Summary
Chronic administration ofΔ 8- andΔ 9-tetrahydrocannabinols (4 mg/kg b.wt. i.p.) to sexually developing male rats every alternate day from age day 16 to age day 87 resulted in a significant decrease in pubertal growth spurts, especially the second peak. Endogenous tstosterone, dihydrotestosterone, LH and FSH concentration in blood declined significantly from those of the control animals. When the drugs were withdrawn at mid-puberty (age day 40) the “catch-up” phenomenon in body growth was evident although testosterone level continued to be suppressed but dihydrotestosterone level recovered. Withdrawal of the drugs resulted in better recovery in LH level compared to that of FSH. Administration of LH-RH to the animals receiving drugs until day 87 resulted in significantly (P < 0.001) lower levels of gonadotropins than those in the control animals. The inhibition inΔ 9-THC-treated animals was more severe than inΔ 8-THC-treated ones. When the drug was withdrawn,Δ 8-THC-treated animals responded to LH-RH stimulation better than did those treated withΔ 9-THC. These findings suggest that the tetra-hydrocannabinols perhaps mainly affect pituitary function.
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References
Mechoulam R (1973) Marihuana chemistry, pharmacology, metabolism, and clinical studies. Academic Press, New York
Herha J (1974) Haschisch and Marihuana — Eine Übersicht. Naturwissenschaften 61:70–74
Kalodny RC, Masters WH, Kolodner RM, Toro G (1974) Depression of plasma testosterone levels after chronic intensive marihuana use. N Engl J Med 20:872–874
Janzik HH, Kronert K, Gupta D (1975) Hormonal evaluation in young drug addicts. J Pharmacol Clin 2:60–62
Hembree WC III, Zeidenberg P, Nahas G (1976) In: Nahas G (ed) Marihuana: Chemistry, biochemistry and cellular effects. Springer, Berlin Heidelberg New York
Mendelson JH, Ellingboe J, Kuehnle J, Mello NK (1978) Effects of chronic marihuana use on integrated plasma testosterone and luteinizing hormone levels. J Pharmacol Exp Ther 207:611–617
Marks B (1972)Δ 1-Tetrahydrocannabinol and lutheinizing hormone secretion. Prog Brain Res 39:331–338
Dalterio S, Bartke A, Roberson C, Watson D, Burstein S (1978) Direct and pituitary mediated effects ofΔ 9-THC and cannabinol on the testis. Pharmacol Biochem Behav 8:673–678
Smith CG, Besch NF, Makita NJ (1978) Time course of the effects of tetrahydrocannabinols on testosterone levels in the male rhesus monkey. Proc 4th Annu Meet Am Soc Androl, Abst 54, p 35
Smith CG, Smith MT, Besch NF, Smith RG, Asch RH (1979) In: Nahas G, Paton WDM (eds) Marihuana: Biological effects. Analysis, metabolism, cellular responses, reproduction and brain, vol 22/23. Pergamon Press, Oxford
Chakravarty I, Shah PG, Sheth AR, Ghosh JJ (1979) Mode of action ofΔ 9-THC on hypothalamo-pituitary function in adult female rats. J Reprod Fert 57:113–115
Attanasio A, Gupta D (1975) Simultaneous radioimmunoassay of estrogens and androgens in plasma of prepubertal children. In: Gupta D (ed) Radioimmunoassay of steroid hormones. Verlag Chemie, Weinheim, pp 91–100
Gupta D, Attanasio A, Raaf S (1975) Plasma estrogen and androgen concentrations in children during adolescence. J Clin Endocrinol Metab 40:636–643
Greenwood FC, Hunter WM, Glover JS (1963) The preparation of131I-labelled human growth hormone of high specific radioactivity. Biochem J 89:114–123
Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11:1–42
Järbe TUC, Henriksson BG (1973) Acute effects of two tetrahydrocannabinols (Δ 9-THC andΔ 8-THC) on water intake in water deprived rats: Implications for behavioral studies on marijuana compounds. Psychopharmacologia 30:315–322
Sjöden PO, Järbe TUC, Henriksson BG (1973) Influence of tetrahydrocannabinols (Δ 8-THC andΔ 9-THC) one body weight, food and water intake in rats. Pharmacol Biochem Behav 1:395–399
Manning FG, McDonough JH Jr, Elsmore TF, Saller CH, Sodetz FJ (1971) Inhibition of normal growth by chronic administration ofΔ 9- tetrahydrocannabinol. Science 174:424–426
Dixit VP, Sharma NN, Lohiya NK (1974) The effect of chronically administered cannabis extract on the testicular function of mice. Euro J Pharmacol 26:111–114
Thomson GR, Mason MM, Rosenkranz H, Braude MC (1973) Chronic oral toxicity of cannabinoids in rats. Toxicol Appl Pharmacol 25:373–390
Purohit V, Singh HH, Ahluwalia BS (1979) Evidence that the effects of methadone and marihuana on male reproductive organs are mediated at different sites in rats. Biol Reprod 20:1039–1044
Harclerode J, Nyquist SE, Nazar B, Lowe D (1979) In: Nahas G, Paton WDM (eds) Marihuana: Biological effects. Analysis, metabolism, cellular responses, reproduction and brain, vol 22/23. Pergamon Press, Oxford
Zimmermann AM, Bruce WR, Zimmermann S (1979) Effects of cannabinoids on sperm morphology. Pharmacology 18:143–148
Collu R, Letarte J, Leboeuf G, Ducharme JR (1979) Endocrine effects of chronic administration of psychoactive drugs to prepubertal male rats. I:Δ 9-Tetrahydrocannabinol. Life Sci 16:533–542
Sofia RD, Dixit BN, Barry H III (1971) The effect ofΔ 1-tetrahydrocannabinol on serotonin metabolism in the rat brain. Life Sci 10:425–436
Truitt EB Jr, Anderson SH (1972) Biogenic amine alterations produced in the brain by tetrahydrocannabinols and their metabolites. Ann NY Acad Sci 191:68–71
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Gupta, D., Elbracht, C. Effect of tetrahydrocannabinols on pubertal body weight spurt and sex hormones in developing male rats. Res. Exp. Med. 182, 95–104 (1983). https://doi.org/10.1007/BF01851115
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DOI: https://doi.org/10.1007/BF01851115