Research in Experimental Medicine

, Volume 177, Issue 3, pp 255–261 | Cite as

Die Beeinflussung der in vitro-Befruchtungsrate der Maus durch Seren Spermatozoen-immunisierter Mäuse

  • L. Mettler
  • G. Ehrig
  • J. Volkmann
Original Contributions

Zusammenfassung

In der vorliegenden Arbeit sollte der Einfluß von „Spermatozoen-Antikörpern“ auf die in vitro-Fertilisationsrate der Maus untersucht werden. Dazu wurden Spermatozoen-Antikörper-enthaltende Seren, die wir durch 4wöchiges Immunisieren von Mäusen im syngenen und allogenen System gewannen, zu den in vitro-Kulturen gegeben. Die resultierenden Befruchtungsraten verglichen wir mit den Ergebnissen der in vitro-Kontrollkulturen. In den Vergleich einbezogen wurde auch die Zugabe der isolierten Gamma-Globulin-Fraktion aus Antiseren. Der Einfluß der „Spermatozoen-Antikörper“ zeigte sich in eindeutig erniedrigten Befruchtungsraten von 1–2% befruchteter Eizellen im Vergleich zu den normalerweise erreichten Befruchtungsraten von durchschnittlich 50%. Außerdem zeigten die Eizellen der Versuchsgruppe in allen Fällen eine Schrumpfung des Zellkörpers.

Schlüsselwörter

In vitro-Befruchtung Spermatozoen-Antikörper Rückgang der Befruchtungsrate 

Influence of sera from spermatozoa-immunized mice on the in vitro fertilization rate of the mouse

Summary

The present paper describes the influence of antibodies against spermatozoa on the in vitro fertilization of inbred mice. ‘Antibodies against spermatozoa’ were obtained by immunization in the syngeneic and allogeneic system of C57BL/J Han and C3H/2He female mice with washed spermatozoa of the corresponding male partners. These antibodies were added to the in vitro culture. The resulting fertilization rates were compared with control cultures where sera of non-immunized and erythrocytes sensitized mice were added. For comparison the same experiments were performed with isolated gammaglobulin fractions of the antisera. The influence of the antibodies against spermatozoa was demonstrated by the reduction of the cleavage rate (1–2% cleaved oocytes) in comparison to the normally achieved fertilization rate of about 50%. In addition, the oocytes of the experimental group revealed shrinking of the cell mass in all cases.

Key words

In vitro fertilization Antibodies against spermatozoa Reduction of cleavage rate 

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Literatur

  1. Behrman SJ, Nakayama M (1965) Antitestis antibody: its inhibition of pregnancy. Fert Steril 16:37–44Google Scholar
  2. Behrman SJ, Otani Y, Porter WC, Nakayama M (1963) Reduction of fertilita in immunized guinea pigs. Int J Fert 8:835–839Google Scholar
  3. Bell EB (1969) Iso-antibody formation against rabbit spermatozoa and its effect on fertility. J Reprod Fert 20:519–522Google Scholar
  4. Bell EB (1969) Immunological control of fertility in the mouse: a comparison of systematic and intravaginal immunization. J Reprod Fert 18:183–192Google Scholar
  5. Bell EB, McLaren A (1970) Reduction of fertility in female mice isoimmunized with a subcellular sperm fraction. J Reprod Fert 22:345–356Google Scholar
  6. Brock J (1976) Präparation von Immunglobulin. In: Immunologische Arbeitsmethoden, Bd 1. Fischer, Jena, S 207–274Google Scholar
  7. Edwards RG (1964) Immunological control of fertility in female mice. Nature 203:50–53PubMedGoogle Scholar
  8. Ehrig G, Mettler L (1977) Bedeutung der Kapazitation bei der in vivo- und in vitro-Befruchtung. In: Fortschritte der Fertilitätsforschung, Bd V. Grosse, Berlin, S 174–176Google Scholar
  9. Erickson RP (1973) Decreased fertility in female mice immunized with a carrier followed by small numbers of spermatozoa. J Reprod Fert 32:295–298Google Scholar
  10. Isojima S, Graham RM, Graham JB (1959) Sterility in female guinea pigs induced by injection with testis. Science 129:44PubMedGoogle Scholar
  11. Kaths S (1959) Infertility in female guinea pigs induced by injection of homologeous sperm. Am J Obstet Gynecol 78:276–278PubMedGoogle Scholar
  12. Landsteiner K (1899) Zur Kenntnis der spezifisch auf Blutkörperchen wirkenden Sera. Zentralbl Bakteriol [Orig A] 25:546–549Google Scholar
  13. Laurell CB (1965) Antigen-antibody crossed electrophoresis. Anal Biochem 10:358–361PubMedGoogle Scholar
  14. McLaren A (1964) Immunological control of fertility in female mice. Nature 201:582–585PubMedGoogle Scholar
  15. McLaren A (1966) Studies on the isoimmunization of mice with spermatozoa. Fert Steril 17:492–499Google Scholar
  16. Menge AC (1967) Induced infertility in cattle by isoimmunization with semen and testis. J Reprod Fert 13:445–456Google Scholar
  17. Menge AC (1968) Fertilization, embryo and fetal survival rate in rabbits isoimmunized with semen, testis, and conceptus. Proc Soc Exp Biol Med 127:1271–1275PubMedGoogle Scholar
  18. Menge AC (1969) Early embryo mortality in heifers isoimmunized with semen and conceptus. J Reprod Fert 18:67–74Google Scholar
  19. Menge AC, Liebermann ME (1974) Antifertility effects of immunglobulins from uterine fluids of semen-immunized rabbits. Biol Reprod 10:422–428PubMedGoogle Scholar
  20. Metalnikoff F (1900) Etudes sur la spermatoxine. Ann Inst Pasteur 14:577–589Google Scholar
  21. Metchnikoff E (1899) Etudes sur la resorption des cellules. Ann Inst Pasteur 13:737–770Google Scholar
  22. Mettler L (1977) Immunologische Aspekte der weiblichen Sterilität. Fortschritte der Fertilitätsforschung, IV. Grosse, BerlinGoogle Scholar
  23. O'Rand MG (1977) The presence of sperm-specific surface isoantigens on the egg following fertilization. J Exp Zool 202:267–273PubMedGoogle Scholar
  24. Mettler L, Gradl G, Shirvani D (1980) Occurrence of sperm antibodies in human reproduction, 1. Comparative new and improved test method for sperm antibody detection. Am J Obstet Gynecol 136:106–116PubMedGoogle Scholar
  25. Tsunoda Y, Chang MC (1976) Reproduction in rats and mice isoimmunized with homogenate of ovary or testis with epididymis or sperm suspensions. J Reprod Fert 46:379–382Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • L. Mettler
    • 1
  • G. Ehrig
    • 1
  • J. Volkmann
    • 1
  1. 1.Frauenheilkunde im Zentrum Operative Medizin I und Christian-Albrechts-Universität Kiel und Staatliche HebammenlehranstaltKielBundesrepublik Deutschland

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