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Research in Experimental Medicine

, Volume 188, Issue 5, pp 367–375 | Cite as

Establishment and characterization of a cell line from a human cholangiocellular carcinoma

  • Y. Kusaka
  • T. Tokiwa
  • J. Sato
Original Papers

Summary

A cell line, HuH-28, was established in vitro from a patient with cholangiocellular carcinoma (CCC). This cell line has grown slowly, revealing a doubling time of approximately 80 h, and the serial passages were carried out 20 times within 10 months. Light microscopy revealed spindle and polygonal morphology of the cells.

Chromosome number of the cells were distributed near the hypotriploid region at passages 3 and 14. HuH-28 cells were not transplantable into nude mice, but secreted some tumor markers including alkaline phosphatase (ALP), gamma glutamyltranspeptidase (GGT), β2-microglobulin (BMG), ferritin, elastase-1, and tissue polypeptide antigen (TPA). This HuH-28 cell line will represent a good model for the investigation of carcinogenesis, histogenes, and diagnosis of CCC.

Key words

Cell line Human (HuH-28) cells Cholangiocellular carcinoma 

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References

  1. 1.
    Aden DH, Fogel A, Plotkin S, Damjianov I, Knowles BB (1979) Controlled synthesis of HBs-Ag in a differentiated human liver carcinoma-derived cell line. Nature 282:615–616PubMedGoogle Scholar
  2. 2.
    Albert Z, Orlowska J, Orlowski M (1964) Histochemical and biochemical investigations of Gamma-glutamyl-transpeptidase in the tissues of man and laboratory rodents. Acta Histochem 18:78–89PubMedGoogle Scholar
  3. 3.
    Alexander JJ, Bey EM, Geddes EW, Lecatsas G (1976) Establishment of a continuously growing cell line from primary carcinoma of the liver. S Afr Med J 50:2124–2128PubMedGoogle Scholar
  4. 4.
    Burstone MS, Keyes PH (1957) The effect of inhibition of enzyme activity on developing bone and dentin. Am J Pathol 33:1229–1235PubMedGoogle Scholar
  5. 5.
    Chen JM, Chu TH, Yen HJ, Shen TW, Lu YH (1980) Establishment of three human liver carcinoma cell lines and some of their biological characteristics in vitro. Sci Sin 23:236–251PubMedGoogle Scholar
  6. 7.
    Doi I, Namba M, Sato J (1975) Establishment and some biological characteristics of human hepatoma cell lines. Gann 66:385–392PubMedGoogle Scholar
  7. 8.
    Doi I (1976) Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma. Gann 67:1–10PubMedGoogle Scholar
  8. 9.
    Foster JH (1970) Survival after liver resection for cancer. Cancer 26:493–502PubMedGoogle Scholar
  9. 10.
    He L, Isselbacher KJ, Wands JR, Goodman HM, Shin C, Quarori A (1984) Establishment and characterization of a new human hepatocellular carcinoma cell line. In Vitro 20:493–504PubMedGoogle Scholar
  10. 11.
    Huh N, Utakoji T (1981) Production of HBs-antigen by two new human hepatoma cell lines and its enhancement by dexamethasone. Gann 72:178–179PubMedGoogle Scholar
  11. 12.
    King EJ, Haslewood GAD, Delory GE, Beall D (1942) Micro-chemical methods of blood analysis. Lancet 1:207–209Google Scholar
  12. 13.
    Laishes BA, Ogawa K, Roberts E, Farber E (1978) Gamma-glutamyl transpeptidase: A positive marker for cultured rat liver cells derived from putative premalignant and malignant lesions. J Natl Cancer Inst 60:1009–1016PubMedGoogle Scholar
  13. 14.
    Miyagiwa M, Ichda T, Sasaki H, Tokiwa T, Sato J (1987) Establishment and characterization of human cholangiocellular carcinoma cell line (CI) producing high concentration of CA19/9. Abstr The Human Cell Res Soc 5:46–49 (in Japanese)Google Scholar
  14. 15.
    Murakami T (1984) Establishment and characterization of human hepatoma cell line (KIM-1). Acta Hepatol 25:1–8 (in Japanese)Google Scholar
  15. 16.
    Nakabayashi H, Taketa K, Miyano K, Yamane T, Sato J (1982) Growth of human hepatoma cell lines with differentiated function in chemically defined medium. Cancer Res 42:3858–3863PubMedGoogle Scholar
  16. 17.
    Rothfels KH, Siminovitch L (1958) An air-drying technique for flattening chromosomes in mammalian cells grown in vitro. Stain Technol 33:73–77PubMedGoogle Scholar
  17. 18.
    Schlegel R, Schlegel S-B, McLeod JA, Pinkus GS (1980) Immunoperoxidase localization of keratin in human neoplasms. Am J Pathol 101:41–49PubMedGoogle Scholar
  18. 19.
    Seabright MA (1971) A rapid banding technique for human chromosomes. Lancet 2:971–972Google Scholar
  19. 20.
    The Liver Cancer Study Group of Japan (1985) Survival and follow-up study of primary liver cancer in Japan. Acta Hepatol 26:254–262 (in Japanese)Google Scholar
  20. 21.
    Uotila M, Ruoslahti E, Engvall E (1981) Two site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J Immunol Methods 42:11–13PubMedGoogle Scholar
  21. 22.
    Yamaguchi N, Morioka H, Ohkura H, Hirohashi S, Kawai K (1985) Establishment and characterization of the human cholangiocarcinoma cell line HChol-Y1 in a serum-free, chemically defined medium. J Natl Cancer Inst 75:29–32PubMedGoogle Scholar
  22. 23.
    Yano H, Kojiro M, Nakashima T (1986) A new human hepatocellular carcinoma cell line (KYN-1) with a transformation to adenocarcinoma. In Vitro 22:637–646Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Y. Kusaka
    • 1
  • T. Tokiwa
    • 1
  • J. Sato
    • 1
  1. 1.Division of PathologyCancer Institute of Okayama University Medical SchoolOkayamaJapan

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