Summary
Twenty days after the onset of alloxan-induced diabetes, a villous hyperplasia has developed in the intestines of rats having free access to food. The transformation is characterised by a considerable increase in the area of the villous surface, caused by an enhanced mitotic activity in the crypts. The absorption of glucose or methionine by jejunal loops, whether expressed in terms of serosal area or villous area, is unchanged at this stage. On the other hand, the specific activity of certain disaccharidases and dipeptidases in crude mucosal homogenates is greater in diabetic animals, but quantitative histochemistry revealed no changes in the activities of alkaline phosphatase, leucine amino-peptidase and non-specific esterase in the individual enterocytes. Thus the biochemical changes may simply reflect the hyperplasia of the mucosa. The blood sugar level does not appear to be directly responsible for the mucosal transformation; however, the positive correlation between the daily food intake and the villus height suggests a role of hyperphagia and consequent increased luminal nutrition in the development of the hyperplasia.
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SCHEDL, H.P., WILSON, H.D.: Effects of diabetes on intestinal growth and hexose transport in the rat. Am. J. Physiol.220, 1739–1745 (1971)
CASPARY, W.: Effect of insulin and experimental diabetes mellitus on the digestive-absorptive function of the small intestine. Digestion9, 248–263 (1973)
OLSEN, W., ROGERS, L.: Jejunal sucrase activity in diabetic rats. J. lab. clin. Med.77, 838–842 (1971)
FRERICHS, H., CREUTZFELDT, W.: Diabetes durch ß-Zytotoxine.In: Handbuch des Diabetes mellitus. Ernst Pfeifer Bd. I, p. 809–840. München: Lehmanns Verlag 1969
KERSHAW, T.G., NEAME, K.D., WISEMAN, G.: The effect of semi-starvation on absorption by rat small intestine in vitro and in vivo. J. Physiol. (Lond.)152, 182–190 (1960)
MÜLLER, W.A., FALOONA, G.R., UNGER, R.H.: The effect of experimental insulin deficiency on glucagon secretion. J. clin. Invest.50, 1992–1999 (1971)
OLSEN, W., ROSENBERG, I.H.: Intestinal transport of sugars and amino acids in diabetic rats. J. clin. Invest.49, 96–105 (1970)
JERVIS, E.L., LEVIN, R.J.: Anatomic adaptation of the alimentary tract of the rat to the hyperphagia of chronic alloxan-diabetes. Nature (Lond.)210, 391–393 (1966)
LORENZ-MEYER, H., GOTTESBÜREN, H., MENGE, H.et al.: Intestinal structure and function in relation to blood sugar levels and food intake in experimental diabetes.In: Intestinal Adaptation. Edited by R.H. Dowling, E.O. Riecken. p. 189–191. Stuttgart/New York: Schattauer Verlag 1974
LORENZ-MEYER, H., KÖHN, R., RIECKEN, E.O.: Vergleich verschiedener morphometrischer Methoden zur Verfassung der Schleimhautoberfläche des Rattendünndarms und deren Beziehung zur Funktion. Histochemistry49, 123–129 (1976)
SCHMIDT, F.H.: Die enzymatische Bestimmung der Glucose und Fruktose nebeneinander. Klin. Wschr.39, 1244–1247 (1961)
AURICCHIO, S., PIERRO, M., ORSATTI, M.: Assay of peptidase activities of intestinal brush border with L-amino-acid oxidase. Analyt. Biochem.39, 15–23 (1971)
MILLER, D.L., SCHEDL, H.P.: Nonabsorbed indicators: A comparison of phenol red and inulin-C14 and effect of perfusion technique. Gastroenterology62, 48–55 (1972)
DAHLQVIST, A.: A method for assay of intestinal disaccharidases. Analyt. Biochem.7, 18–25 (1964)
LOWRY, O.H., ROSEBROUGH, N.J., FARR, A.L.et al.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1961)
PEARSE, A.G.E.: Histochemistry, theoretical and applied, 2nd ed. London: Churchill 1960
CLARKE, R.M.: Mucosal architecture and epithelial cell production rate in the small intestine of the albino rat. J. Anat.107, 519–529 (1970)
Wissenschaftliche Tabellen. Documenta Geigy. 7. Aufl. Basel: Geigy AG. Pharma 1968
NAKABOU, Y., OKITA, C., TAKANO, Y.,et al.: Hyperplastic and hypertrophic changes of the small intestine in alloxan diabetic rats. J. Nutr. Sci. Vitaminol.20, 227–234 (1974)
RIECKEN, E.O.: Die normale Dünndarmschleimhaut und ihre Veränderungen bei der einheimischen Sprue. Dtsch. Med. Wschr.95, 2295–2303 (1970)
MENGE, H., WERNER, H., LORENZ-MEYER, H.et al.: The nutritive effect of glucose on the structure and function of jejunal self-emptying blind loops in the rat. Gut16, 462–472 (1975)
GALJAARD, H., BOOTSMA, D.: The regulation of cell proliferation and differentiation in the intestinal epithelium. Exp. Cell. Res.58, 79–92 (1969)
CLARKE, R.M.: The effect of growth and of fasting on the number of villi and crypts in the small intestine of the albino rat. J. Anat.122, 27–33 (1972)
MENGE, H., ROBINSON, J.W.L., RIECKEN, E.O.: Anpassungsmöglichkeiten der Dünndarmschleimhaut an verschiedene intralumanle Milieuveränderungen. Z. Gastroent.14, 420–433 (1976)
ROSENZWEIG, N.S., HERMANN, R.H.: Control of jejunal sucrase and maltase activity by dietary sucrose and fructose in man. J. clin. Invest.47, 2253–2262 (1968)
MENGE, H., GRÄFE, M., LORENZ-MEYER, H.et al.: The influence of food intake on the development of structural and functional adaptation following ileal resection in the rat. Gut16, 468–472 (1975)
FÁBRY, P., KUJALOVÁ, V., PETRÁSEK, R.: Einige Folgeerscheinungen der funktionellen und morphologischen Adaptation an geänderte Kalorienzufuhr. Nahrung3, 642–649 (1959)
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Lorenz-Meyer, H., Thiel, F., Menge, H. et al. Structural and functional studies on the transformation of the intestinal mucosa in rats with experimental diabetes. Res. Exp. Med. 170, 89–99 (1977). https://doi.org/10.1007/BF01851379
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DOI: https://doi.org/10.1007/BF01851379