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Detection of anticoagulant rodenticides (4-hydroxycoumarins) by thin-layer chromatography and reversed-phase high-performance liquid chromatography with fluorescence detection

  • Forensic Chemistry
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Abstract

The detection of 4-hydroxycoumarin rodenticides in poisoned domestic animals requires a highly sensitive method as tissue and serum levels of anticoagulants may be very low owing to rapid elimination, metabolism or post-mortem degradation. Thin-layer chromatography (TLC) and reversed-phase high-performance liquid chromatography (RP-HPLC) with fluorescence detection were used to identify the anticoagulants in spiked tissues and in suspicious samples. The analysis of ten suspicious samples highlighted the limitations of both methods. Only the three samples of baits were found positive by TLC whereas one of the five anticoagulants was detected in eight samples by RP-HPLC with fluorescence detection. Therefore, RP-HPLC with fluorescence detection proved to be the more sensitive method for detecting low levels of 4-hydroxycoumarins in blood serum, liver and ingesta, whereas TLC is usually sufficient for analysing baits.

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Abbreviations

RP-HPLC:

reversed-phase high-performance liquid chromatography

TLC:

thin-layer chromatography

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Author notes

  1. I. Rengel

    Present address: GSF, Institute of Toxicology, Ingolstädter Landstrasse 1, D-85758, Oberschleissheim, Germany

  2. A. Friedrich

    Present address: Klinge Pharma GmbH, Weihenstephaner Strasse 28, D-81673, Munich, Germany

Authors and Affiliations

  1. Institute of Pharmacology, Toxicology and Pharmacy, University of Munich, Germany

    I. Rengel & A. Friedrich

Authors
  1. I. Rengel
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  2. A. Friedrich
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Rengel, I., Friedrich, A. Detection of anticoagulant rodenticides (4-hydroxycoumarins) by thin-layer chromatography and reversed-phase high-performance liquid chromatography with fluorescence detection. Vet Res Commun 17, 421–427 (1993). https://doi.org/10.1007/BF01839210

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  • DOI: https://doi.org/10.1007/BF01839210

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