, Volume 10, Issue 2, pp 116–119 | Cite as

Bacterial adherence to urothelium following bladder irrigation in the rat

  • P. Iversen
  • P. O. Madsen


We investigated the effects of bladder irrigation with various irrigation fluids at various temperatures and irrigation pressures on the adherence of3H-labeledEscherichia coli O6 to bladder urothelium in a rat model. Sixty-four control and 108 experimental animals were studied. The latter were divided into five groups according to type of irrigation fluid (glycine 1.5%, sorbitol 2.7%, mannitol 0.54%, and H2O), fluid temperature, and intravesical pressure during irrigation. Only rats undergoing high pressure (60 cm H2O) bladder irrigation demonstrated significant increases in bacterial adherence compared to controls. No differences were found with the different irrigation fluids. This study suggests that high intravesical pressure during transurethral surgery might enhance bacterial adherence to urothelium and thereby increase the risk of manifest infection in the postoperative course.


Glycin Mannitol Family Medicine Sorbitol Fluid Temperature 
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Adhärenz von Bakterien an Urothel nach Blasenspülung bei der Ratte


In einem Rattenmodell wurde der Einfluß der Blasenspülung mit verschiedenen Spülflüssigkeiten bei unterschiedlichen Temperaturen und Spüldrucken auf die Haftfähigkeit von3H-markiertenEscherichia coli O6 Urothel der Blase untersucht. Die Studie wurde an 64 Kontrolltieren und 108 behandelten Tieren durchgeführt. In der experimentellen Gruppe erfolgte eine Unterteilung in fünf Untergruppen entsprechend der Art der Spülflüssigkeit (1,5%iges Glycin, 2,7%iges Sorbit, 0,54%iges Mannit und H2O), Temperatur der Flüssigkeit und intravesikalem Druck während der Spülung. Nur bei Ratten, die einer Blasenspülung unter hohem Druck (60 cm H2O) ausgesetzt waren, zeigte sich ein signifikanter Anstieg in der bakteriellen Adhärenz im Vergleich zu den Kontrolltieren. Die verschiedenen Spülflüssigkeiten führten zu keinen Unterschieden in der Adhärenz. Aus den Ergebnissen der Studie kann angenommen werden, daß hohe intravesikale Drucke während transurethraler chirurgischer Eingriffe die Haftfähigkeit von Bakterien am Urothel verstärken und dadurch das Risiko einer manifesten Infektion in der postoperativen Phase verstärken.


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© MMW Medizin Verlag München GmBH 1982

Authors and Affiliations

  • P. Iversen
    • 1
  • P. O. Madsen
    • 1
  1. 1.Urology Section, William S. Middleton Memorial Veterans Hospital and Department of SurgeryUniversity of Wisconsin School of MedicineMadisonUSA

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