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Journal of Muscle Research & Cell Motility

, Volume 11, Issue 3, pp 191–202 | Cite as

Striated muscle tropomyosin-enriched microfilaments of developing muscles of chicken embryos

  • Seu-Mei Wang
  • Seu-Hwa Wang
  • Jenny Li-Chun Lin
  • Jim Jung-Ching Lin
Papers

Summary

The striated muscle tropomyosin-enriched microfilaments were isolated from developing musclesin ovo by the previously described method with a monoclonal antibody against striated muscle isoforms of tropomyosin (Lin & Lin, 1986). Two-dimensional gel analysis of the isolated microfilaments from developing heart, thigh and breast muscles revealed the coexistence of non-muscle isoforms of tropomyosin and actin throughout all stages of embryogenesis. A small but significant amount of skeletal muscle isoforms (α, β) of tropomyosins and their phosphorylated forms was detected in the microfilaments isolated from hearts of 6–15-day-old embryos. These skeletal isoforms of tropomyosins disappeared after this stage of embryogenesis. In addition, we also detected both embryonic and adult isoforms of troponin T in early developing hearts. In developing thigh and breast muscles, the presence of non-muscle tropomyosin isoforms 2, 3a and 3b in the isolated microfilaments was apparent. The contents of tropomyosin isoform 2 were decreased with development and this non-muscle isoform completely disappeared at the 15th day of embryogenesis. On the other hand, the non-muscle tropomyosin isoforms 3a and 3b were present throughout all stages of development. Double-label immunofluorescence microscopy with monoclonal CH1 (anti-striated muscle isoforms of tropomyosin) and CGβ6 (anti-non-muscle isoforms of tropomyosin) on the isolated, glycerinated skeletal and cardiac muscle cells of 10-day-old or 13-day-old embryos confirmed the colocalization of muscle and non-muscle isoforms of tropomyosins within the same cells. These results suggest that different isoforms of actin and tropomyosin can assemble into a class of microfilaments (i.e. striated muscle tropomyosin-enriched microfilaments)in ovo, which may transform into the thin filaments of mature muscle cells.

Keywords

Cardiac Muscle Immunofluorescence Microscopy Chicken Embryo Thin Filament Phosphorylated Form 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall Ltd 1990

Authors and Affiliations

  • Seu-Mei Wang
    • 1
  • Seu-Hwa Wang
    • 1
  • Jenny Li-Chun Lin
    • 2
  • Jim Jung-Ching Lin
    • 2
  1. 1.Department of Anatomy, College of MedicineNational Taiwan UniversityTaipeiTaiwan
  2. 2.Department of BiologyUniversity of IowaIowa CityUSA

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