Summary
To study the extent, duration, and localization of metabolic changes in vein-to-vein grafts in rats, the sequential changes in enzymatic activity of veno-venous grafts in rats were evaluated by histochemical demonstration of the activity of two oxidoreductases (lactate dehydrogenase and succinate dehydrogenase) and two hydrolytic enzymes (adenosine triphosphatase and alkaline phosphatase).
All the enzymes studied showed a decrease in staining 1 day after transplantation, the change being most pronounced for AFOS in the subendothelial layer. The recovery of staining intensity was noted after 3 days, the enzyme activity reaching the pregrafting level at 1 week.
Different from the previous observations on vein-to-artery grafts, these vein-to-vein grafts showed less depression of enzyme activity during the first days after transplantation, neither did they display a continuing strong activity later on, probably due to lack of a thickening intimal layer.
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References
Balogh K, Jr, Dudley HR, Cohen RB (1961) Oxidative enzyme activity in skeletal cartilage and bone. A histochemical study. Lab Invest 10:839–845
Dunphy JE (1959) In: Patterson WB (ed) Wound healing and tissue repair. The University of Chicago Press, Chicago, p 55
Nachlas MM, Tsou K-C, de Souza E, Cheng C-S, Seligman AM (1957) Cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole. J Histochem Cytochem 5:420–436
Pearse Everson AG (1968) In: Histochemistry theoretical and applied, vol 1, 3rd edn. Churchill, London, p 720
Pearse Everson AG (1968) In: Histochemistry theoretical and applied, vol 1, 3rd edn. Churchill, London, p 710
Raekallio J (1970) Enzyme histochemistry of wound healing. Progr Histochem Cytochem 1:51–152
Raekallio J (1973) Estimation of the age of injuries by histochemical and biochemical methods. Z Rechtsmed 73:83–102
Remes V, von Smitten K, Waris T, Raekallio J (1986) Histochemical examination of energy metabolism in aortic vein grafts in rats. Cardiovasc Res 20:783–788
Remes V, von Smitten K, Waris T, Raekallio J (1987) Assessment of metabolic activity in aortic vein grafts in rats by histochemical examination of hydrolases. Eur Surg Res [Suppl 1] 19:68–69 [Abstr]
von Smitten K, Stenman S, Scheinin TM (1980) Connective tissue changes in syngeneic aortal vein grafts in rats. Acta Chir Scand 146:253–260
Stoward PJ (1983) Substances identified by histochemical methods. In: Filipe MI, Lake BD (eds) Histochemistry in pathology. Churchill and Livingstone, New York, pp 12–25
Welsh P, Parisi C, Rosas G, Repetto R, Paladino C (1975) Degenerative changes in autologous saphenous veins used as arterial bypass grafts. J Cardiovasc Surg 15:700–705
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Supported by grants from the Paolo Foundation, Finland
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Remes, V. Enzyme histochemical studies on veno-venous grafts in rats. Res. Exp. Med. 189, 121–127 (1989). https://doi.org/10.1007/BF01851262
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DOI: https://doi.org/10.1007/BF01851262