Abstract
Population genetic studies were carried out on 3 ethnic subpopulations living in Brussels (119 Belgians, 120 Turks and 137 Moroccans). DNA extraction was performed using the Chelex method. After DNA amplification the DNA fragments were separated electrophoretically in horizontal polyacrylamide gels. A total of 32 alleles (between 21 and 25 alleles in each subpopulation) including 8 “interalleles” could be differentiated. The allele frequencies were compared with population data from a German study and no significant differences could be observed.
This is a preview of subscription content, log in via an institution to check access.
References
Budowle B, Chakraborty R, Giusti AM, Eisenberg AJ, Allen RC (1991) Analysis of the variable number of tandem repeats locus D1S80 by the polymerase chain reaction followed by high resolution polyacrylamide gel electrophoresis. Am J Hum Genet 48:137–144
Jones DA (1972) Blood samples: probability of discrimination. J Forensic Sci Soc 12:355–359
Kasai K, Nakamura Y, White R (1990) Amplification of a variable number of tandem repeats (VNTR) locus (pMCT 118) by the polymerase chain reaction (PCR) and its application to forensic science. J Forensic Sci 35: 1196–1200
Kloosterman AD, Budowle B, Daselaar P (1993) PCR — amplification and detection of the human D1S80 VNTR locus. Amplification conditions, population genetics and application in forensic analysis. Int J Legal Med 105:257–264
Krüger J, Fuhrmann W, Lichte KH, Steffens C (1968) Zur Verwendung des Polymorphismus der sauren Erythrocytenphosphatase bei der Vaterschaftsbegutachtung. Dtsch Z Gerichtl Med 64:127–146
Rand S, Puers C, Skowasch K, Wiegand P, Budowle B, Brinkmann B (1992) Population genetics and forensic efficiency data of 4 AMPFLP's. Int J Legal Med 104:329–333
Skowasch K, Wiegand P, Brinkmann B (1992) pMCT 118 (D1S80): a new allelic ladder and an improved electrophoretic separation lead to the demonstration of 28 alleles. Int J Legal Med 105:165–168
Walsh PS, Metzger DA, Higuchi R (1991) Chelex® 100 as a medium for simple extraction of DNA for PCR — based typing from forensic material. Biotechniques 10:506–513
Wiegand P, Bajanowski T, Brinkmann B (1993) PCR typing of debris from fingernails. Int J Legal Med 106: 81–84
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sepulchre, M.A., Wiegand, P. & Brinkmann, B. D1S80 (pMCT118): analysis of 3 ethnic subpopulations living in Brussels. Int J Leg Med 108, 45–47 (1995). https://doi.org/10.1007/BF01845618
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01845618