Summary
Combined treatment with oral cholesterol (1 g/day) and i.v. Tween 80 (three times per week) in rabbits, induces a marked parenchymal thesaurismosis with hyperchylomicronemia. Plasma cholesterol and triglyceride levels do not differ from those of rabbits treated with cholesterol only. Rabbits with the combined treatment show no signs of atherosclerosis, but termination of Tween administration elicits a very severe atheromatosis.
Similarities of these experimental observations with the clinical findings of Fredrickson's type V disease, where hyperchylomicronemia is not accompanied by atherosclerosis, indicate that this is a useful experimental model for the rare clinical disease.
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Antonini, F. M., Weber, G., Zampi, G.: Biochemical and morphological studies on the hyperlipemia, xanthomatosis, and endogenous experimental atherogenesis in rabbits, following a combined treatment with Tween 80 and cholesterol. In: Reticulo-endothelial structure and function, J. H. Heller, ed., p. 431. New York: Ronald Press 1958
Block, W. D., Jarrett, J., Jr., Levine, J. B.: Use of a single color reagent to improve the automated determination of serum cholesterol. In: Technicon symposia on automation in analytical chemistry, L. T. Skeggs, ed., p. 345. New York: Mediad Inc. 1965
Fredrickson, D. S., Levy, R. I., Lees, R. S.: Fat transport in lipoproteins — An integrated approach to mechanisms and disorders. New Engl. J. Med.276, 32, 94, 148, 215, 273 (1967)
Fredrickson, D. S., Levy, R. I.: Familial hyperlipoproteinemia. In: The metabolic basis of inherited disease, J. B. Stanburyet al., eds., p. 600. New York: McGraw Hill 1972
Havel, R. J., Eder, H. A., Bragdon, J. H.: The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J. clin. Invest.34, 1345 (1955)
Hawk, P. B., Oser, B. L., Summerson, W. H.: Practical physiological chemistry, p. 555. London: Churchill 1954
Hyvarinen, A., Nikkila, E.: Specific determination of blood glucose with Otoluidine. Clin. chim. Acta7, 140 (1962)
McLellan, G. H.: Automated colorimetric method for estimating serum triglycerides. Clin. chim. Acta17, 535 (1971)
Noble, R. P.: Electrophoretic separation of plasma lipoproteins in agarose gel. J. Lipid Res.91, 693 (1968)
Payne, T. P. B., Duff, G. L.: Effect of tween 80 on serum lipids and the tissues of cholesterol-fed rabbits. Arch. Path.51, 358 (1951)
Rouser, G., Siakotos, A. N., Fleischer, R. S.: Quantitative analysis of phospholipids by thin layer chromatography and phosphorus analysis of spots. Lipids1, 85 (1966)
Scanu, A., Oriente, P., Szajewski, J., McCormack, J. L., Page, I. H.: Triton hyperlipemia in dogs. J. exp. Med.114, 279 (1961)
Schoenheimer, R.: Über die experimentelle Cholesterinkrankheit der Kaninchen. Virchows Arch. path. Anat.249, 1 (1924)
Selye, H.: Stress. Acta endocr. (Montreal) (1950)
Weber, G., Zampi, G.: La malattia ateromasica “endogena” del coniglio conseguente alla reversione della xantomatosi sperimentale splancno-tesaurotica non aterogena. Arch. “De Vecchi”24, 1063 (1956)
Weber, G., Zampi, G.: L'influsso del Tween 80 sulla reversione della xantomatosi etc. Arch. De Vecchi26, 563 (1957)
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Dr. A. Barbaro is acknowledged for his help.
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Weber, G., Resi, L., Agradi, E. et al. An experimental model for type V hyperlipoproteinemia: Combined tween and cholesterol treatment in rabbits. Res. Exp. Med. 161, 304–310 (1973). https://doi.org/10.1007/BF01851454
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DOI: https://doi.org/10.1007/BF01851454