Research in Experimental Medicine

, Volume 177, Issue 1, pp 71–78 | Cite as

Growth and treatment of B-16 melanoma in hyperglycemic mice

  • J. Pavelić
  • B. Benković
  • K. Pavelić
Original Works / Originalarbeiten


Melanoma B-16 grew slowly in mice with hyperglycemia induced by alloxan, glucagon, or glucose. The mechanism of retarded tumor growth is different and depends on the origin of hyperglycemia. The concentration of immunoreactive insulin in blood of mice with melanoma and in the tumor tissue is increased in nondiabetic as well as in diabetic mice. The chemotherapy of melanoma in diabetic mice is as effective as in nondiabetic mice whereas immunotherapy in diabetic mice is not effective. Combined chemoimmunotherapy of melanoma in diabetic mice is more effective than either therapy alone only when mice are given a daily dose of insulin.

Key words

Melanoma Hyperglycemia Diabetes mellitus Antitumor therapy 


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  1. 1.
    Botzenhardt U, Lemmel EM (1978) Kinetics of the reactive cell clones after immunosuppression and induction of tolerance: (1) inhibition of 19S and 7S plaque-forming cells in the primary and secondary immune response to sheep red blood cells by cyclophosphamide and 036.5122 (Asta) Agents Action 5:512–518Google Scholar
  2. 2.
    Goldman RD, Kaplam NO, Hall TC (1964) Lactic dehydrogenase in human neoplastic tissue. Cancer Res 24:389–399PubMedGoogle Scholar
  3. 3.
    Goranson ES, Willms M (1954) Inhibition of growth of transplanted hepatomas in alloxanised Wistar rats. Cancer Res 14:730–733PubMedGoogle Scholar
  4. 4.
    Goranson ES, Tilser GJ (1955) Studies of the relationship of alloxan-diabetes and tumor growth. Cancer Res 15:626–631PubMedGoogle Scholar
  5. 5.
    Geran RI, Greenberg NH, Macdonald MM, Schumacher AM, Abbot BJ (1972) Protocols for screening chemical agents and natural products against animal tumors and other biological systems. Cancer Chemother Rep 3:11–13Google Scholar
  6. 6.
    Hyvarinen A, Nikkila BA (1962) Specific determination of blood glucose with Ø-toluidine. Clin Chim Acta 7:140–143PubMedGoogle Scholar
  7. 7.
    Jehl JA, Mayer J, McKee RW (1955) Influence of the hereditary obese-hyperglycemic syndromes and of alloxan diabetes on the survival of mice with Ehrlich ascites carcinoma. Cancer Res 15:341–343PubMedGoogle Scholar
  8. 8.
    Morgan CR, Lazarow A (1963) Immunoassay of insulin: two antibody systems. Plasma insulin levels of normal, subdiabetic, and diabetic rats. Diabetes 12:115–126Google Scholar
  9. 9.
    Pavelić K, Slijepčević M (1978) Growth of a thymoma in diabetic mice treated with insulin. Eur J Cancer 14:675–679PubMedGoogle Scholar
  10. 10.
    Pavelić K, Slijepčević M, Pavelić J (1978) Recovery of immune system in diabetic mice after treatment with insulin. Horm Metab Res 10:381–386PubMedGoogle Scholar
  11. 11.
    Pavelić K, Hršak I (1978) Effect of immunosuppression on the growth of six murine tumors. Z Krebsforsch 92:147–156Google Scholar
  12. 12.
    Pavelić K (1979) Aplastic carcinoma in diabetic mice: hyperglycemia suppressed proliferation rate and insulin synthesis by tumor cells. J Natl Cancer Inst 62:139–141PubMedGoogle Scholar
  13. 13.
    Pavelić K, Pavelić J (1980) Glucagon suppressed proliferation rate of mammary aplastic carcinoma in mice. Horm Metab Res 12:243–246PubMedGoogle Scholar
  14. 14.
    Pavelić J, Pavelić K (1980) Insulin-stimulated phagocytic ability and humoral immunological response in mice. Horm Metab Res 12:42PubMedGoogle Scholar
  15. 15.
    Sablina II, Zilbere AM, Zidermane AA (1975) The effect of alloxan diabetes on the growth of transplantable tumors. Latv PSR Zinat Akad Vestis 1:53–58Google Scholar
  16. 16.
    Shapot VS (1972) Some biochemical aspects of the relationship between the tumor and the host. Adv Cancer Res 15:253–286PubMedGoogle Scholar
  17. 17.
    Turkington RW, Ward OT (1969) DNA polymerase and DNA synthesis in mammary carcinoma cells. Biochem Biophys Acta 174:282–290PubMedGoogle Scholar
  18. 18.
    Weber MJ (1973) Hexose transport in normal and in Rous sarcoma virus-transformed cells. J Biol Chem 24:2978–2983Google Scholar
  19. 19.
    Welsch CW, Delturri BC, Brennan MJ (1976) DNA synthesis of human, mouse and rat mammary carcinomas in vitro. Cancer 38:1272–1281PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • J. Pavelić
    • 1
  • B. Benković
    • 1
  • K. Pavelić
    • 1
  1. 1.Department of Experimental Biology and MedicineRudjer Bošković InstituteZagrebYugoslavia

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