Abstract
Antibodies to DR antigens were detected using serological and immunochemical tests in sera from rabbits and goats immunized with cultured human B-lymphoid cells mixed with an anti-T-cell xenoantiserum or with partially purified DR antigens. After absorption with human red blood cells, cultured melanoma cells, and/or T-lymphoid cells, DR xenoantisera become specifically cytotoxic to B lymphocytes. Three out of nine sera tested with a panel of T-depleted peripheral lymphocytes and chronic lymphocytic leukemia cells showed correlation with DR alloantisera submitted to the Seventh International Histocompatibility Workshop. Although the correlation coefficients were lower than those obtained with DR alloantisera, the results obtained suggest that DR xenoantisera may recognize allotypic specificities.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albrechtsen, D., Solheim, B.G., and Thorsby, E.: Antiserum inhibition of the mixed lymphocyte culture (MLC) interaction. Inhibitory effect of antibodies reactive with HLA-D-associated determinants.Cell. Immunol. 28:258–273, 1977
Allison, J.P., Belvedere, M., Reisfeld, R.A., Pellegrino, M.A., and Ferrone, S.: Serological and immunochemical characterization of HLA-A9 xenoantisera.J. Immunol. 121:579–585, 1978a
Allison, J.P., Walker, L.E., Russell, W.A., Pellegrino, M.A., Ferrone, S., Reisfeld, R.A., Frelinger, J.A., and Silver, J.: Murine Ia and human DR antigens: Homology of amino terminal sequences.Proc. Natl. Acad. Sci. U.S.A.75:3953–3956, 1978b
Cullen, S.E. and Schwartz, B.A.: An improved method for isolation of H-2 and Ia alloantigens with immunoprecipitation induced by protein A-bearing staphylococci.J. Immunol. 117:136–142, 1976
Einstein, A.B. jr., Mann, D.L., Gordon, H.G., Trapani, R.J., and Fahey, J.L.: Heterologous antisera against HLA antigens.Transplantation 12:299–304, 1971
Ferrone, S., Mickey, M.R., Terasaki, P.I., Reisfeld, R.A., and Pellegrino, M.A.: Humoral sensitization in parous women of cytotoxic antibodies to non-HLA antigens.Transplantation 22:61–68, 1976
Ferrone, S., Pellegrino, M.A., and Reisfeld, R.A.: Immunogenicity of human B cell antigens solubilized from cultured human lymphoid cells.J. Immunol. 118:1036–1041, 1977
Geier, S.S. and Cresswell, P.: Rabbit antisera to human B cell alloantigens: Effect on the mixed lymphocyte response.Cell. Immunol. 28:341–354, 1977
Hartzman, R.J., Segall, M., Bach, M.L., and Bach, F.H.: Histocompatibility matching. VI. Miniaturization of the mixed leukocyte cultured test: A preliminary report.Transplantation 11:268–273, 1971
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227:680–685, 1970
Nisonoff, A., Wissler, F.C., and Woernly, D.L., Properties of univalent fragments of rabbit antibody isolated by specific adsorption.Arch. Biochem. Biophys. 88:241–249, 1960
Pellegrino, M.A., Ferrone, S., and Theofilopoulos, A.N.: Isolation of human T and B lymphocytes by rosette formation with 2-aminoethylisothiouronium bromide (AET) treated sheep red blood cells and with monkey red blood cells.J. Immunol. Methods 11:273–279, 1976
Reisfeld, R.A., Sevier, E.D., Pellegrino, M.A., Ferrone, S., and Poulik, M.D.: Association of HLA antigens and β2-microglobulin at the cellular and molecular level.Immunogenetics 2:183–197, 1975
Richiardi, P., Curtoni, E.S., Piazza, A., and Mattiuz, P.L.: Identification of two determinants induced in "Antigen” HLA-A-10 (To 31 and To 40) and of a new family of cross-reacting specificities (HLA-1, 11, To 30, 31,40).Tissue Antigens 1:162–170, 1971
Solheim, B.G., Fuks, A., Smith, L., Strominger, J., and Thorsby, E.: Possible detection of HLA-DR alloantigenic specificities in man with unabsorbed rabbit antiserum.Scand. J. Immunol. 8:15–20, 1978
Springer, A.T., Kaufman, J.F., Siddoway, L.A., Mann, D.L., and Strominger, J.L.: Purification of HLA-linked B lymphocyte alloantigens in immunologically active form by preparative sodium dodecyl sulfate-gel electrophoresis and studies on their subunit association.J. Biol. Chem. 252:6201–6207, 1977a
Springer, T.A., Kaufman, J.F., Terhorst, C., and Strominger, J.L.: Purification and structural characterization of human HLA-linked B-cell antigens.Nature 268:213–218, 1977b
Ting, A., Mickey, M.R., and Terasaki, P.I.: B-lymphocyte alloantigens in Caucasians.J. Exp. Med. 143:981–986, 1976
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ferrone, S., Naeim, F., Indiveri, F. et al. Xenoantisera to human DR antigens: Serological and immunochemical characterization. Immunogenetics 7, 349–358 (1978). https://doi.org/10.1007/BF01844024
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01844024