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Antimicrobial effect of combinations of EDTA-Tris and amikacin or neomycin on the microorganisms associated with otitis externa in dogs

  • Bacteriology
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Abstract

Combinations of EDTA-Tris and two aminoglycoside antibiotics (amikacin and neomycin) were tested for synergistic activities against the microorganisms associated with otitis externa in dogs and for the solutions' stability over time. Synergistic activity was observed when EDTA-Tris plus amikacin and EDTA-Tris plus neomycin were tested againstStaphylococcus intermedius, Proteus mirabilis, Pseudomonas aeruginosa, andEscherichia coli, but not againstCandida albicans. Stability studies over a 3-month period indicated that the test solutions were stable at room temperature and that their antimicrobial activity was maintained.

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Abbreviations

CFU:

colony-forming units

EDTA:

(ethylenedinitrilo)tetraacetic acid, disodium salt

FIC:

fractional inhibitory concentration

MBC:

minimal bactericidal concentration

MIC:

minimal inhibitory concentration

Tris:

tris(hydroxymethyl)aminomethane

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Authors and Affiliations

  1. Department of Medical Microbilogy, College of Veterinary Medicine, University of Georgia, 30602, Athens, Georgia, USA

    T. A. Sparks, R. E. Wooley & P. S. Gibbs

  2. Veterinary Teaching Hospital, College of Veterinary Medicine, University of Georgia, 30602, Athens, Georgia, USA

    D. T. Kemp

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  1. T. A. Sparks
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  2. D. T. Kemp
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  3. R. E. Wooley
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  4. P. S. Gibbs
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Sparks, T.A., Kemp, D.T., Wooley, R.E. et al. Antimicrobial effect of combinations of EDTA-Tris and amikacin or neomycin on the microorganisms associated with otitis externa in dogs. Vet Res Commun 18, 241–249 (1994). https://doi.org/10.1007/BF01839190

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