Clinical & Experimental Metastasis

, Volume 9, Issue 1, pp 13–25 | Cite as

Effect of catechins and citrus flavonoids on invasionin vitro

  • M. Bracke
  • B. Vyncke
  • G. Opdenakker
  • J. -M. Foidart
  • G. De Pestel
  • M. Mareel


Catechins, a group of flavonoid molecules, inhibit invasion of mouse MO4 cells into embryonic chick heart fragmentsin vitro. The anti-invasive effects can be ranked as follows: (+)-catechin > (−)-epicatechin>3-O-methyl-(+)-catechin > 3-O-palmitoyl-(+)-catechin. Most of the catechins are unstable in cell culture media, and their spontaneous rearrangement products tend to bind to extracellular matrix (ECM). Due to these interactions proteases such as tissue-type plasminogen activator (t-PA) are linked to the ECM glycoprotein laminin. This leads to a partial inactivation of the enzyme. Within the group of catechins we found a positive correlation between anti-invasive activity and linking of t-PA to laminin. Citrus flavonoids are also anti-invasivein vitro (tangeretin > nobiletin > hesperidin = naringin). However, these stable molecules show poor affinity for ECM, and do not link enzymes to laminin. These data suggest that catechins and citrus flavonoids inhibit invasionin vitro by different mechanisms.


Laminin Catechin Epicatechin Naringin Hesperidin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Rapid Communications of Oxford Ltd 1991

Authors and Affiliations

  • M. Bracke
    • 1
  • B. Vyncke
    • 1
  • G. Opdenakker
    • 2
  • J. -M. Foidart
    • 3
  • G. De Pestel
    • 4
  • M. Mareel
    • 1
  1. 1.Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear MedicineUniversity HospitalGentBelgium
  2. 2.Rega Institute for Medical ResearchUniversity of LeuvenBelgium
  3. 3.Laboratory of Dermatology and Department of Gynecology and ObstetricsUniversity of LiègeBelgium
  4. 4.Laboratory of EmbryologyUniversity of GentBelgium

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