Veterinary Research Communications

, Volume 19, Issue 4, pp 265–271 | Cite as

Genotyping of isolates ofTaylorella equigenitalis from thoroughbred brood mares in Japan

  • T. Miyazawa
  • M. Matsuda
  • Y. Isayama
  • T. Samata
  • Y. Ishida
  • S. Ogawa
  • K. Takei
  • M. Honda
  • M. Kamada
Bacteriology

Abstract

Profiles of the genomic DNA of 104 strains ofT. equigenitalis isolated from brood mares with contagious equine metritis in Hokkaido during the breeding seasons from 1980 to 1993, as well as those of five strains (SS28, EQ56, EQ59, EQ70 and HH139) previously isolated in Japan were examined after restriction digestion and crossed-field gel electrophoresis. These profiles were essentially identical to each other and the various isolates and strains appeared to have a common genotype, designated ‘genotype J’, with respect to two restriction enzymes,ApaI andNotI. These results suggest a common source for all these isolates obtained over the course of more than 10 years in Japan.

Keywords

contagious equine metritis DNA genome pulsed-field gel electrophoresis strain identification Taylorella equigenitalis 

Abbreviations

CEM

contagious equine metritis

CFGE

crossed-field gel electrophoresis

PFGE

pulsed-field gel electrophoresis

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References

  1. Bleumink-Pluym, N., Ter Laak, E.A. and Van Der Zeijst, B.A.M., 1990. Epidemiologic study ofTaylorella equigenitalis strains by field inversion gel electrophoresis of genomic restriction endonuclease fragments.Journal of Clinical Microbiology,28, 2012–2016PubMedGoogle Scholar
  2. Kamanda, M., Akiyama, Y., Oda, T. and Fukuzawa, Y., 1981. Contagious equine metritis: isolation ofHaemophilus equigenitalis from horses with endometritis in Japan.Japanese Journal of Veterinary Science,43, 565–568PubMedGoogle Scholar
  3. Kamada, M., Oda, T., Fukuzawa, Y., Ohishi, H., Wada, R., Fukunaga, Y. and Kumanomido, T., 1983. Contagious equine metritis: a survey on complement fixing antibody againstHaemophilus equigenitalis in light horses in Japan.Bulletin of the Equine Research Institute,20, 119–125Google Scholar
  4. Kikuchi, N., Tsunoda, N., Kawakami, Y., Murase, N. and Kawata, K., 1982. An outbreak of contagious equine metritis in Japan: isolation ofHaemophilus equigenitalis from thoroughbred mares with genital infection in Hokkaido.Japanese Journal of Veterinary Science,44, 107–114PubMedGoogle Scholar
  5. Matsuda, M., Asami, Y., Miyazawa, T., Samata, T., Isayama, Y., Honda, M. and Ide, Y., 1994. Analysis of chromosome-sized DNA and genome typing of isolated strains ofTaylorella equigenitalis.Veterinary Research Communications,18, 93–98PubMedGoogle Scholar
  6. Miyazawa, T., Matsuda, M., Kumano, M., Isayama, Y., Honda, M. and Ogawa, S., 1994. Estimation of the genome size of five strains ofTaylorella equigenitalis in Japan.Veterinary Research Communications,18, 165–168PubMedGoogle Scholar
  7. Sugimoto, C., Isayama, Y., Kashiwazaki, M., Fujikura, T. and Mitani, K., 1980. Detection ofHaemophilus equigenitalis, the causal agent of contagious equine metritis, in Japan.National Institute of Animal Health Quarterly (Japan),20, 118–119PubMedGoogle Scholar
  8. Sugimoto, C., Isayama, Y., Sakazaki, R. and Kuramochi, S., 1983. Transfer ofHaemophilus equigenitalis Tayloret al. 1978 to the genusTaylorella gen. nov. asTaylorella equigenitalis comb. nov..Current Microbiology,9, 155–162Google Scholar
  9. Taylor, C.E.D., Rosenthal, R.O., Brown, D.F.J., Lapage, S.P., Hill, L.R. and Legros, R.M., 1978. The causative organism of contagious equine metritis 1977: proposal for a new species to be known asHaemophilus equigenitalis.Equine Veterinary Journal,10, 136–144PubMedGoogle Scholar
  10. Ter Laak, E.A., Fennema, G. and Jaartsveld, F.H.J., 1989. Contagious equine metritis in the Netherlands.Tijdschrift voor Diergeneeskunde,114, 189–201PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers bv 1995

Authors and Affiliations

  • T. Miyazawa
    • 1
  • M. Matsuda
    • 1
  • Y. Isayama
    • 2
  • T. Samata
    • 1
  • Y. Ishida
    • 1
  • S. Ogawa
    • 1
  • K. Takei
    • 1
  • M. Honda
    • 3
  • M. Kamada
    • 4
  1. 1.Laboratory of Molecular Biology, Department of Biosciences College of Environmental HealthAzabu UniversitySagamihara
  2. 2.Department of Immunology College of Environmental HealthAzabu UniversitySagamihara
  3. 3.Department of Hygiene, College of Environmental HealthAzabu UniversitySagamihara
  4. 4.Epizotic Research Station, Equine Research InstituteJapan Racing AssociationTochigiJapan

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