Advertisement

Immunogenetics

, Volume 7, Issue 1, pp 13–16 | Cite as

On the selective elimination of Y-bearing sperm

  • Susumu Ohno
  • Stephen S. Wachtel
Original Investigations

Abstract

The potential use of antibodies that selectively recognize either X-bearing or Y-bearing sperm is self-evident. Thus our attention was directed to the fact that under optimal conditions, H-Y antibody lyses 50% of mouse spermatozoa. Accordingly, we asked whether expression of H-Y antigen is haploid in spermatozoa from XY male mice heterozygous for the autosomal dominantSxr gene, for if H-Y expression were haploid, H-Y antibody would be expected to kill 75% of spermatozoa derived from these XY,Sxr/- males. However, maximal lysis remained at the 50% level, which indicates that haploid expression of H-Y antigen and the potential immunoselection of Y-(or X-) bearing spermatozoa are unlikely.

Keywords

Blood Group Antigen Intercellular Bridge Transmission Ratio Distortion Mouse Epidermal Cell Wood Lemming 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arnaiz-Villena, A. and Festenstein, H.: HLA genotyping by using spermatozoa: evidence for haploid gene expression.Lancet 2:707–709, 1976CrossRefPubMedGoogle Scholar
  2. Bailey, D.W.: Histoincompatibility associated with the X-chromosome in mice.Transplantation 1:70–74, 1963CrossRefPubMedGoogle Scholar
  3. Bennett, D. and Boyse, E.A.: Sex ratio in progeny of mice inseminated with sperm treated with H-Y antiserum.Nature 246:308–309, 1973CrossRefPubMedGoogle Scholar
  4. Bennett, D., Boyse, E.A., Mathieson, B. J., Scheid, M., Yanigisawa, K., Wachtel, S., and Cattanach, B.M.: Serological evidence for H-Y antigen inSxr, XX sex-reversed phenotypic males.Nature 265:255–257, 1977CrossRefPubMedGoogle Scholar
  5. Buck, D.W. and Bodmer, W.F.: Serological identification of an X-linked human cell surface antigen, SA-X.Birth Defects 12:376–381, 1976PubMedGoogle Scholar
  6. Cattanach, B.M., Pollard, C.E., and Hawkes, S.G.: Sex-reversed mice: XX and XO males.Cytogenetics 10:318–337, 1971CrossRefPubMedGoogle Scholar
  7. Fawcett, D.W., Ito, S., and Slautterback, D.: The occurrence of intercellular bridges in groups of cells exhibiting synchronous differentiation.J. Biophys. Biochem. Cytol. 5:453–460, 1959CrossRefPubMedGoogle Scholar
  8. Fellous, M. and Dausset, J.: Probable haploid expression of HL-A antigens on human spermatozoa.Nature 225:191–193, 1970CrossRefPubMedGoogle Scholar
  9. Fredga, K., Gropp, A., Winking, H., and Frank, F.: Fertile XX- and XY-type females in the wood lemmingMyopus schisticolor.Nature 261:225–227, 1976CrossRefPubMedGoogle Scholar
  10. Goldberg, E.H., Boyse, E.A., Bennett, D., Scheid, M., and Carswell, E.A.: Serological demonstration of H-Y (male) antigen on mouse sperm.Nature 232:478–480, 1971CrossRefPubMedGoogle Scholar
  11. Nagai, Y. and Ohno, S.: Testis-determining H-Y antigen in XO males of the mole-vole (Ellobius lutescens).Cell 10:729–732, 1977CrossRefPubMedGoogle Scholar
  12. Ohno, S.:Sex Chromosomes and Sex-Linked Genes. Springer-Verlag, Berlin, 1967iGoogle Scholar
  13. Popivanov, R. and Vulchanov, V.H.: Segregation of man's AB-group spermatozoa in A- and B- spermatozoa through agglutination with immune anti-A rabbit serum.Z. Immunitaetsforsch. 124:206–210, 1962PubMedGoogle Scholar
  14. Scheid, M., Boyse, E.A., Carswell, E.A., and Old, L.J.: Serologically demonstrable alloantigens of mouse epidermal cells.J. Exp. Med. 135:938–955, 1972CrossRefPubMedGoogle Scholar
  15. Shahani, S. and Southam, A.L.: Immunofluorescent study of the ABO blood group antigens in human spermatozoa.Am. J. Obstet. Gynecol. 84:660–666, 1962PubMedGoogle Scholar
  16. Shalev, A., Berczi, I., and Hamerton, J.: Detection and crossreaction of H-Y antigen by hemagglutination.J. Immunogenet., in press, 1978Google Scholar
  17. Wachtel, S.S., Koo, G.C., Ohno, S., Gropp, A., Dev, V.G., Tantravahi, R., Miller, D.A., and Miller, O.J.: H-Y antigen and the origin of XY female wood lemmings (Myopus schisticolor).Nature 264:638–639, 1976CrossRefPubMedGoogle Scholar
  18. Wachtel, S.S., Koo, G.C., Zuckerman, E., Hammerling, U., Scheid, M., and Boyse, E.A.: Serological cross-reactivity between H-Y (male) antigens of mouse and man.Proc. Natl. Acad. Sci. U.S.A. 71:1215–1218, 1974CrossRefPubMedGoogle Scholar
  19. Wachtel, S.S., Ohno, S., Koo, G.C., and Boyse, E.A.: Possible role for H-Y antigen in the primary determination of sex.Nature 257:235–236, 1975CrossRefPubMedGoogle Scholar
  20. Yanagisawa, K., Pollard, D.R., Bennett, D., Dunn, L.C., and Boyse, E.A.: Transmission ratio distortion at the T-locus: Serological identification of two sperm populations in t-heterozygotes.Immunogenetics 1:91–96, 1974;CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1978

Authors and Affiliations

  • Susumu Ohno
    • 1
  • Stephen S. Wachtel
    • 2
  1. 1.Department of BiologyCity of Hope National Medical CenterDuarte
  2. 2.Memorial Sloan-Kettering Cancer CenterNew York

Personalised recommendations