Summary
164 inbred Wistar rats received subcutaneous and intraperitoneal implants of xenogeneic collagen sponges and were examined by light- and electron microscopy as well as immunohistologically, to establish tissue and cell reactions and the time of collagen reabsorption. 4 to 5 weeks after implantation the xenogeneic collagen sponge was completely absorbed.
All the inflammatory and reparative reactions were non-specific. Granulation tissue appeared soon (3 days after implantation) with new synthesis of fine collagen fibrils, mainly of type III, followed by type I collagen fibers.
Myofibroblasts, with intracellular evidence of myosin and collagen (Type I and III) were sporadically present in the granulation tissue after day 5 and regularly after day 10. Small scar granulomas without xenogenic collagen were detectable even 4 to 6 months after collagen implantation. Reabsorption and regression of collagen granulomas occurred faster after xenogeneic collagen implantation than after using surgical cat sutures.
From the morphological point of view, there is no indication of specific sensitization against xenogeneic collagen or of a specific immunologic process.
Zusammenfassung
An 164 Inzuchtratten wurden morphologische Gewebsreaktionen und Resorptionszeiten von implantiertem xenogenem Kollagenschaum untersucht:
Die entzündlich-resorptiven Veränderungen im Gewebe sind unspezifisch. Die Granulationsgewebsbildung tritt rasch auf. Das xenogene Kollagen ist nach 4 bis 5 Wochen vollständig resorbiert.
Kleine Narbengranulome finden sich zum Teil noch nach 5 bis 6 Monaten. Die Resorption und Rückbildung der Kollagenschaumgranulome erfolgt rascher als bei der Verwendung von chirurgischem Catgut. Für eine spezifische Sensibilisierung gegen das Fremdkollagen bzw. für den Ablauf spezifischer immunologischer Vorgänge ergibt sich morphologisch kein Anhalt.
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Literatur
Adelmann, B. C., Glynn, L. E., Kirrane, J.: DLH reactions of native and denatured rat and calf skin collagen in sensitive guinea pigs (Abstr.). Fed. Proc. Fed. Am. Socs. Exp. Biol.27, 263 (1968)
Balleisen, L., Gay, S., Marx, R., Kühn, K.: Comparative investigation on the influence of human bovine collagen Types I, II and III on the aggregation of human platelets. Klin. Wschr.53, 903–905 (1975)
Beard, H. K., Page Faulk, W., Conochie, L. B., Glynn, L. E.: Some immunological aspects of collagen. Prog. Allergy22, 45–106 (1977)
Bergqvist, D., Falk, J., Ståhl, A.: Tissue reaction to implantation of collagen film. Upsala J. Med. Sci.82, 203–208 (1977)
Gabbiani, G., Le Lous, M., Bailey, A. J., Bazin, S., Delaunay, A.: Collagen and myofibroblasts of granulation tissue. Virchows Arch. B Cell Pathol.21, 133–145 (1976)
Gay, S., Müller, P. K., Lemmen, C., Remberger, K., Matzen, K., Kühn, K.: Immunohistological study in cartilage-bone metamorphosis and degenerative osteoarthrosis. Klin. Wschr.54, 969–976 (1976)
Gröschel-Stewart, U., Schreiber, J., Mahlmeister, Ch., Weber, K.: Production of specific antibodies to contractile proteins, and their use in immunofluorescence microscopy. I. Antibodies to smooth and striated chicken muscle myosins. Histochemistry46, 229–236 (1976)
Harris, E. D., Krane, S. M.: Collagenases. N. Engl. J. Med.291, 557–563, 605–609, 652–661 (1974)
Hopper, K. E., Adelmann, B. C., Gentner, G., Gay, S.: Recognition by guinea-pig peritonealexudate cells of confirmationally different states of the collagen molecule. Immunology30, 249 (1976)
Horwitz, A. L., Hance, A. J., Crystal, R. G.: Granulocyte collagenase: Selective digestion of type I relative to type III collagen. Proc. Natl. Acad. Sci. USA74, 897–901 (1977)
Jaffé, R., Deykin, D.: Evidence for a structural requirement for the aggregation of platelets by collagen. J. Clin. Invest.53, 875 (1974)
Miller, E. J.: Biochemical characteristics and biological significance of the genetically distinct collagens. Mol. Cell. Biochem.13, 165–192 (1976)
Remberger, K., Gay, S., Adelmann, B. C.: Altersabhängige Unterschiede in Lokalisation und Verteilung von Kollagen Typ I, II und III in verschiedenen menschlichen Geweben. Verh. Dtsch. Ges. Pathol.59, 276–280 (1975)
Remberger, K., Gokel, J. M., Gay, S., Meister, P.: Myofibroblasten und Kollagentypen bei Fibromatosen. Verh. Dtsch. Ges. Pathol.61, 388 (1977)
Springorum, H. W., Adler, C. P., Jäger, W., Ober, E.: Tierexperimentelle Untersuchung der Knochenregeneration am standardisierten Tibiadefekt des Kaninchens nach Implantation von Kollagenvlies im Vergleich zur autologen und homologen Spongiosaplastik. Z. Orthop.115, 686–693 (1977)
Stöss, H., Pesch, H.-J.: Dura-Transplantation. Mehrzeitige Transplantationen von Lösungsmittel-getrockneter Dura mater. Tierexperimentelle Untersuchungen zur Frage der Sensibilisierung. Fortschr. Med.95, 1018–1021 (1977)
Timpl, R., Wick, G., Gay, S.: Antibodies to distinct types of collagens and procollagens and their application in immunohistology. J. Immunol. Methods18, 165–182 (1977)
Timpl, R.: In: Biochemistry of Collagen, G. N. Ramachandran, A. H. Reddi (eds.), p. 319. New York: Plenum Press 1976
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Remberger, K., Hübner, G. Experimentelle Untersuchungen über Zell- und Gewebsreaktionen nach Implantation von xenogenem Kollagenschaum. Res. Exp. Med. 175, 67–79 (1979). https://doi.org/10.1007/BF01851235
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DOI: https://doi.org/10.1007/BF01851235