Summary
Two groups of dogs received daily intravenous doses of 20 mg/kg 131-I-labelled horse-anti-dog lymphcyte globulin or normal horse gammaglobulin respectively over a period of 11 days. Horse-anti-dog lymphocyte globulin showed a significantly higher eleimination rate than normal horse gammaglobulin. In contrary to the continuous increase in serum radioactivity during normal horse gammaglobulin treatment, there was a plateau after the 5th day in the horse-anti-dog lymphocyte globulin group. The xenogeneic protein concentration, measured with the single radial immunodiffusion technique, at the end of treatment was 165 ± 8 mg/1 in the horse-anti-dog lymphocyte globulin, compared to 498 ± 15 mg/1 in the normal horse gammaglobulin group. After treatment horse-anti-dog-lymphcyte globulin treated animals showed a significantly higher increase in active hemagglutination titer against horse erythrocytes with an average of 2−8.
Zusammenfassung
Zwei Gruppen von Hunden wurden elf Tage lang mit 20 mg/kg 131-I-markiertem Pferde-anti-human-Lymphozyten-Globulin bzw. Normal-Pferde-Gammaglobulin i.v. behandelt. Pferde-anti-human-Lymphozyten-Globulin wurde signifikant schneller eliminiert als Normal-Pferde-Gammaglobulin. Im Gegensatz zu dem kontinuierlichen Anstieg der Serum-Radioaktivität unter Normal-Pferde-Gammaglobulin-Behandlung war in der Pferde-anti-human-Lymphozyten-Globulin-Gruppe ab dem 5. Tag eine Plateaubildung zu beobachten. Zum Behandlungsende betrug die Serumkonzentration des xenogenen Proteins 165 ± 8 mg/1 in der Pferde-anti-human-Lymphozyten-Globulin- und 498 ± 15 mg/1 in der Normal-Pferde-Gammaglobulin-Gruppe. Die Pferde-anti-human-Lymphozyten-Globulin-behandelten Tiere zeigten einen signifikant höheren Anstieg agglutinierender Antikörper gegen Pferde-Erythrozyten als Normal-Pferde-Gammaglobulin-behandelte Tiere. Diese starke Immunogenizität von antilymphozytärem Pferde-Gammaglobulin muß bei der Applikation von Pferde-anti-human-Lymphozyten-Globulin berücksichtigt werden.
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Abbreviations
- ALG:
-
horse-anti-dog lymphocyte globulin
- NHG:
-
normal horse gammaglobulin
References
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Zink, R., Seifert, J., Ring, J. et al. Cumulation and elimination of horse-anti-dog lymphocyte and normal horse gammaglobulin in dogs. Res. Exp. Med. 167, 231–238 (1976). https://doi.org/10.1007/BF01851647
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DOI: https://doi.org/10.1007/BF01851647