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Modification of the mouse mammary gland organ culture technique

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Journal of tissue culture methods

Summary

A modified procedure for culturing an entire thoracic pair of mammary glands from virgin mice is described. The glands are incubated in CMRL 1066 medium supplemented with hormones in a controlled atmosphere chamber with a gas phase of 50% O2, 5% CO2, and 45% N2 at 37° C.

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Authors and Affiliations

  1. Laboratory of Pathophysiology, Life Sciences Research Division IIT Research Institute, 60616, Chicago, Illinois, USA

    R. G. Mehta, W. L. Cerny, M. J. Madigan & R. C. Moon

Authors
  1. R. G. Mehta
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  2. W. L. Cerny
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  3. M. J. Madigan
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  4. R. C. Moon
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Mehta, R.G., Cerny, W.L., Madigan, M.J. et al. Modification of the mouse mammary gland organ culture technique. Journal of Tissue Culture Methods 8, 27–30 (1983). https://doi.org/10.1007/BF01834631

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  • DOI: https://doi.org/10.1007/BF01834631

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