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Journal of tissue culture methods

, Volume 8, Issue 1, pp 27–30 | Cite as

Modification of the mouse mammary gland organ culture technique

  • R. G. Mehta
  • W. L. Cerny
  • M. J. Madigan
  • R. C. Moon
Article

Summary

A modified procedure for culturing an entire thoracic pair of mammary glands from virgin mice is described. The glands are incubated in CMRL 1066 medium supplemented with hormones in a controlled atmosphere chamber with a gas phase of 50% O2, 5% CO2, and 45% N2 at 37° C.

Key words

mammary gland differentiation hormones 

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Copyright information

© Tissue Culture Association, Inc. 1984

Authors and Affiliations

  • R. G. Mehta
    • 1
  • W. L. Cerny
    • 1
  • M. J. Madigan
    • 1
  • R. C. Moon
    • 1
  1. 1.Laboratory of PathophysiologyLife Sciences Research Division IIT Research InstituteChicagoUSA

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