Abstract
A new diagnostic procedure was developed to detect the flagellar antigen ofCampylobacter jejuni andCampylobacter coli in canine faecal specimens and was tested on faecal samples from random-source dogs obtained from the local dog pound. Extraction of acid-soluble proteins was performed on faecal specimens and the extracted material was evaluated using species-specific monoclonal antibodies in an enzyme-linked immunosorbent assay. The assay detected allC. jejuni orC. coli infected specimens compared with direct selective faecal culture. One of 18 faecal specimens culture-negative forC. jejuni was identified as positive by the assay, i.e. a false positive rate of 1 of 18 (5.6%) and a corresponding specificity of 94.4%. These results suggest that the screening procedure developed to detect flagellar antigens ofC. jejuni andC. coli in canine faecal samples should be further investigated as a diagnostic alternative to culture.
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Abbreviations
- ELISA:
-
enzyme-linked immunosorbent assay
- PBS:
-
phosphate-buffered saline
- OD:
-
optical density
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Monfort, J.D., Bech-Nielsen, S. & Stills, H.F. Detection of flagellar antigen ofCampylobacter jejuni andCampylobacter coli in canine faeces with an enzyme-linked immunosorbent assay (ELISA) — New prospects for diagnosis. Vet Res Commun 18, 85–92 (1994). https://doi.org/10.1007/BF01839224
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DOI: https://doi.org/10.1007/BF01839224