Veterinary Research Communications

, Volume 19, Issue 5, pp 425–432 | Cite as

Some pharmacokinetic parameters of doxycycline in East African goats after intramuscular administration of a long-acting formulation

  • I. M. Ole-Mapenay
  • E. S. Mitema
Pharmacology

Abstract

A compartmental and non-compartmental study was carried out on five adult goats following intramuscular administration of doxycycline at 20 mg/kg bodyweight. The concentration of the drug in serum was determined by a microbiological assay employingBacillus cereus varmycoides (ATCC 11778) as the test organism. The mean serum concentration (Cmax) and the time of maximum concentration (Tmax) were 1.87 µg/ml and 0.85 h, respectively. Using compartmental analysis, the plasma concentration-time curve of doxycycline best fitted a three-compartment open model with first-order absorption. A three-phase disposition of doxycycline was found, the terminal elimination half-life being approximately 40 h.

The statistical moment theory was mainly used for non-compartmental analysis. The value obtained for the mean residence time (MRT) was 16.41 h. The mean values for the volume of distribution at steady state (Vdss), determined by compartmental and non-compartmental analyses, were 8.73 and 13.19 L/kg, respectively. There were no statistically significant differences when the major pharmacokinetic parameters were compared.

It was concluded that the pharmacokinetic behaviour of doxycycline in goats after intramuscular administration is characterized by a three-compartment model with a slow terminal elimination phase. Based on current knowledge, this could be due to enterohepatic recycling and/or flip-flop kinetics. The study indicated that a single intramuscular administration of 20 mg/kg of doxycycline may only provide therapeutic concentrations for up to 24 h owing to slow absorption at the injection site.

Keywords

doxycycline goat intramuscular pharmacokinetics 

Abbreviations

ATCC

American Type Culture Collection

AVC

total area under the plasma concentration-time curve

AUMC

area under the curve of the product from time zero to infinity

C1

total body clearance

i.m.

intramuscular

i.v.

intravenous

MRT

mean residence time

MIC

minimum inhibitory concentration

PVP

polyvinyl pyrolidone

V

volume of distribution

Vdss

volume of distribution at steady state

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Copyright information

© Kluwer Academic Publishers bv 1995

Authors and Affiliations

  • I. M. Ole-Mapenay
    • 1
  • E. S. Mitema
    • 1
  1. 1.Department of Public Health, Pharmacology and Toxicology, Faculty of Veterinary MedicineUniversity of NairobiNairobiKenya

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