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Quality of pooled platelet concentrates prepared from buffy coats and stored in an additive solution after filtration

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Abstract

Platelet concentrates prepared from buffy coat were pooled and stored for 6 days after removal of leukocytes by filtration. The platelets were stored in plasma or in an additive solution, Plasmalyte-A. In vitro platelet function was better preserved using Plasmalyte-A than plasma with regard to osmotic reversal and aggregation. No significant differences for the release of platelet markersΒ-thromboglobulin, platelet factor 4, or lactate dehydrogenase pre- and post-filtration and storage in plasma or Plasmalyte-A was observed. Expression of the surface membrane glycoproteins Ib, Ia/ IIa, Ilb/IIIa, and IV measured by flow cytometry after binding of monoclonal antibodies did not change during storage. The expression of activation-dependentα- granula glycoprotein GMP140, the thrombospondin, and the glycoprotein 53 from the lysosomal granules was not different between platelet pools stored in plasma or in Plasmalyte-A. The in vitro quality of platelets stored as pools is comparable for plasma and the additive solution Plasmalyte-A.

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References

  1. Adams GA, Rock G (1988) Storage of human platelet concentrates in an artificial medium without dextrose. Transfusion 28:217–220

    PubMed  Google Scholar 

  2. Bertolini F, Rebulla P, Riccardi D, Cortellaro M, Ranzi ML, Sirchia G (1989) Evaluation of platelet concentrates prepared from buffy coats and stored in a glucose-free crystalloid medium. Transfusion 29:605–609

    PubMed  Google Scholar 

  3. Bertolini F, Murphy S, Sirchia G (1992) Role of acetate during platelet storage in a synthetic medium. Transfusion 32:152–166

    PubMed  Google Scholar 

  4. Bertolini F, Rebulla P, Marangoni F, Sirchia G (1992) Plate-let concentrates stored in synthetic medium after filtration. Vox Sang 62:82–86

    PubMed  Google Scholar 

  5. Boomgaard MD, Gouwerok CWN, de Korte C, Loos JA (1994) Platelets stored for 6 days in a polyolefin container in a synthetic medium with minimal plasma carry-over. Vox Sang 66:18–24

    PubMed  Google Scholar 

  6. DiMinno G, Silver MJ, Murphy S (1982) Stored human plate-lets retain full aggregation potential in response to pairs of aggregating agents. Blood 59:563–568

    PubMed  Google Scholar 

  7. Fijnheer R, Modderman PW, Veldman H, Ouwehand WH, Nieuwenhuis HK, Roos D, de Korte D (1990) Detection of platelet activation with monoclonal antibodies and flow cytometry. Transfusion 30:20–25

    PubMed  Google Scholar 

  8. Fijnheer R, Veldman HA, van den Eertwegh AJM, Gouwerok CWN, Homburg CHE, Boomgaard MN, de Korte D, Roos D (1991) In vitro evaluation of buffy-coat-derived platelet concentrates stored in a synthetic medium. Vox Sang 60:16–22

    PubMed  Google Scholar 

  9. George JN (1987) The role of membrane glycoproteins in platelet function. Transfus Med Rev 1:34–46

    PubMed  Google Scholar 

  10. George JN, Pickett EB, Heinz R (1988) Platelet membrane glycoprotein changes during the preparation and storage of platelet concentrates. Transfusion 28:123–126

    PubMed  Google Scholar 

  11. Goodall AH (1991) Platelet activation during preparation and storage of concentrates: detection by flow cytometry. Blood Coagulation Fibrinolysis 2:377–382

    PubMed  Google Scholar 

  12. Gulliksson H, Sallander S, Pedajas I, Christenson M, Wiechel B (1992) Storage of platelets in additive solutions: a new method for storage using sodium chloride solution. Transfusion 32:435–440

    PubMed  Google Scholar 

  13. Heal JM, Singal S, Sardisco E, Mayer T (1986) Bacterial proliferation in platelet concentrates. Transfusion 26:388–390

    PubMed  Google Scholar 

  14. Holme S (1992) Effect of additive solutions on platelet biochemistry. Blood Cells 18:421–430

    PubMed  Google Scholar 

  15. Kim BK, Baldini MG (1974) The platelet response to hypotonic shock. Its value as an indicator of platelet viability after storage. Transfusion 14:130–138

    PubMed  Google Scholar 

  16. Koerner K (1983) Platelet function after shipment of roomtemperature platelet concentrates. Vox Sang 44:37–41

    PubMed  Google Scholar 

  17. Koerner K (1984) Platelet function of room-temperature platelet concentrates stored in a new plastic material with high gas permeability. Vox Sang 47:406–411

    PubMed  Google Scholar 

  18. Koerner K, Sahlmen P, Zimmermann B, Cardoso M, Kubanek B (1994) In vitro platelet function during storage in three different additive solutions. Vox Sang 67:154–159

    PubMed  Google Scholar 

  19. Masse M, Naegelen C, Pellegrini N, Segier JM, Marpaux N, Beaujean F (1992) Validation of a simple method to count very low white cell concentrations in filtered red cells or platelets. Transfusion 32:565–571

    PubMed  Google Scholar 

  20. Michelson AD, Barnard MR (1990) Plasmin-induced redistribution of platelet glycoprotein Ib. Blood 76:2005–2010

    PubMed  Google Scholar 

  21. Moroff G, Holme S, Dabay MH, Sawyer S, Heaton WAL, Law P, Alsop P (1993) Storage of pools of six and eight platelet concentrates. Transfusion 33:374–378

    PubMed  Google Scholar 

  22. Murphy S, Sayar SN, Gardner FH (1979) Storage of platelet concentrates at 22‡ C. Blood 35:549–557

    Google Scholar 

  23. Muylle L, Petermans ME (1994) Effect of prestorage leukocyte removal on the cytokine levels in stored platelet concentrates. Vox Sang 66:14–17

    PubMed  Google Scholar 

  24. Pietersz RN, Reesink HW, Dekker WJA, Fijen FJ (1987) Preparation of leukocyte-poor platelet concentrates from buffy coats. I. Special inserts for centrifuge cups. Vox Sang 53:203–207

    PubMed  Google Scholar 

  25. Rinder HM, Murphy M, Mitchell JG, Stocks J, Ault KA, Hillman RS (1991) Progressive platelet activation with storage: evidence for shortened survival of activated platelets after transfusion. Transfusion 31:409–414

    PubMed  Google Scholar 

  26. Rinder HM, Snyder EL (1992) Activation of platelet concentrate during preparation and storage. Blood Cells 18:445–456

    PubMed  Google Scholar 

  27. Sloand EM, Klein HG (1990) Effect of white cells on platelets during storage. Transfusion 30:333–338

    PubMed  Google Scholar 

  28. Snyder EL, Hezzey A, Katz AJ, Bock J (1981) Occurrence of the release reaction during preparation and storage of platelet concentrates. Vox Sang 41:172–177

    PubMed  Google Scholar 

  29. Snyder EL, Stack G, Napychank P, Robert S (1989) Storage of pooled platelet concentrates: in vitro and in vivo analysis. Transfusion 20:390–395

    Google Scholar 

  30. Taylor MA, Anstee DJ (1984) The use of functional and quantitative assays to study glycoprotein Ib in platelets stored under various in vitro conditions. Thromb Haemost 52:271–275

    PubMed  Google Scholar 

  31. Triulzi DJ, Kickler TS, Braine HG (1992) Detection and significance of alpha granule membrane protein 140 expression on platelets collected by apheresis. Transfusion 32:529–533

    PubMed  Google Scholar 

  32. Tschöpe D, Schauseil S, Roesen P, Kaufmann L, Gries FA (1988) Flow-cytometric determination of platelet membranebound glycoproteins. Klin Wochenschr 66:117–122

    Google Scholar 

  33. Wun T, Paglieroni T, Sazama K, Holland P (1992) Detection of plasmapheresis-induced platelet activation using monoclonal antibodies. Transfusion 32:534–540

    PubMed  Google Scholar 

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Authors and Affiliations

  1. German Red Cross Blood Bank, Helmholtzstrasse 10, D-89081, Ulm, Germany

    K. Koerner, P. Sahlmen, B. Zeller, M. Cardoso & B. Kubanek

  2. Department of Hemostaseology, University of Ulm, Germany

    R. Weihe

  3. German Red Cross Blood Bank, Frankfurt/M, Germany

    E. Seifried

Authors
  1. K. Koerner
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  2. R. Weihe
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  3. P. Sahlmen
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  4. B. Zeller
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  5. E. Seifried
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  6. M. Cardoso
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  7. B. Kubanek
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Additional information

Part of this work (R.W.) served as requirement for the degree of M.D. at the University of Ulm

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Koerner, K., Weihe, R., Sahlmen, P. et al. Quality of pooled platelet concentrates prepared from buffy coats and stored in an additive solution after filtration. Ann Hematol 70, 97–102 (1995). https://doi.org/10.1007/BF01834388

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  • DOI: https://doi.org/10.1007/BF01834388

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