Research in Experimental Medicine

, Volume 168, Issue 3, pp 187–198 | Cite as

Influence of retrograde volume and short time cholestasis on the biliary reabsorption of sulfobromophthalein sodium (BSP) and phenol 3,6 dibromphthalein disulfonate (DBSP) from the rat biliary tree after retrograde intrabiliary injection (RII)

  • Hanns G. Dammann
  • Joachim v. Essen
Article

Summary

The retrograde intrabiliary injection (RII) technique was used to study the reabsorption of equimolar doses (1,2 µmol) of sulfobromopthalein sodium (BSP) and its analogue phenol 3,6 dibromphthalein disulfonate (DBSP) from the rat biliary tree under the experimental conditions established in this study i.e. variation of retrograde volumes injected and of short time cholestasis. RII was performed with a microliter syringe joined to a cannula positioned in the proximal third of the common bile duct. This technique guaranteed accurate administration of microliter quantities, also free bile flow could be reinitiated 3 – 5 sec after RII. Bile samples were collected in 2,5 min intervals up to the 5th min, then in 5 min intervals up to the 15th min, and the following 15 min up to the 30th min and analysed for compounds administered by RII. No significant differences in the biliary reabsorption of BSP and DBSP after RII could be detected. Increasing retrograde volumes of 20, 40, 60, 110, and 160 µ1 lead to decreasing percent recoveries in the bile in the first 2,5 min after RII (88,0 ± 3,8 − 15,6 ± 3,1) when bile flow was started at once after RII. An inverse correlation between retrograde volume and percent recoveries was found. Increasing duration of cholestasis (bile flow was started at once, 0,5 min, 3 min, 6 min, and 30 min after RII) results in decreasing percent recoveries of BSP and DBSP in the first 2,5 min after free bile flow was reinitiated (52,7 ± 3,6 − 20,0 ± 1,2). A time dependent proportional effect could be detected. Furthermore in the case of BSP concomitant with increasing cholestasis increasing amounts of BSP -conjugates were found in the bile supporting the idea of a continuing ductular- hepatocellular circulation even during complete obstruction of the common bile duct.

Key words

Biliary reabsorption retrograde intrabiliary injection sulfobromophthalein sodium and phenol 3,6 dibromphthalein disulfonate retrograde volume short time cholestasis 

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References

  1. 1.
    BARBER-RILEY, G.: Measurement of capacity of biliary tree in rats. Am. J. Physiol.205, 1122 - 1126 (1963a)PubMedGoogle Scholar
  2. 2.
    BARBER-RILEY, G.: Rat biliary tree during short periods of obstruction of common duct. Am.J. Physiol.205, 1127–1131 (1963b)PubMedGoogle Scholar
  3. 3.
    CLARK, A.G., HIROM, P.C., MILLBURN, P., SMITH, R.L.: Absorption of some organic compounds from the biliary system of the rat. J. Pharm. Pharmac.23, 150–152 (1971)Google Scholar
  4. 4.
    CZOK, G., DAMMANN, H.H.: Evidence of absorption of Bromsulphthalein from the biliary system of the rat. J. Pharm. Pharmac.24, 820–821Google Scholar
  5. 5.
    DAMMANN, H.G., CZOK, G.: Differences of reabsorption of unconjugated BSP and BSP - glutathione from the rat biliary tree after retrograde intrabiliary injection. Res. exp. Med.165, 181–190 (1975)Google Scholar
  6. 6.
    DAMMANN, H.G., SÜSSENBACH, J., CZOK, G.: Influence of dehydrocholate sodium on the biliary reabsorption of sulfobromopthalein sodium from the biliary tree of the rat after retrograde intrabiliary injection. Klin. Wschr.54, 789–791 (1976)PubMedGoogle Scholar
  7. 7.
    FISHER, B., VARS, H.M.: A method of collecting bile in rats; normal values on rat bile. Amer. J. Med. Sci.222, 116 (1951)Google Scholar
  8. 8.
    FUJIMOTO, J.M.: A retrograde push back technique (RPB) for estimating distended biliary tree capacity in rats. Fed. Proc.34, A 2784 (1975)Google Scholar
  9. 9.
    GUSTAFSON, J.H., BENET, L.Z.: Biliary excretion kinetic of phenolphthalein glucuronide after intravenous and retrograde biliary administration. J. Pharm. Pharmac.26, 937–944 (1974)Google Scholar
  10. 10.
    HAMPTON, J.C.: An electron microscope study of the hepatic uptake and excretion of submicroscopic particles injected into the blood stream and into the bile duct. Acta Anat.32, 262 (1958)PubMedGoogle Scholar
  11. 11.
    HOCH, J., COMBES, B.: Biliary excretion of pheno13,6 dibromphthalein disulfonate in rats fed a protein - free diet. Proc. Soc. Exptl. Biol. Med.127, 789–792Google Scholar
  12. 12.
    JAVITT, N.B.: Pheno13,6dibromsulphthalein, a new compound for the study of the liver disease. Proc. Soc. Exptl. Biol. Med.117, 254–257 (1964)Google Scholar
  13. 13.
    PETERSON, R.E., FUJIMOTO, J.M.: Retrograde intrabiliary injection. Absorption of water and other compounds from the rat biliary tree. J. Pharmacol. Exp. Ther.185, 150–162 (1973)PubMedGoogle Scholar
  14. 14.
    PETERSON, R.E., FUJIMOTO, J.M.: A study of absorption of compounds from the rat biliary tree by retrograde intrabiliary injection (RII). J. Pharmacol. Exp. Ther.194, 126–134 (1975)PubMedGoogle Scholar
  15. 15.
    ROBERTS, R.J., KLAASSEN, C.D., PLAA, G.L.: Maximum biliary excretion of bilirubin and sulfobromophthalein during anaesthesia - induced alteration of rectal temperature. Proc. Soc. Exptl. Biol. Med.125, 313–316 (1967)Google Scholar
  16. 16.
    SCHAFFNER, F.: Morphologic studies on bile secretion. Amer. J. Dig. Dis.10, 99–115 (1965)PubMedGoogle Scholar
  17. 17.
    STEINER, J.W., CARRUTHERS, J.S., KALFAIT, S.R.: Disturbances of hydration of cells of rat liver in extrahepatic cholestasis. Virchow Arch. Path. Anat.336, 99 (1962)PubMedGoogle Scholar
  18. 18.
    TANIKAWA, K.: Ultrastructural aspects of the liver and its disorders, pp. 110, New York: Springer-Verlag, 1968Google Scholar
  19. 19.
    WHELAN, G., HOCH, J., COMBES, B.: A direct assessment of the importance of conjugation for biliary transport of sulfobromophthalein sodium. J. Lab. Clin. Med.75, 542–547 (1970)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Hanns G. Dammann
    • 1
  • Joachim v. Essen
  1. 1.I. Medizinische KlinikUniversität HamburgHamburg 20Federal Republic of Germany

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