Research in Experimental Medicine

, Volume 182, Issue 3, pp 203–213 | Cite as

The adaptive response of disaccharidase activities at different sites along the villus epithelium after proximal intestinal resection in the rat

A Microdensitometric Study of Enzyme Kinetics
  • S. Gutschmidt
  • W. Kaul
  • H. Menge
  • E. O. Riecken
Original Papers

Summary

The “in situ” kinetic constants (app.\(\bar K_m\) and\(\bar V_{max}\)) of brush border neutralα-glucosidase (EC 3.2.1.20) and lactase/β-glucosidase (EC 3.2.1.21) were determined 4, 6 (onlyα-glucosidase), and 12 days after 60% proximal intestinal resection in rat ileum at the villus base and the transition zone between middle and upper villus third by use of a quantitative biochemical analysis of enzymes in tissue sections (section biochemistry). Sham-operated rats served as controls, and the kinetic data (means per rat, time and villus position) were compared (n=4 animals in each experimental group) first by an overall factorial analysis of variance and thereafter in detail using nonparametric test procedures.

Both enzyme activities exhibited a differential response: No changes of lactase/β-glucosidase kinetics, but a significant decrease in both\(\bar V_{max}\)- and\(\bar K_m\)-values of neutralα-glucosidase, which was already fully expressed on day 4 after resection and confined to the apical villus region still implying a basoapical increase of\(\bar V_{max}\) and thus maintaining the normal activity gradient on a lower level.

In conclusion, a complex pattern of enzymatic adaptation to proximal intestinal resection ensues in the hyperplastic ileal mucosa which cannot be explained simply in terms of the hypothesis of cellular immaturity.

Key words

Disaccharidases Section biochemistry Mucosal hyperplasia Intestinal adaptation 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • S. Gutschmidt
    • 1
  • W. Kaul
    • 1
  • H. Menge
    • 1
  • E. O. Riecken
    • 1
  1. 1.Abteilung für Innere Medizin mit Schwerpunkt GastroenterologieKlinikum Steglitz der Freien Universität BerlinBerlin 45Germany

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