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Research in Experimental Medicine

, Volume 172, Issue 2, pp 109–121 | Cite as

Hormonal stimulation of intestinal brush border enzymes release

  • Rosy Eloy
  • Jean Paul Vaultier
  • Francis Raul
  • Raouf Mirhom
  • Guy Clendinnen
  • Jacques F. Grenier
Article

Summary

The rat small bowel was perfused in vivo and ex vivo in the absence of biliary and pancreatic secretion. Intraluminal release of sucrase, alkaline phosphatase, aminopeptidases and enterokinase was significantly increased after administration of pentagastrin, caerulein and glucagon at doses ranging between 1 pg and 10 µg. This suggests that there is a direct hormonal stimulation of the intestinal mucosa. This effect might at least partly be mediated through cyclic AMP since dibutyryl derivates of this cyclic nucleotide exerted a significant stimulatory effect on intraluminal release of proteins, sucrase and enterokinase, although the pattern of enzyme was quite different from the effect produced by the three peptides.

Key words

Brush border Enzymes Intestine Hormones Glucagon Pentagastrin Caerulein DBcAMP 

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References

  1. 1.
    Alpers, D. H., Tedesco, F. Y.: The role of pancreatic proteases in the turnover of intestinal brush border proteins. Biochim. Biophys. Acta401, 28–40 (1975)PubMedGoogle Scholar
  2. 2.
    Baratti, J., Maroux, S., Louvard, D.: Effect of ionic strength and calcium on the activation of trypsinogen by enterokinase. Biochim. Biophys. Acta321, 632–638 (1973)PubMedGoogle Scholar
  3. 3.
    Barbezat, G. O., Grossman, M. I.: Intestinal secretion: stimulation by peptides. Science174, 422–424 (1971)PubMedGoogle Scholar
  4. 4.
    Dahlqvist, A.: Method for assay of intestinal disaccharidases. Anal. Biochem.7, 18–25 (1964)Google Scholar
  5. 5.
    Dahlqvist, A., Borgström, B.: Digestion and absorption of disaccharides in man. Biochem. J.81, 411–418 (1961)PubMedGoogle Scholar
  6. 6.
    Dyck, W. P., Bonnet, D., Lasater, G., Stinson, C., Hall, F. F.: Hormonal stimulation of intestinal disaccharidase release in the dog. Gastroenterology66, 533–538 (1974)PubMedGoogle Scholar
  7. 7.
    Dyck, W. P., Hall, F. F., Ratliff, C. R.: Hormonal control of intestinal alkaline phosphatase secretion in the dog. Gastroenterology65, 445–450 (1973)PubMedGoogle Scholar
  8. 8.
    Dyck, W. P., Martin, G. A., Ratliff, C. R.: Influence of secretin and cholecystokinin on intestinal alkaline phosphatase secretion. Gastroenterology64, 599–602 (1973)PubMedGoogle Scholar
  9. 9.
    Eloy, R., Haffen, K., Grenier, J. F.: Enzymes in the normal and diseased small intestinal mucosa: Development, localization, secretion and control. In: Progress in gastroenterology (Jerzy Glass, ed.). New York: Grune and Straton 1977Google Scholar
  10. 10.
    Eloy, R., Raul, F., Pousse, A., Mirhom, R., Ananna, A., Grenier, J. F.: Ex vivo vascular perfusion of the isolated rat small bowel. Importance of the intestinal brush border enzymerelease in basal conditions. Eur. Surg. Res.9, 96–112 (1977)PubMedGoogle Scholar
  11. 11.
    Goldman, R. B., Kim, Y. S., Jones, R. S., Sleisenger, M. H.: The effect of glucagon on enterokinase secretion from Brunner's gland pouches in dogs. Proc. Soc. Exp. Biol. (N.Y.)138, 562–565 (1971)Google Scholar
  12. 12.
    Götze, H., Adelson, J. B., Hadorn, B.: Hormone-elicited enzyme release by the small intestinal wall. Gut13, 471–476 (1972)PubMedGoogle Scholar
  13. 13.
    Johnson, L. R., Lichtenberger, L. M., Copeland, E. M., Dudrick, S. J., Castro, G. A.: Action of gastrin on gastrointestinal structure and function. Gastroenterology68, 1184–1192 (1975)PubMedGoogle Scholar
  14. 14.
    Joyner, W. L., Kokas, E.: Effect of various gastrointestinal hormones and vasoactive substances on villous motility. Comp. Biochem. Physiol.46A, 171–181 (1973)Google Scholar
  15. 15.
    Leblond, C. P., Carrière, R.: The effect of growth hormone and thyroxine on the mitotic rate of the intestinal mucosa of the rat. Endocrinology56, 261–266 (1955)PubMedGoogle Scholar
  16. 16.
    Levin, R. J.: The effects of hormones on the absorptive metabolic and digestive functions of the small intestine. J. Endocr.45, 315–348 (1969)PubMedGoogle Scholar
  17. 17.
    Louvard, D., Maroux, S., Baratti, J., Desnuelle, P., Mutaftschiev, S.: On the preparation and some properties of closed membrane vesicles from hog duodenal and jejunal brush border. Biochim. Biophys. Acta291, 747–763 (1973)PubMedGoogle Scholar
  18. 18.
    Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–275 (1951)PubMedGoogle Scholar
  19. 19.
    Magee, D. F.: The secretion of the small intestine. In his Gastro-intestinal Physiology. Springfield (Ill.): Thomas 1962Google Scholar
  20. 20.
    Maroux, S., Louvard, D., Baratti, J.: The aminopeptidase from hog intestinal brush border. Biochim. Biophys. Acta321, 282–295 (1973)PubMedGoogle Scholar
  21. 21.
    Nasset, E. S.: Enterocrinin, a hormone which excites the glands of the small intestine. Am. J. Physiol.121, 481–487 (1938)Google Scholar
  22. 22.
    Nasset, E. S.: Enterocrinin. In: Candidate hormones of the gut (Grossman, M. I. and others), pp. 744–745. Gastroenterology67, 730–755 (1974)PubMedGoogle Scholar
  23. 23.
    Nasset, E. S., Ju, J. S.: Stimulation of intestinal enzyme secretion by cholecystokininpancreozymin (CCK-PZ). Proc. Int. Union Physiol. Sci.9, 415 (1971)Google Scholar
  24. 24.
    Nasset, E. S., Pierce, H. B., Murlin, J. R.: Proof of a humoral control of intestinal secretion. Am. J. Physiol.111, 145–158 (1935)Google Scholar
  25. 25.
    Nordstrom, C.: Release of enteropeptidase and other brush border enzymes from the small intestinal wall in the rat. Biochim. Biophys. Acta289, 367–377 (1972)PubMedGoogle Scholar
  26. 26.
    Rommel, K., Böhmer, R.: Influence of secretin and cholecystokinin on maltase, sucrase and lactase in the jejunum of the rat. Digestion11, 194–198 (1974)PubMedGoogle Scholar
  27. 27.
    Rutgeerts, L., Tytgat, G., Eggermont, E.: Glucagon induced increase of enterokinase activity in human duodenal mucosa. Digestion8, 389–395 (1973)PubMedGoogle Scholar
  28. 28.
    Sutherland, E. W., Rall, T. W.: The relation of adenosine 3′,5′-phosphate and phosphorylase to the actions of catecholamines and other hormones. Pharmacol. Rev.12, 265–299 (1960)Google Scholar
  29. 29.
    Warnes, T. W., Hine, P., Kay, G.: Alkaline phosphatase in duodenal juice following secretin and pancreozymin. Gut10, 1049 (1969)Google Scholar
  30. 30.
    Warnes, T. W., Hine, P., Kay, G.: The action of secretin and pancreozymin on small intestinal alkaline phosphatase. Gut15, 39–47 (1974)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Rosy Eloy
    • 1
  • Jean Paul Vaultier
    • 1
  • Francis Raul
    • 1
  • Raouf Mirhom
    • 1
  • Guy Clendinnen
    • 1
  • Jacques F. Grenier
    • 1
  1. 1.Unit 61 INSERMSurgical Research and Biophysiopathology of the IntestineStrasbourgFrance

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