Estimation of chronologic age using the aspartic acid racemization method. I. On human rib cartilage
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Determinations of chronologic age are of great importance in forensic science. At present the aspartic acid racemization method on teeth provides one of the best means in adult individuals. However, if teeth are not available, some other stable tissue has to be used. In this study, the applicability of cartilage from the ribs has been tested. Specimens of rib cartilage were obtained at autopsy from 24 individuals aged 0.2–96 years. An acid-soluble peptide (SP) fraction and an insoluble collagen-rich (IC) fraction were prepared, and the ratio of D/L aspartic acid was determined using the HPLC technique. The correlation coefficient between the D/L ratio and age was r = 0.91 in SP and r = 0.97 in IC. It thus seems as if cartilage from non-weight-bearing areas may be a useful source of tissue for the estimation of chronologic age with the aspartic acid racemization method when teeth are not available.
Key wordsAge determination Cartilage Aspartic acid High pressure liquid chromatography
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- 5.Ogino T, Ogino H (1988) Application to forensic odontology of aspartic acid racemization in unerupted and supernumerary teeth. J Dent Res 67: 1319–1322Google Scholar
- 6.Ohtani S, Kato S, Sugeno H, Sugimoto H, Marumo T, Yamazaki M, Yamamoto K (1988) A study on the use of the amino-acid racemization method to estimate the ages of unidentified cadavers from their teeth. Bull Kanagawa Dent Coll 16:11–21Google Scholar
- 19.Masters PM, Bada JL, Zigler JS Jr (1977) Aspartic acid racemization in the human lens during ageing and in cataract formation. Science 268:71–73Google Scholar
- 21.Ritz S, Turzynski A, Martz W, Schdtz HW (1992) In-vivoRazemisierung von Asparaginsdure in nicht-kollagenen Knochenpeptiden und Lebensaltersbestimmung an der Leiche. Zbl Rechtsmed 38:19Google Scholar
- 23.Maroudas A (1980) Metabolism of cartilaginous tissues: a quantitative approach. In: Maroudas A, Holborow EJ (eds) Studies in joint diseases. Pitman Medical, London, pp 59–66Google Scholar
- 24.Lippiello L, Munkin HJ (1976) Collagen turnover in osteoarthritis. Trans Orthop Res Soc 1:94–98Google Scholar
- 28.Pfeiffer H, Mömstad H, Teivens A (1995) Estimation of chronologic age using the aspartic acid racemization method. II. On human cortical bone. Int J Legal MedGoogle Scholar