The Histochemical Journal

, Volume 21, Issue 8, pp 455–460 | Cite as

Production and immunohistochemical characterization of a monoclonal antibody raised to proteoglycan purified from a human yolk sac tumour

  • Mitsuko Sobue
  • Nobuo Nakashima
  • Toshiaki Fukatsu
  • Tetsuro Nagasaka
  • Shinji Fukata
  • Noboru Ohiwa
  • Yoshiharu Nara
  • Takashi Ogura
  • Takashi Katoh
  • Jun Takeuchi
Papers

Summary

A large proteoglycan with chondroitin sulphate and dermatan sulphate side chains has been isolated and purified from a yolk sac tumour of the left ovary from a 23-year-old female. A monoclonal antibody, designated 2B1, was produced which reacted specifically with the intact molecule of the large proteoglycan and the chondroitinase ABC-treated core molecule. The localization of substances showing cross-reactivity to this antibody was studied in a variety of human tissues by means of indirect immunohistochemistry. The interstitial elements of nearly all tissues of a 5-month-old foetus were intensely reactive with the antibody, but in adult tissues structures that gave positive reactions were limited; only the perivascular and perimuscular fibrous elements were reactive, except for the aorta, which reacted extensively. In contrast, the interstitial elements of the carcinoma tissues tested were intensely reactive. Thus antibody 2B1 can be regarded as a useful tool for studies on the immunohistochemical localization of large proteoglycan in various human tissues.

Keywords

Human Tissue Chondroitin Sulphate Adult Tissue Carcinoma Tissue Dermatan Sulphate 

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Copyright information

© Chapman and Hall Ltd 1989

Authors and Affiliations

  • Mitsuko Sobue
    • 1
  • Nobuo Nakashima
    • 2
  • Toshiaki Fukatsu
    • 2
  • Tetsuro Nagasaka
    • 1
  • Shinji Fukata
    • 1
  • Noboru Ohiwa
    • 1
  • Yoshiharu Nara
    • 1
  • Takashi Ogura
    • 3
  • Takashi Katoh
    • 3
  • Jun Takeuchi
    • 1
    • 2
  1. 1.Department of Laboratory MedicineNagoya University School of MedicineJapan
  2. 2.Division of Pathology, Clinical LaboratoryNagoya University HospitalNagoyaJapan
  3. 3.Tokyo Research InstituteSeikagaku Kogyo Company LtdTokyoJapan

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