Acta Neurochirurgica

, Volume 139, Issue 1, pp 77–81 | Cite as

Reduced expression of calponin in canine basilar artery after subarachnoid haemorrhage

  • M. Doi
  • H. Kasuya
  • B. Weir
  • D. A. Cook
  • A. Ogawa
Experimental Research

Summary

Calponin, an actin- and tropomyosin-binding protein, has been characterized as an inhibitory factor in the smooth-muscle actomyosin activity. The level of calponin was determined in canine basilar arteries in a double-haemorrhage model. Thirty dogs were assigned to three groups: day 0 group, control; day 2 group, dogs sacrificed 2 days after cisternal injection of blood; and day 7 group, dogs given double cisternal injections of blood and sacrificed 7 days after the first injection. Constriction of the basilar artery was confirmed by arterial angiography. Portions of the affected arteries or the corresponding region in control animals were solubilized for sodium dodecylsulphate-polyacrylamide gel electrophoresis and Western blotting. A major band corresponding to calponin was seen at 34 kD in the basilar artery extracts using chicken gizzard polyclonal antibodies. The densitometer values of the band on Coomassie blue-stained gels were expressed as percentages of day 0 control values. The signals of day 2 and day 7 samples were 47%±20% and 23%±12%, respectively (mean±standard deviation). The proportions of calponin to actin/tropomyosin in the day 0, day 2, and day 7 groups were 13%±6%, 6%±2%, and 4%±2%, respectively. The reduced expression of calponin may be related to sustained contraction during cerebral vasospasm.

Keywords

Calponin smooth muscle subarachnoid haemorrhage vasospasm 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Durand-Arczynska W, Marmy N, Durand J (1993) Caldesmon, calponin and α-smooth muscle actin expression in subcultured smooth muscle cells from human airways. Histochemistry 100: 465–471PubMedGoogle Scholar
  2. 2.
    Gimona M, Herzog M, Vandekerckhove J, Small JV (1990) Smooth muscle specific expression of calponin. FEBS Lett 274: 159–162PubMedGoogle Scholar
  3. 3.
    Hai CM, Murphy RA (1989) Ca2+, crossbridge phosphorylation, and contraction. Ann Rev Physiol 51: 285–298Google Scholar
  4. 4.
    Kasuya H, Weir BKA, Nakane M, Pollock JS, Johns L, Marton LS, Stefansson K (1995) Nitric oxide synthase and guanylate cyclase levels in canine basilar artery after subarachnoid hemorrhage. J Neurosurg 82: 250–255PubMedGoogle Scholar
  5. 5.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685PubMedGoogle Scholar
  6. 6.
    Minami N, Tani E, Maeda Y, Yamaura I, Nakano A (1993) Immunoblotting of contractile and cytoskeletal proteins of canine basilar artery in vasospasm. Neurosurgery 33: 698–706PubMedGoogle Scholar
  7. 7.
    Ngai PK, Walsh MP (1985) Detection of caldesmon in muscle and non-muscle tissues of the chicken using polyclonal antibodies. Biochem Biophys Res Commun 127: 533–539PubMedGoogle Scholar
  8. 8.
    Sakaki S, Ohue S, Kohno K, Takeda S (1989) Impairment of vascular reactivity and changes in intracellular calcium and calmodulin levels of smooth muscle cells in canine basilar arteries after subarachnoid hemorrhage. Neurosurgery 25: 753–761PubMedGoogle Scholar
  9. 9.
    Sasaki T, Kassell NF, Yamashita M, Fujiwara S, Zuccarello M (1985) Barrirer disruption in the major cerebral arteries following experimental subarachnoid hemorrhage. J Neurosurg 63: 433–440PubMedGoogle Scholar
  10. 10.
    Takahashi K, Hiwada K, Kokubu T (1986) Isolation and characterization of a 34000-dalton calmodulin- and F-actin-binding protein from chicken gizzard smooth muscle. Biochem Biophys Res Commun 141: 20–26PubMedGoogle Scholar
  11. 11.
    Takahashi K, Hiwada K, Kokubu T (1987) Occurrence of antigizzard p34K antibody cross reactive components in bovine smooth muscles and non-smooth muscle tissues. Life Sci 41: 291–296PubMedGoogle Scholar
  12. 12.
    Takahashi K, Hiwada K, Kokubu T (1988) Vascular smooth muscle calponin. A novel troponin T-like protein. Hypertension 11: 620–626PubMedGoogle Scholar
  13. 13.
    Takahashi K, Nadal-Ginard B (1991) Molecular cloning and sequence analysis of smooth muscle calponin. J Biol Chem 266: 13284–13288PubMedGoogle Scholar
  14. 14.
    Walsh MP (1994) Regulation of vascular smooth muscle tone. Can J Physiol Pharmacol 72: 919–936PubMedGoogle Scholar
  15. 15.
    Winder ST, Walsh MP (1990) Smooth muscle calponin. Inhibition of actomyosin MgATPase and regulation by phosphorylation. J Biol Chem 265: 10148–10155PubMedGoogle Scholar
  16. 16.
    Yokota M, Peterson JW, Kaoutzanis MC, Yamakawa K, Sibilia R, Zervas NT (1995) Protein kinase C and diacylglycerol content in basilar arteries during experimental cerebral vasospasm in the dog. J Neurosurg 82: 834–840PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • M. Doi
    • 1
  • H. Kasuya
    • 2
  • B. Weir
    • 4
  • D. A. Cook
    • 3
  • A. Ogawa
    • 1
  1. 1.Department of NeurosurgeryIwate Medical UniversityIwateJapan
  2. 2.Department of NeurosurgeryTokyo Women's Medical CollegeTokyoJapan
  3. 3.Department of PharmacologyUniversity of AlbertaEdmonton
  4. 4.Section of NeurosurgeryUniversity of ChicagoChicagoUSA

Personalised recommendations