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A simple and efficient method for preparation of immunoelectrophoretically pure guinea pig IgM and isolation of monospecific anti-IgM antibodies

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Research in Experimental Medicine

Summary

This report describes experimental conditions developed for the rapid and efficient preparation of guinea pig IgM in a purified form and in high yield. A combined immunization procedure had to be designed to obtain sufficiently high IgM concentrations in guinea pig serum. The isolation procedure includes precipitation at low pH, followed by sedimentation at 110,000 g and reverse flow gel chromatography on Sephadex G-200. Furthermore, we describe in detail the isolation of relatively large amounts of pure and specific anti-guinea pig μ-chain antibodies by immunoaffinity chromatography.

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Abbreviations

FCA:

Freund's complete adjuvant

i.m.:

intramuscular

DEAE:

diethylaminoethyl-substituted cellulose

IEP:

immunoelectrophoresis

RIA:

radioimmunoassay

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Authors and Affiliations

  1. Medizinische Universitätsklinik (Innere Medizin I), Universität des Saarlandes, D-6650, Homburg, Federal Republic of Germany

    Harald Mauch

  2. Abteilung für Virologie des Institutes für Hygiene und Mikrobiologie, Universität des Saarlandes, D-6650, Homburg, Federal Republic of Germany

    Gunther Kümel

Authors
  1. Harald Mauch
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  2. Gunther Kümel
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Additional information

This work was supported by grants of the Deutsche Forschungsgemeinschaft and the BMFT of the FRG

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Mauch, H., Kümel, G. A simple and efficient method for preparation of immunoelectrophoretically pure guinea pig IgM and isolation of monospecific anti-IgM antibodies. Res. Exp. Med. 175, 279–286 (1979). https://doi.org/10.1007/BF01851284

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  • DOI: https://doi.org/10.1007/BF01851284

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