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Research in Experimental Medicine

, Volume 171, Issue 3, pp 229–241 | Cite as

The early phase of insulin release in man — a new method for quantitative analysis

  • H. Dörfler
  • G. Wolfram
  • N. Zöllner
Article

Summary

A method is presented for the quantitative analysis of early insulin release in man. There were measured arterial insulin levels after glibornuride administered intravenously. The mathematical procedure has been modified: Modification I is based on the assumption that early insulin release represents a wave like insulin delivery, modification II is based on the assumption that this insulin bolus is immediately followed by a slower insulin release which must be distinguished from the second phase of insulin release. For the calculations there was used a “primary insulin space” derived from experiments with exogenous insulin. The results of calculations were varying up to 1.5 units of early insulin release in healthy volunteers receiving glibornuride with dosages varying up to 50 mg. The value of the presented method for examinations of insulin release for theoretical and clinical purposes is discussed.

Key words

Arterial insulin Dye dilution Insulin release in man Primary insulin space Quantitative analysis Sulfonylurea 

List of Abbreviations

S

Start of injection of Glibornuride.

D

Moment of insulin delivery into vena cava inferior.

tSD

(Table 3, Fig. 2, 3) The estimated time between sulfonylurea injection at startpoint S and insulin delivery into vena cava inferior at the moment D.

InsDsu

(Table 3, Fig. 2) The fictive insulin concentration at the moment D calculated by backward extrapolation from the decline of insulin levels after sulfonylurea.

InsDins

The fictive insulin concentration at the moment D using the mean ofk-values from decline of exogenous insulin for calculation.

ksu

(Table 3) Thek-value for the decline of insulin levels after sulfonylurea.

kIns

(Table 2) Thek-value for the decline of insulin levels after administration of exogenous insulin.

Area Ins

(Fig. 3) The area under the calculated decline of insulin levels after sulfonylurea using the averagek-value for the decline of insulin levels after exogenous insulin.

Area tot

(Fig. 3) The area under the curve describing the decline of insulin levels after sulfonylurea.

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • H. Dörfler
    • 1
  • G. Wolfram
    • 1
  • N. Zöllner
    • 1
  1. 1.Medizinische Poliklinik der UniversitätMünchen 2Federal Republic of Germany

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