Abstract
Ten parasite-free lambs were drenched with oxfendazole on days 0 and 28 and, one day after each drench, were injected with human erythrocytes and ovalbumin. Ten other antigen-injected lambs were not drenched (controls). Lymphocytes collected 3 days after each antigen injection and cultured in RPMI 1640 plus 5% fetal calf serum (FCS) and lymphocytes collected 3 days after the first and 3 and 7 days after the second antigen injection and cultured in 50% autologous serum had decreased blastogenic activity compared with control lymphocytes. After the second drench, decreased blastogenesis was seen with lymphocytes collected on days 3 and 7 and cultured in 5% FCS and concanavalin A (Con A) and on day 3 when cultured in 5% FCS and phytohaemagglutinin (PHA). Decreased blastogenesis was also seen with lymphocytes collected 7 and 29 days after the second injection of antigen and cultured in 50% autologous serum plus Con A and on days 3, 7 and 29 when cultured in 50% autologous serum and PHA.
Significantly depressed antibody responses to both antigens were seen after the second drench. The serum complement level was depressed 3 days after the second injection of antigen. Serum nitric oxide levels were significantly depressed 3 and 21 days after the first and 7 and 21 days after the second injection of antigen. There were no differences in levels of growth-promoting hormones but the drenched lambs gained significantly more weight than the controls.
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Abbreviations
- C′:
-
complement
- Con A:
-
concanavalin A
- cpm:
-
counts per minute
- EIA:
-
enzyme immunoassay
- FCS:
-
fetal calf serum
- IGF:
-
insulin-like growth factor
- oIGF-1:
-
ovine insulin-like growth factor-1
- PBS:
-
phosphate-buffered saline
- PHA:
-
phytohaemagglutinin
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Stankiewicz, M., Cabaj, W., Jonas, W.E. et al. Oxfendazole treatment of non-parasitized lambs and its effect on the immune system. Vet Res Commun 18, 7–18 (1994). https://doi.org/10.1007/BF01839256
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DOI: https://doi.org/10.1007/BF01839256