Journal of tissue culture methods

, Volume 8, Issue 1, pp 27–30 | Cite as

Modification of the mouse mammary gland organ culture technique

  • R. G. Mehta
  • W. L. Cerny
  • M. J. Madigan
  • R. C. Moon


A modified procedure for culturing an entire thoracic pair of mammary glands from virgin mice is described. The glands are incubated in CMRL 1066 medium supplemented with hormones in a controlled atmosphere chamber with a gas phase of 50% O2, 5% CO2, and 45% N2 at 37° C.

Key words

mammary gland differentiation hormones 


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  1. 1.
    Topper, Y. J.; Freeman, C. S. Multiple hormone interactions in the developmental biology of the mammary gland. Physiol. Rev. 60: 1049–1106; 1980.PubMedGoogle Scholar
  2. 2.
    Ichinose, R. R.; Nandi, S. Influence of hormones on lobuloalveolar differentiation of mouse mammary glandsin vitro. J. Endocrinol. 35: 331–340; 1966.PubMedGoogle Scholar
  3. 3.
    Mehta, R. G. Hormonal regulation of lobuloalveolar development and macromolecular biosynthesis of the entire mammary gland in organ culture. Lincoln, NE: Univ. of Nebraska; 1974. Thesis.Google Scholar
  4. 4.
    Mehta, R. G.; Banerjee, M. R. Action of growth promoting hormones on macromolecular biosynthesis during lobuloalveolar development of the entire mammary gland in organ culture. Acta Endocrinol. 80: 501–516; 1975.PubMedGoogle Scholar
  5. 5.
    Wood, B. G.; Washburn, L. L.; Mukherjee, A. S.; Banerjee, M. R. Hormonal regulation of lobuloalveolar growth, functional differentiation and regression of mouse mammary gland in organ culture. J. Endocrinol. 65: 1–6; 1975.PubMedGoogle Scholar
  6. 6.
    Lin, F. K.; Banerjee, M. R.; Crump, L. R. Cell cycle-related hormone carcinogen interaction during chemical carcinogen induction of nodule-like mammary lesions in organ culture. Cancer Res. 36: 1607–1614; 1976.PubMedGoogle Scholar
  7. 7.
    Telang, N. T.; Banerjee, M. R.; Iyer, A. P.; Kundu, A. B. Neoplastic transformation of epithelial cells in whole mammary glandin vitro. Proc. Natl. Acad. Sci. USA 76: 5886–5890; 1979.PubMedGoogle Scholar
  8. 8.
    Banerjee, M. R.; Wood, B. G.; Lin, F. K.; Crump, L. R. Organ culture of whole mammary gland of the mouse. TCA Manual 2: 457–462; 1976.CrossRefGoogle Scholar
  9. 9.
    Mehta, R. G.; Cerny, W. L.; Moon, R. C. Retinoids inhibit prolactin induced differentiation of the mammary glandin vitro. Carcinogenesis 4: 23–26.Google Scholar
  10. 10.
    Mehta, R. G.; Moon, R. C. Retinoid metabolism and retinoic acid binding protein in mammary glandin vitro. The Endocrine Society 65th Annual Meeting; San Antonio, TX.; 1983: 165.Google Scholar

Copyright information

© Tissue Culture Association, Inc. 1984

Authors and Affiliations

  • R. G. Mehta
    • 1
  • W. L. Cerny
    • 1
  • M. J. Madigan
    • 1
  • R. C. Moon
    • 1
  1. 1.Laboratory of PathophysiologyLife Sciences Research Division IIT Research InstituteChicagoUSA

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