Abstract
We evaluated three solutions used for preserving lungs, namely, University of Wisconsin (UW), Euro-Collins (E-C), and low potassium dextran (LPD), by measuring the high energy phosphates in the preserved lung tissue. The left lungs of Sprague-Dawley rats were excised and flushed with 5 ml of one of the solutions at 10°C through the pulmonary artery, after which they were deflated and immersed in the solution at 10°C for 24 h. The tissue adenosine triphosphate (ATP) concentration in μmol/g tissue wet weight after 24 h of storage was 2.55 ± 0.48 (n = 7) in the UW lungs, 1.98 ± 0.25 (n = 6) in the E-C lungs, and 1.53 ± 0.32 (n = 4) in the LPD lungs, being significantly higher in the UW lungs than in either the E-C or LPD lungs (P < 0.05). The histopathological findings of the E-C lungs were more deteriorated, with marked interstitial edema, septal hypertrophy, and perivascular hyaline degeneration, than either the UW or LPD lungs. Thus, the findings of this study indicate the superiority of UW solution for lung preservation.
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Ploeg RJ, Goossens D, McAnulty JF, Southard JH, Belzer FO (1988) Successful 72-hour cold storage of dog kidneys with UW solution. Transplantation 46:191–196
Wahlberg JA, Love R, Landegaard L, Southard JH, Belzer FO (1987) 72-hour preservation of the canine pancreas. Transplantation 43:5–8
Ploeg RJ, Goossens D, Sollinger HW, Southard JH, Belzer FO (1988) Efficacy of 48-hour pancreas preservation with UW solution in the dog allograft model. Transplant Proc 20:1026–1028
Jamieson NV, Sundberg R, Lindell S, Southard JH, Belzer FO (1988) The isolated perfused rabbit liver as a model for assessment of organ preservation. Transplant Proc 20:996–997
Swanson DK, Pasaoglu I, Berkoff HA, Southard JA, Hegge JO (1988) Improved heart preservation with UW preservation solution. J Heart Transplant 7:456–467
Maurer EJ, Swanson DK, DeBoer LWV (1990) Comparison of UW and Collins solution for preservation of the rat heart. Transplant Proc 22:548–550
Kawahara K, Ikari H, Hisano H, Takahashi T, Honshou S, Ayabe H, Tomita M (1991) Twenty-four-hour canine lung preservation using UW solution. Transplantation 51:584–587
Fujimura S, Kondo T, Handa M, Yamauchi A, Okabe T, Shionozaki F, Nakada T (1985) Successful 24-hour preservation of canine lung transplants using modified extracellular fluid. Transplant Proc 17:1466–1467
Fujimura S, Handa M, Kondo T, Ichinose T, Shiraishi Y, Nakada T (1987) Successful 48-hour simple hypothermic preservation of canine lung transplants. Transplant Proc 19:1334–1336
Yamazaki F, Yokomise H, Keshavjee SH, Miyoshi S, Cardoso PF, Slutsky AS, Patterson GA (1990) The superiority of an extracellular fluid solution over Euro-Collins solution for pulmonary preservation. Transplantation 49:690–694
Calhoon JH, Grover FL, Gibbons WJ, Bryan CL, Levine SM, Bailey SR, Nichols L, Lum C, Trinkle JK (1991) Single lung transplantation. J Thorac Cardiovasc Surg 101:816–825
Said SI, Foda HD (1989) Pharmacologic modulation of lung injury. Am Rev Resir Dis 139:1553–1564
Puskas JD, Cardoso PFG, Mayer E, Shi S, Slutsky AS, Patterson GA (1992) Equivalent eighteen-hour lung preservation with low-potassium dextran or Euro-Collins solution after prostaglandin El infusion. J Thorac Cardiovasc Surg 104:83–89
Swanson DK, Dufek JH, Barber TA, Karn DR (1979) Improving function of hearts preserved for 24 hours by controlling reperfusion. Transplantation 28:476–481
Belzer FO, Southard JH (1988) Principles of solid-organ preservation by cold storage. Transplantation 45:673–676
Weder W, Harper B, Shimokawa S, Miyoshi S, Date H, Schreinemakers H, Egan T, Cooper JD (1991) Influence of intraalveolar oxygen concentration on lung preservation in a rabbit model. J Thorac Cardiovasc Surg 101:1037–1043
Southard JH, Van Gulik TM, Ametani MS, Vreugdenhil PK, Lindell SL, Pienaar BL, Belzer FO (1990) Important components of the UW solution. Transplantation 49:251–257
Wang LS, Yoshikawa K, Miyoshi S, Nakamoto K, Hsieh CM, Yamazaki F, Guerreiro Cardoso PF, Schaefers GHJ, Brito J, Keshavjee SH, Patterson A, Cooper JD (1989) The effect of ischemic time and temperature on lung preservation in a simple ex vivo rabbit model used for functional assessment. J Thorac Cardiovasc Surg 98:333–342
Date H, Lima O, Matsumura A, Tsuji H, d'Avignon DA, Cooper JD (1992) In a canine model, lung preservation at 10°C is superior to that at 4°C: a comparison of two preservation temperatures on lung function and on adenosine triphosphate level measured by phosphoros 31-nuclear magnetic resonance. J Thorac Cardiovasc Surg 103:773–780
Sumimoto R, Jamieson NV, Kamada N (1990) Examination of the role of the impermeants lactobionate and raffinose in a modified UW solution. Transplantation 50:573–576
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Omote, Y., Ikeda, K., Yamazaki, K. et al. The superiority of UW solution for maintaining ATP concentrations during pulmonary preservation. Surg Today 24, 616–620 (1994). https://doi.org/10.1007/BF01833726
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DOI: https://doi.org/10.1007/BF01833726