Research in Experimental Medicine

, Volume 177, Issue 1, pp 33–41 | Cite as

Large-scale purification of IgM from human sera

Comparison of Three Optimized Procedures Utilizing Protein A Chromatography
  • Harald Mauch
  • Gunther Kümel
  • Hans-Joachim Hammer
Original Works / Originalarbeiten

Summary

For the preparation of gram amounts of IgM from human sera sedimention at 100,000g or treatment with ZnSO4 of the redissolved “euglobulin”-precipitate was compared to direct precipitation from the clarified serum by boric acid. Three alternative large scale purification procedures were developed, leading to an IgM-sample characterized as pure by various criteria. Inclusion of protein A chromatography proved to enhance the yield very considerably.

Key words

IgM Purification Methods 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Harald Mauch
    • 1
  • Gunther Kümel
    • 1
  • Hans-Joachim Hammer
    • 1
  1. 1.I. Med. Klinik u. PoliklinikUniversität des SaarlandesHomburgFederal Republic of Germany

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