Summary
Intravenous administration of 1 U cholecystokinin-pancreozymin (CCK-PZ) to rats caused the release of enteropeptidase, alkaline phosphatase (AP), and sucrase to the intestinal lumen in the absence of a concomitant increase in luminal DNA. Thus, the hormone elicited hydrolase secretion was not due to cell desquamation. Pentagastrin also stimulated hydrolase release. Following CCK-PZ administration enteropeptidase was released preferentially over sucrase and AP and showed a linear correlation with total protein output. The specific enteropeptidase activity was higher in the perfusate following secretion than in the mucosa. Enteropeptidase was found mainly in soluble form in both mucosa and perfusate; addition of bile following enteropeptidase release further increased its activity. In contrast, sucrase and AP were found mainly in insoluble form in both mucosa and perfusate and their specific activities were higher in the mucosa. The presence of bile rendered both sucrase and AP more soluble in the perfusate. The data indicate that enteropeptidase is released by a specific secretory process and that its subcellular site of origin is different from that of sucrase and AP. By eliciting the coordinated release of trypsinogen, enteropeptidase and bile, CCK-PZ plays a central role in the initiation of protein digestion.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Nasset, E. S., Ju, J. S.: Stimulation of intestinal enzyme secretion by cholecystokininpancreozymin. Proc. Int. Union Physiol. Sciences9, 415 (1971)
Götze, H., Adelson, J. W., Hadorn, H. B., et al.: Hormone-elicited enzyme release by the small intestinal wall. Gut13, 471–476 (1972)
Moss, S., Lobley, R. W., Holmes, R.: Enterokinase in human duodenal juice following secretin and pancreozymin. Arch. Fr. Mal. App. Dig.61, 214c (1972)
Dyck, W. P., Hall, F. F., Rattliff, C. R.: Hormonal control of alkaline phosphatase secretion in dog. Gastroenterology65, 445–450 (1973)
Warnes, T. W., Hine, P., Kay, G.: The action of secretin and pancreozymin on small intestinal phosphatase. Gut15, 39–47 (1974)
Dyck, W.P., Bonnet, D., Lasater, J., et al.: Hormonal stimulation of intestinal disaccharidase release in the dog. Gastroenterology66, 533–538 (1974)
Goldman, R. B., Kim, Y. S., Jones, R. S., et al.: The effect of glucagon on enterokinase secretion from Brunner's gland pouches in dogs. Proc. Soc. Exp. Biol. Med.138, 562–565 (1971)
Fara, J. W., Campbell, R.: Intestinal vascular and secretory responses to gastrointestinal hormones. Fed. Proc.35, 449 (1976)
Croft, D. N., Loehry, C. A., Taylor, J. F. N., et al.: DNA and cell loss from normal small intestinal mucosa. Lancet1968 II, 70–73
Rinderknecht, H., Engeling, E. R., Bunnell, M. J., et al.: A sensitive assay for human enterokinase and some properties of the enzyme. Clin. Chim. Acta54, 145–160 (1974)
Hofstee, B. H. J.: Direct and continuous spectrophotometric assay of phosphomonoesterase. Arch. Biochem.51, 139–146 (1954)
Messer, M., Dahlquist, A.: One-step ultramicro-method for the assay of intestinal disaccharidases. Anal. Biochem.14, 376–392 (1966)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., et al.: Protein measurement with the folin phenol reagent. J. Biol. Chem.193, 265–275 (1951)
Layne, E.: Spectrophotometric and turbidometric methods for measuring proteins. In: Methods of Enzymology, S. P. Colowich, N. O. Kaplan, eds., Vol. III, pp. 446–453. 1957
Halliburton, I. W., Thomson, R. Y.: Chemical aspects of compensatory renal hypertrophy. Cancer Res.25, 1882–1887 (1965)
Cerotti, G.: A microchemical determination of desoxyribonucleic acid. J. Biol. Chem.198, 297–303 (1952)
Alpers, D. H., Tedesco, F. J.: The possible role of pancreatic proteases in the turnover of intestinal brush-border proteins. Biochim. Biophys. Acta401, 28–40 (1975)
Steiner, N., Hadorn, H. B., Sumida, C., et al.: Solubilization and activation of intestinal enterokinase by bile salts. Acta Paediatr. Scand.61, 499 (1972)
Hadorn, H. B., Hess, J., Troesch, V., et al.: Role of bile acids in the activation of trypsinogen by enterokinase: disturbance of trypsinogen activation in patients with intrahepatic biliary atresia. Gastroenterology66, 548–555 (1974)
Nordström, C.: Release of enteropeptidase and other brush-border enzymes from the small intestinal wall in the rat. Biochim. Biophys. Acta289, 367–377 (1972)
Louvard, D., Maroux, S., Baratti, J., et al.: On the distribution of enterokinase and on its subcellular localization. Biochim. Biophys. Acta309, 127–137 (1973)
Holmes, R., Lobley, W. R.: Localization of enterokinase to the brush-border membrane of the guinea-pig small intestine. J. Physiol.211, 50–51P (1970)
Nordström, C., Dahlquist, A.: The cellular localization of enterokinase. Biochem. Biophys. Acta198, 621–622 (1970)
Lebenthal, E., Morrissey, G. W.: Subcellular localization of enterokinase in rat small intestine. Gastroenterology70, 906 (1976)
Woodley, J. F., Keane, R.: Enterokinase in normal intestinal biopsies and those from patients with untreated celiac disease. Gut13, 900–902 (1972)
Nasset, E. S.: Enterocrinin, a hormone which excites the glands of the small intestine. Am. J. Physiol.121, 481–487 (1938)
Linderstrøm-Lang, K. U., Ottesen, M.: Formation of plakalbumin from ovalbumin. Comp. Rend. Trav. Lab. Carlsberg26, 403–410 (1952)
Author information
Authors and Affiliations
Additional information
Supported in part by U.S.P.H.S. Grants AM 17942 and AM 19973, the American-Swiss Foundation for Scientific Exchange, and the Swiss National Funds
Rights and permissions
About this article
Cite this article
Götze, H., Götze, J. & Adelson, J.W. Studies on intestinal enzyme secretion: The action of cholecystokinin-pancreozymin, pentagastrin and bile. Res. Exp. Med. 173, 17–25 (1978). https://doi.org/10.1007/BF01851370
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01851370