pharmacol 14:468–477PubMedGoogle Scholar
  15. 15.
    Fleckenstein A, Frey M, Fleckenstein-Grün G (1986) Antihypertensive and arterial anticalcinosis effects of calcium antagonists. Am J Cardiol 57:1D-10DGoogle Scholar
  16. 16.
    McCarron J, Yung NN, Ugoretz BA (1981) Disturbances of calcium metabolism in the spontaneously hypertensive rat. Hypertension 3:1162–1167Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • C. Spieker
    • 1
  • W. Zidek
    • 1
  • D. B. von Bassewitz
    • 2
  • D. Heck
    • 3
  1. 1.Medizinische Universitäts-PoliklinikWestfälischen Wilhelms-Universität MünsterMünsterFederal Republic of Germany
  2. 2.Gerhard Domagk Institut für PathologieWestfälischen Wilhelms-Universität MünsterMünsterFederal Republic of Germany
  3. 3.Kernforschungszentrum KarlsruheKarlsruheFederal Republic of Germany

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