Research in Experimental Medicine

, Volume 185, Issue 4, pp 269–276 | Cite as

Reaction of human leukocytes after in vitro contact with a glucocorticoid

  • H. Schmidt
  • U. Wagner
Original Papers

Summary

The used hormone prednisolone bisuccinate causes in the concentration range of 2–1,000 µg/ml visible changes of the cell morphology and enzymatic activity in all leukocytes. These alterations appear immediately after the application of the glucocorticoid and remain nearly constant during the time of investigation (1 h). The changes in the cell morphology and the loss of enzymatic activity increase with enhanced hormone concentration. The following reproducible morphological changes caused by the hormone comprise swelling, vacuolization and deformation of the nuclei with concomitant release of nuclei material. In addition, cloverleaf and fircone-like structures originate in connection with the formation of small bubbles.

Key words

Leukocytes Glucocorticoids Defence reactions 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baume LJ, Fiore-Donno G (1970) The effects of corticosteroids on the dental pulp. In: Staple PH (ed) Advances in oral biology, vol 4. Academic Press, New York London, pp 111–129Google Scholar
  2. Baxter JD, Rousseau GG (1979) Glucocorticoid hormone action: An overview. In: Baxter JD, Rousseau GG (eds) Glucocorticoid action. Springer, Berlin Heidelberg New York, pp 1–24Google Scholar
  3. Fauci AS (1979) Immunosuppressive and anti-inflammatory effects of glucocorticoids. In: Baxter JD, Rousseau GG (eds) Glucocorticoid action. Springer, Berlin Heidelberg New York, pp 449–465Google Scholar
  4. Harris AW, Baxter JD (1979) Variations in cellular sensitivity to glucocorticoids: observations and mechanism. In: Baxter JD, Rousseau GG (eds) Glucocorticoid action. Springer, Berlin Heidelberg New York, pp 423–448Google Scholar
  5. Jesserer H (1973) Cortisonschäden und Cortisonismus. Rheuma Forum 1. Braun, KarlsruheGoogle Scholar
  6. Labhart A (1978) Die Nebennierenrinde. In: Labhart A (Hrsg) Klinik der inneren Sekretion, 3. Aufl. Springer, Berlin Heidelberg New York, S 286–422Google Scholar
  7. Schmidt H (1981) Zum histochemischen Nachweis von Phenoloxidase in menschlichen Blutzellen. Folia Haematol (Leipz) 108:76–84Google Scholar
  8. Schmidt H (1983) Phenoloxidase—a specific enzyme of defence cells. Wiss Z Univ Halle 30:12–22Google Scholar
  9. Schmidt H, Módis L (1970) Das Verhalten verschiedener Dehydrogenasen und Oxydasen der Rattenhaut bei lokaler Ischämie Acta Histochem 37:80–104PubMedGoogle Scholar
  10. Schmidt H, Módis L (1974) Der Nachweis von Phenoloxidase enthaltenden Zellen (= POZ) in verschiedenen Geweben und Organen der Ratte. Acta Histochem 51:286–300Google Scholar
  11. Schmidt H, Kiss FA (1975) Ein Beitrag zur Methodik des Phenoloxidasenachweises. Acta Histochem 53:306–314PubMedGoogle Scholar
  12. Spector G (1981) Pharmacological properties of the glucocorticoids. Int Dent J 31:152–155PubMedGoogle Scholar
  13. Thompson EB (1979) Glucocorticoids and lysosomes. In: Baxter JD, Rousseau GG (eds) Glucocorticoid action. Springer, Berlin Heidelberg New York, pp 575–581Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • H. Schmidt
    • 1
  • U. Wagner
    • 2
  1. 1.Physiologisch-chemisches Institut der MLUHalle (Saale)German Democratic Republic
  2. 2.Abt. f. Chirurgische Stomatologie und Kiefer-GesichtschirurgieKlinik und Poliklinik für Stomatologie an der MLUHalle (Saale)German Democratic Republic

Personalised recommendations