Modification of the mouse mammary gland organ culture technique
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A modified procedure for culturing an entire thoracic pair of mammary glands from virgin mice is described. The glands are incubated in CMRL 1066 medium supplemented with hormones in a controlled atmosphere chamber with a gas phase of 50% O2, 5% CO2, and 45% N2 at 37° C.
Key wordsmammary gland differentiation hormones
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