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Growth kinetics of four human breast carcinomas grown in nude mice

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Summary

The immune-deficient nude mouse with human tumor xenografts is an appropriate model system for performing detailed growth kinetic examinations. In the present study one estrogen and progesterone receptor-negative (T60) and three receptor-positive (Br-10, MCF-7, T61) human breast cancer xenografts in nude mice were investigated. The proliferative tumor characteristics were examined by growth curves, thymidine labelling technique, and flow cytometric DNA analysis performed on fine-needle aspirations. The results showed that the tumors had growth kinetics comparable to other human tumor types with cell generation times of 42 to 60 hours. The three receptor-positive tumors had slower growth rate, larger tumor volume doubling time, and smaller growth fraction and labelling index than the receptor-negative tumor. However, no single proliferation parameter was sufficient to characterize the growth kinetics of individual tumors or to describe proliferative differences between the tumors.

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Authors and Affiliations

  1. University Institute of Pathological Anatomy, Frederik V's Vej 11, DK-2100, Copenhagen, Denmark

    M. Spang-Thomsen, K. Rygaard, L. Hansen, A. -C. Halvorsen & N. Brünner

  2. Finsen Institute, Copenhagen, Denmark

    L. L. Vindeløv

Authors
  1. M. Spang-Thomsen
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  2. K. Rygaard
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  3. L. Hansen
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  4. A. -C. Halvorsen
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  5. L. L. Vindeløv
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  6. N. Brünner
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Spang-Thomsen, M., Rygaard, K., Hansen, L. et al. Growth kinetics of four human breast carcinomas grown in nude mice. Breast Cancer Res Tr 14, 235–243 (1989). https://doi.org/10.1007/BF01810740

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