Changes in oestrogen, progesterone and epidermal growth factor receptor concentrations and affinities during the oestrous cycle in the normal mammary gland and uterus of dogs
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Changes in the concentrations and affinities of receptors for oestrogen (ER), progesterone (PR) and epidermal growth factor (EGF-R) were studied in mammary glands of healthy bitches with regard to age, the location in the mammary chain and the stage of the oestrous cycle. Uterus was used as the reference tissue for the evaluation of steroid receptors. Mammary and uterine samples from 7 healthy bitches were taken at five stages of the oestrous cycle in such a way that all the locations in the mammary chain were represented at each stage of the cycle (10 samples/dog). ER, PR and EGF-R were detected by biochemical assays using increasing concentrations of tritiated (steroids) or iodinated (EGF) ligands. A significant direct correlation was found between the ER and PR concentrations for mammary and uterine samples. No significant correlation was found between the steroid receptors and EGF-R concentrations. Mammary ER concentrations were significantly higher in bitches of 5 years of age or older than in younger ones; in posterior glands (4th and 5th pairs) than in anterior glands; and in the mid-luteal phase. Mammary PR did not vary significantly with age or location but was significantly lower in the early luteal phase than in other phases. A similar decrease in PR concentrations was observed in the uterus during the early luteal phase and uterine ER and PR concentrations were very low in the mid-luteal phase. Mammary EGF-R were not significantly higher in the early or mid-luteal phase than in pro-oestrus or anoestrus.
The differences observed between the uterine and mammary steroid receptor concentrations during the oestrous cycle could be due to different mechanisms for regulating steroid receptor expression in the two tissues. Mammary EGF-R concentrations may be linked, as in other species, to cellular proliferation and/or to the serum progesterone concentrations.
Keywordsage bitch dog epidermal growth factor mammary gland oestrogen oestrous cycle progesterone receptor uterus
bovine serum albumin
epidermal growth factor
receptor for epidermal growth factor
probability of error
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- Brodey, R.S., Goldschmidt, M.H. and Roszel, J.R., 1983. Canine mammary gland neoplasms.Journal of the American Animal Hospital Association,19, 61–90Google Scholar
- Concannon, P.W., 1991. Reproduction in the dog and cat. In: P.T. Cupps (ed.),Reproduction in Domestic Animals, (Academic Press, London), 517–554Google Scholar
- Dagnelie, P., 1973, 1975.Théorie et méthodes statistiques, applications agronomiques, (Presses Agronomiques, Gembloux)Google Scholar
- EORTC, Breast Cancer Cooperative Group, 1980. Revision of the standards for the assessment of hormone receptors in human breast cancer.European Journal of Cancer,16, 1513–1516Google Scholar
- Ewing, T.M., Murphy, L.J., Ng, M.L., Pang, G.Y.N., Lee, C.S.L., Atts, C.K.W. and Sutherland, R.L., 1989. Regulation of epidermal growth factor receptor by progestins and glucocorticoids in human breast cancer cell lines.International Journal of Cancer,44, 744–752Google Scholar
- Haslam, S.Z., 1987. Role of sex steroid hormones in normal mammary gland function. In: M.C. Neville and C.W. Daniel (eds),The Mammary Gland. Development, Regulation and Function, (Plenum Press, New York and London), 499–533Google Scholar
- Kienhuis, C.B.M., 1992.Measurement of the epidermal growth factor receptor, (PhD thesis, University of Nijmegen)Google Scholar
- Koenders, P.G., 1992. Epidermal growth factor receptor and its ligand(s): associations with prognosis of patients with breast cancer, (PhD thesis, University of Nijmegen)Google Scholar
- Milgrom, E., Luu Thi, M. and Baulieu, E.E., 1973. Control mechanisms of steroid hormone receptors in the reproductive tract.Acta Endocrinologica, (supplement 180), 350–397Google Scholar
- Rutteman, G.R. and Misdorp, W., 1986. Steroid receptor determinations in malignant mammary tumors and in nonaffected mammary glands in the dog.Annals of the New York Academy of Sciences,464, 438–440Google Scholar
- Rutteman, G.R., Foekens, J.A., Blankenstein, M.A., Vos, J.H. and Misdorp, W., 1990. EGF receptors in nonaffected and tumorous dog mammary tissue.European Journal of Cancer,26, 182 (abstract)Google Scholar
- Scatchard, G., 1949. The attraction of proteins for small molecules and ions.Annals of the New York Academy of Sciences,51, 660–672Google Scholar
- Valverius, E., Bates, S., Stampfer, M., Clark, R., McCormick, F., Salomon, D., Lippman, M. and Dickson, R., 1989. Transforming growth factor alpha production and epidermal growth factor receptor expression in normal and oncogene transformed human mammary epithelial cells.Molecular Endocrinology,3, 203–214PubMedGoogle Scholar
- Wouters-Ballman, P., Donnay, I., Devleeschouwer, N. and Verstegen, J., 1995. Iodination of mouse EGF with Chloramine T at 4°C: characterization of the iodinate peptide and comparison with other labelling methods.Journal of Receptor Research,15, in pressGoogle Scholar