Skip to main content
SpringerLink
Log in

Host-determined T cell fine specificity for self-H-2 in radiation bone-marrow chimeras of standard C57BL/6 (H-2b) mutantHz1 (H-2ba), and F1 mice

  • Original Investigations
  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

Reciprocal radiation bone-marrow chimeras were produced between the standard C57BL/6 (=B6) and the mutant B6.C-H-2 ba (=Hz1) strain. When infected with vaccinia virus, these chimeras, as well as an (Hz1 × B6)=→ Hz1 chimera, produced cytotoxic cells that killed vaccinia-infected H-2KkH-2Db target cells but failed to kill virus-infected H-2KbH-2Dd cells. Virus-infected (Hz1 × B6)F1 → B6 chimeras, however, killed both types of target. These experiments demonstrate strict T-cell specificity capable of differentiating between two molecules that apparently differ by a single amino acid substitution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bevan, M.J.: In a radiation chimera host H-2 antigens determine the immune responsiveness of donor cytotoxic cells.Nature 269:417 1977

    Article  CAS  PubMed  Google Scholar 

  • Blanden, R.V., Dunlop, M.B.C., Doherty, P.C., Kohn, H.I., and McKenzie, I.F.C.: Effects of fourH-2K mutations on virus-induced antigens recognized by cytotoxic T Cells.Immunogenetics 3:541–548, 1976

    Article  Google Scholar 

  • Brown, J.L. and Nathenson, S.G.: Structural differences between parent and mutant H-2K glycoproteins from two H-2K gene mutants: B6-C-H-2ba (Hz1) and B6-H-2bd (M505).J. Immunol. 118:98–104, 1976

    Google Scholar 

  • Doherty, P.C., Blanden, R.V., and Zinkernagel, R.M.: Specificity of virus-immune effector T cells for H-2K or H-2D compatible interactions: Implications for H-antigen diversity.Transplant. Rev. 29:89–124, 1967

    Google Scholar 

  • Forman, J. and Klein, J.: Analysis of H-2 mutants: Evidence for multiple CML target specificities controlled by the H-2Kb gene.Immunogenetics 1:469–481, 1975

    Article  Google Scholar 

  • Klein, J.:Biology of the Mouse Histocompatibility-2 Complex, Springer-Verlag, New York, 1975

    Google Scholar 

  • Klein, J.:H-2 mutations: Their genetics and effect on immune functions,Adv. Immunol. 26:55–146, 1978

    Article  CAS  PubMed  Google Scholar 

  • Klein, P.: Anomalous reactions of mouse alloantisera with culture tumor cells. I. Demonstration of widespread occurrence using reference typing sera.J. Immunol. 115:1254–1260, 1975

    CAS  PubMed  Google Scholar 

  • McKenzie, I. F. C., Pang, T., and Blanden, R.V.: The use of H-2 mutants as models for the study of T cell activation.Immunol. Rev. 35:81–130, 1977

    Google Scholar 

  • Melief, J.M., van der Meulen, M.Y., and Postma, P.: CML typing of serologically identicalH-2 mutants. Distinction of 19 specificities on the cells of four mouse strains carryingzl locus mutations and the strain of origin.Immunogenetics 5:43–56, 1977

    Article  Google Scholar 

  • Nabholz, M., Young, H., Meo, T., Miggiano, V., Rijnbeck, A., and Shreffler, D.C.: Genetic analysis of an H-2 mutant, B6.C-H-2ba, using cell-mediated lympholysis: T- and B-cell dictionaries for histo-compatibility determinants are different.Immunogenetics 1:457–468, 1975

    Article  Google Scholar 

  • Sprent, J., von Boehmer, H., and Nabholz, M.: Association of immunity and tolerance to host H-2 determinants in irradiated F1 hybrid mice reconstituted with bone marrow cells from one parental strain.J. Exp. Med. 142:321, 1975

    Article  CAS  PubMed  Google Scholar 

  • Zinkernagel, R.M.: H-2 compatibility requirement for virus-specific T cell-mediated cytolysis. The H-2K structure involved is coded by a single cistron defined by H-2Kb mutant mice.J. Exp. Med. 142:437–443, 1976

    Article  Google Scholar 

  • Zinkernagel, R.M., Callahan, G.N., Klein, J., Dennert, G.: Cytotoxic T cells learn specificity for self H-2 during differentiation in the thymus.Nature 271:25–253, 1978a

    Article  Google Scholar 

  • Zinkernagel, R.M., Callahan, G.M., Althage, A., Cooper, S., Klein, P.A., and Klein, J.: On the thymus in differentiation of “H-2 self-recognition” by T cells: Evidence for dual recognition?J. Exp. Med. 147:882–896, 1978b

    Article  CAS  PubMed  Google Scholar 

  • Zinkernagel, R.M., Callahan, G.M., Althage, A., Cooper, S., Streilein, J.W., and Klein, J.: The lymphoreticular system in triggering virus-plus-self-specific cytotoxic T cells: Evidence for T help.J. Exp. Med. 147:897–911, 1978c

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cellular and Developmental Immunology, Scripps Clinic and Research Foundation, 92037, La Jolla, California

    Rolf M. Zinkernagel

  2. Department of Pathology, College of Medicine, University of Florida, 32610, Gainesville, Florida

    Paul A. Klein

  3. Department of Microbiology, Southwestern Medical School, University of Texas, 75235, Dallas, Texas

    Jan Klein

Authors
  1. Rolf M. Zinkernagel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paul A. Klein
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jan Klein
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zinkernagel, R.M., Klein, P.A. & Klein, J. Host-determined T cell fine specificity for self-H-2 in radiation bone-marrow chimeras of standard C57BL/6 (H-2b) mutantHz1 (H-2ba), and F1 mice. Immunogenetics 7, 73–77 (1978). https://doi.org/10.1007/BF01843990

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01843990

Keywords

Search

Navigation