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Evaluation of an automated spectrophotometric assay for the determination of total sialic acid in canine serum

  • Biochemistry
  • Published:
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Abstract

This study validates an automated enzymatic assay using the Cobas Fara (Roche) centrifugal analyser, which offers a reliable measurement of the total sialic acid concentration in canine serum as assessed by evaluating the precision and accuracy. Data are presented on the biological variation in the total serum sialic acid concentration. Measurements of total serum sialic acid concentration appear to be useful in distinguishing dogs with neoplastic disorders from clinically healthy dogs.

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Abbreviations

AcNeu:

N-acetylneuraminic acid

CV%:

coefficient of variation

LASA:

lipid-associated sialic acid

S 2 :

component of variance

References

  • Allen, S.W., Prasse, K.W. and Mahaffey, E.A., 1986. Cytologic differentiation of benign from malignant canine mammary tumors.Veterinary Pathology,23, 649–655

    Google Scholar 

  • Armitage, P. and Berry, G., 1987. Statistical interference. In: P. Armitage and G. Berry (eds.),Statistical Methods in Medical Research, 2nd edn., (Blackwell Scientific Publications, Oxford), 93–140

    Google Scholar 

  • Crook, M., 1993. The determination of plasma or serum sialic acid.Clinical Biochemistry,26, 31–38

    Google Scholar 

  • Fayolle, P., Broulet, V., Braun, J.P., Rico, A.G., Magnol, J.P. and Thouvenot, J.P., 1987. Routine hematology and clinical chemistry in female dogs with benign and malignant mammary tumors.Veterinary Clinical Pathology,16, 73–75

    Google Scholar 

  • Fraser, C.G., 1983. Desirable performance standards for clinical chemistry tests.Advances in Clinical Chemistry,23, 299–339

    Google Scholar 

  • Fraser, C.G. and Harris, E.G., 1989. Generation and application of data on biological variation in clinical chemistry.CRC Critical Review of Clinical Laboratory Science,27, 409–430

    Google Scholar 

  • Freund, R.J. and Littell, R.C., 1991. A three-factor response surface experiment. In: J. Lopez, P.R. Sheldon and K. Whatley (eds.),SAS System for Regression, 2nd edn, (SAS Institute Inc., Cary), 143–150

    Google Scholar 

  • Horiuchi, T. and Kurokawa, T., 1989. New enzymatic endpoint assay of serum sialic acid.Clinica Chimica Acta,182, 117–122

    Google Scholar 

  • Jensen, A.L. and Aaes, H., 1992. Reference interval and critical difference for canine serum fructosamine concentration.Veterinary Research Communications,16, 317–325

    Google Scholar 

  • Kloppel, T.M., Franz, C.P., Morré, D.J. and Richardson, R.C., 1978. Serum sialic acid levels increased in tumor-bearing dogs.American Journal of Veterinary Research,39, 1377–1380

    Google Scholar 

  • Ménard, M., Fontaine, M. and Morin, M., 1986. Fine needle aspiration biopsy of malignant tumors in dogs and cats: A report of 102 cases.Canadian Veterinary Journal,27, 504–510

    Google Scholar 

  • Plucinsky, M.C., Riley, W.M., Prorok, J.J. and Alhadeff, J.A., 1986. Total and lipid-associated serum sialic acid levels in cancer patients with different primary sites and differing degrees of metastatic involvement.Cancer,58, 2680–2685

    Google Scholar 

  • Poli, A., Arispici, M., Camillo, F. and Corazza, M., 1986. Increase of serum lipid-associated sialic acid concentration in dogs with neoplasms.American Journal of Veterinary Research,47, 607–609

    Google Scholar 

  • Shamberger, R.J., 1986. Evaluation of water soluble and lipid soluble sialic acid levels as tumor markers.Anticancer Research,6, 717–720

    Google Scholar 

  • Stefenelli, N., Klotz, H., Engel, A. and Bauer, P., 1985. Serum sialic acid in malignant tumors, bacterial infections, and chronic liver diseases.Journal of Cancer Research and Clinical Oncology,109, 55–59

    Google Scholar 

  • Sugahara, K., Sugimoto, K., Nomura, O. and Usui, T., 1980. Enzymatic assay of serum sialic acid.Clinica Chimica Acta,108, 493–498

    Google Scholar 

  • Végh, Zs., Kremmer, T., Boldizsar, M., Gesztesi, K.A. and Szajani, B., 1991. A re-evaluation of the lipid-bound sialic acid determination.Clinical Chimica Acta,203, 259–268

    Google Scholar 

  • Voigtmann, R., Pokorny, J. and Meinshausen, A., 1989. Evaluation and limitations of the lipid-associated sialic acid test for the detection of cancer.Cancer,64, 2279–2283

    Google Scholar 

  • Warren, L., Fuhrer, J.P. and Buck, C.A., 1972. Surface glycoproteins of normal and transformed cells: A difference determined by sialic acid and a growth-dependent sialyl transferase.Proceedings of the National Academy of the USA,7, 1838–1842

    Google Scholar 

  • Waters, P.J., Lewry, E. and Pennock, C.A., 1992. Measurement of sialic acid in serum and urine: clinical applications and limitations.Annals of Clinical Biochemistry,29, 625–637

    Google Scholar 

  • Weisberg, S., 1985.Applied Linear Regression, 2nd edn, (Wiley, New York)

    Google Scholar 

  • Yogeeswaran, G., 1983. Cell surface glycolipids and glycoproteins in malignant transformation.Advances in Cancer Research,38, 289–350

    Google Scholar 

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Authors and Affiliations

  1. Central Laboratory, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870, Frederiksberg C, Denmark

    A. V. Thougaard

  2. Small Animal Hospital, Department of Clinical Studies, The Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870, Frederiksberg C, Denmark

    A. L. Jensen & A. Wenck

Authors
  1. A. V. Thougaard
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  2. A. L. Jensen
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  3. A. Wenck
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Thougaard, A.V., Jensen, A.L. & Wenck, A. Evaluation of an automated spectrophotometric assay for the determination of total sialic acid in canine serum. Vet Res Commun 18, 337–345 (1994). https://doi.org/10.1007/BF01839284

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  • DOI: https://doi.org/10.1007/BF01839284

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