Breast Cancer Research and Treatment

, Volume 21, Issue 2, pp 95–100 | Cite as

Human papillomavirus in breast cancer

  • Anna Di Lonardo
  • Aldo Venuti
  • Maria Luisa Marcante


Histological sections from paraffin-embedded breast carcinoma and axillary lymph nodes were examined for the presence of human papillomaviruses by two different techniques: the polymerase chain reaction (PCR) and thein situ hybridization with biotin-labelled probes. By PCR we detected HPV 16 DNA sequences in 29.4% of breast tumours and in some metastatic lymph nodes, though we were unable to identify any HPV DNA sequences byin situ hybridization. These results suggest that HPV's could play a role in the genesis of breast neoplasia.

Key words

breast cancer HPV in situ hybridization PCR 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Pfister H: Human papillomavirus and genital cancer (review). Adv Cancer Res 48: 113–147, 1987PubMedGoogle Scholar
  2. 2.
    Galloway DA, McDougall JK: Human papillomavirus and carcinomas. Adv Virus Res 37: 125–171, 1989PubMedGoogle Scholar
  3. 3.
    De Villiers EM: Heterogeneity of the human papillomavirus group. J Virol 63: 4898–4903, 1989PubMedGoogle Scholar
  4. 4.
    Dürst M, Gissmann L, Ikenberg H, Zur Hausen H: A papilloma virus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proc Natl Acad Sci USA 80: 3812–3815, 1983PubMedGoogle Scholar
  5. 5.
    Boshart M, Gissmann L, Ikenberg H, Kleinheinz A, Scheurlen W, Zur Hausen H: A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J 3: 1151–1157, 1984PubMedGoogle Scholar
  6. 6.
    Venuti A, Marcante ML: Presence of human papillomavirus type 18 DNA in vulvar carcinomas and its integration into the cell genome. J Gen Virol 70: 1587–1592, 1989PubMedGoogle Scholar
  7. 7.
    Pérez-Ayala M, Ruiz-Cabello F, Esteban F, Concha A, Redondo M, Oliva MR, Cabrera T, Garrido F: Presence of HPV 16 sequences in laryngeal carcinomas. Int J Cancer 46: 8–11, 1990PubMedGoogle Scholar
  8. 8.
    Scheurlen W, Stremlau A, Gissmann L, Höhn D, Zenner HP, Zur Hausen H: Rearranged HPV 16 molecules in an anal and in a laryngeal carcinoma. Int J Cancer 38: 671–676, 1986PubMedGoogle Scholar
  9. 9.
    Beckmann AM, Daling JR, Sherman KJ, Maden C, Miller BA, Coates RJ, Kiviat NB, Myerson D, Weiss NS, Hislop TG, Beagrie M, McDougall JK: Human papillomavirus infection and anal cancer. Int J Cancer 43: 1042–1049, 1989PubMedGoogle Scholar
  10. 10.
    Guitart J, Bergfeld WF, Tuthill RJ, Tubbs RR, Zienowicz R, Fleegler EJ: Squamous cell carcinoma of the nail bed: a clinicopathological study of 12 cases. Brit J Dermatol 123: 215–222, 1990Google Scholar
  11. 11.
    Pirisi L, Yasumoto S, Feller M, Doniger J, Di Paolo JA: Transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 DNA. J Virol 61: 1061–1066, 1987PubMedGoogle Scholar
  12. 12.
    Bedell MA, Jones KH, Laimins LA: The E6–E7 region of human papillomavirus type 18 is sufficient for transformation of NIH 3T3 and rat-1 cells. J Virol 61: 3635–3640, 1987PubMedGoogle Scholar
  13. 13.
    Kanda T, Furuno A, Yoshiike K: Human papillomavirus type 16 open reading frame E7 encodes a transforming gene for rat 3Y1 cells. J Virol 62: 610–613, 1988PubMedGoogle Scholar
  14. 14.
    Band V, Zajchowski D, Kulesa V, Sager R: Human papillomavirus DNAs immortalize normal human mammary epithelial cells and reduce their growth factor requirements. Proc Natl Acad Sci USA 87: 463–467, 1990PubMedGoogle Scholar
  15. 15.
    Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Ehrlich HA, Arnheim N: Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230: 1350–1354, 1985PubMedGoogle Scholar
  16. 16.
    Shibata DK, Arnheim N, Martin WJ: Detection of human papillomavirus in paraffin-embedded tissue using the polymerase chain reaction. J Exp Med 167: 225–230, 1988PubMedGoogle Scholar
  17. 17.
    Beckmann AM, Myerson D, Daling JR, Kiviat NB, Fenoglio CM, McDougall JK: Detection of human papillomavirus DNA in carcinomas by ‘in situ hybridization’ with biotinylated probes. J Med Virol 16: 265–273, 1985PubMedGoogle Scholar
  18. 18.
    Wells M, Griffiths S, Lewis F, Bird C: Demonstration of human papillomavirus types in paraffin processed tissue from human anogenital lesions byin situ DNA hybridization. J Pathology 152: 77–82, 1987Google Scholar
  19. 19.
    Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press, 1989Google Scholar
  20. 20.
    Marcante ML, Venuti A: Human papillomavirus DNA as a possible index of invasiveness in female genital tract carcinomas. Eur J Cancer 27: 187–190, 1991PubMedGoogle Scholar
  21. 21.
    Cornelissen MTE, Van Den Twell JG, Struyk APHB, Jebbink MF, Briët M, Van der Noordaa J, Ter Schegget J: Localization of human papillomavirus type 16 using the polymerase chain reaction in the cervix uteri of women with cervical intraepithelial neoplasia. J Gen Virol 70: 2555–2562, 1989PubMedGoogle Scholar
  22. 22.
    Johnson MA, Blomfield PI, Bevan IS, Woodman CBJ, Young LS: Analysis of human papillomavirus type 16 E6–E7 transcription in cervical carcinomas and normal cervical epithelium using the polymerase chain reaction. J Gen Virol 71: 1473–1479, 1990PubMedGoogle Scholar
  23. 23.
    Day PJR, Bevan JS, Gurney SJ, Young LS, Walker MR: Synthesisin vitro and application of biotinylated DNA probes for human papillomavirus type 16 by utilizing the polymerase chain reaction. Biochem J 267: 119–123, 1990PubMedGoogle Scholar
  24. 24.
    Muñoz N, Bosch FX, Kaldor JM: Does human papillomavirus cause cervical cancer? The state of the epidemiological evidence. Brit J Cancer 57: 1–5, 1988PubMedGoogle Scholar
  25. 25.
    Muñoz N, Bosch FX, Jensen OM: Human papillomavirus and cervical cancer. IARC Scientific Publications 94, 1989Google Scholar
  26. 26.
    Kowalzick L, Grimmel M, Weyer U, De Villiers EM, Janner M: Recurrent papillomas of the nipple associated with human papillomavirus 41. Br J Dermatol 122: 757–762, 1990PubMedGoogle Scholar
  27. 27.
    Walboomers JMM, Melchers WJG, Mullink H, Meijer CJLM, Struyk APHB, Quint WGJ, Van Der Noordaa J, Ter Schegget J: Sensitivity ofin situ detection with biotinylated probes of human papillomavirus type 16 DNA in frozen tissue sections of squamous cell carcinomas of the cervix. Am J Pathol 131: 587–594, 1988PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Anna Di Lonardo
    • 1
  • Aldo Venuti
    • 1
  • Maria Luisa Marcante
    • 1
  1. 1.Lab. of VirologyCRS, Regina Elena Institute for Cancer ResearchRomeItaly

Personalised recommendations