Advertisement

Clinical & Experimental Metastasis

, Volume 8, Issue 4, pp 381–389 | Cite as

Influence of tumor transplantation methods on tumor growth rate and metastatic potential of solitary tumors derived from metastases

  • J. P. G. Volpe
  • L. Milas
Article

Abstract

This study was performed to determine whether the growth rate and metastatic potential of tumors generated by spontaneous lung metastases is influenced by transplantation methods. Three different tumors syngeneic to C3Hf/Kam mice were studied: the SA-NH and SA-4020 sarcomas and the hepatocarcinoma HCA-I. Solitary tumors in the legs of mice were generated by a single metastatic nodule taken at random from lung metastases, by a single metastatic nodule taken from each mouse with the highest number of metastases, by a mixture of cells from lung metastases taken randomly, or by a mixture of cells from primary leg tumors. These transplantation procedures were repeated for two to four isotransplant generations. Repeated isotransplants of primary tumors showed little if any change in the growth rate and metastatic spread. In contrast, primary tumors derived from spontaneous metastases frequently exhibited a decrease in their growth rate and an increase in metastatic potential. This was particularly frequent when tumors were established from single metastatic nodules taken randomly from the lung, or taken from lungs that contained the largest number of metastatic nodules. The magnitude of this change varied greatly among the three tumors studied. Increased metastatic formation in the lung was also frequently associated with slower growth of the primary tumors. Thus, transplantation methods used for establishing primary tumors have an important influence on the metastatic potential of tumor transplants.

Keywords

Growth Rate Primary Tumor Sarcoma Lung Metastasis Metastatic Potential 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Barut, B. A., andKlaunic, J. E., 1986, Isolation and characterization of metastic sublines from a murine transitional cell bladder carcinoma.Clinical and Experimental Metastasis,4, 1–11.Google Scholar
  2. [2]
    Battazzi, B.,Mantovani, A.,Taraboletti, G., andGiavazzi, R., 1986, Characterization of spontaneous metastases from autochthonous 3-methylcholanthrene-induced tumors.Invasion and Metastasis,6, 44–57.Google Scholar
  3. [3]
    Eccles, S. A.,Heckford, S. E., andAlexander, P., 1980, Effect of cyclosporin A on the growth and spontaneous metastasis of syngeneic animal tumors.British Journal of Cancer,42, 252–259.PubMedGoogle Scholar
  4. [4]
    Giavazzi, R.,Alessandri, G.,Spreafico, F.,Garattini, S., andMantovani, A., 1980, Metastasizing capacity of tumor cells from spontaneous metastases of transplanted murine tumors. British Journal of Cancer, 42, 462–472.PubMedGoogle Scholar
  5. [5]
    Isaacs, J., 1981, Mechanism for and implications of the development of heterogeneity of androgen sensitivity in prostatic cancer.Tumor Cell Heterogeneity: Origins and Implications, edited by A. H. Owens, D. S. Coffey and S. B. Baylin (Orlando, FL: Academic Press), pp. 99–114.Google Scholar
  6. [6]
    Koch, F. E., 1939, Zur Frage der Metastasenbildung bei Impftumoren.Zeitschrift fur Krebsforschung,48, 495–505.Google Scholar
  7. [7]
    Lapeyriere, O. D.,Arnaud, D.,Courcoul, M.,Planche, J.Meyer, G., andBirg, F., 1986, Polyomavirus-transformed FR373 rat cells are able to form metastases in syngeneic rats.Virology,148, 146–158.PubMedGoogle Scholar
  8. [8]
    Layton, M. G. andFranks, L. M., 1984, Heterogeneity in a spontaneous mouse lung carcinoma: selection and characterization of stable metastatic variants.British Journal of Cancer,49, 415–421.PubMedGoogle Scholar
  9. [9]
    Maguida, M.,Whur, P.,Lockwood, J.,Boston, J., andWilliams, D. C., 1980, Lewis lung carcinoma: selecting metastatic variants.Metastasis: Clinical and Experimental Aspects, edited by K. Hellmann,P. Hilgard and S. Eccles (The Hague: Martinus Nijhoff), pp. 179–183.Google Scholar
  10. [10]
    Mantovani, A.,Giavazzi, R.,Alessandri, G.,Spreafico, F., andGarattini, S., 1981, Characterization of tumor lines derived from spontaneous metastases of a transplanted murine sarcoma.European Journal of Cancer,17, 71–76.PubMedGoogle Scholar
  11. [11]
    Milas, L.,Peters, L. J., andIto, H., 1983, Spontaneous metastasis, random or selective?Clinical and Experimental Metastasis,1, 309–315.PubMedGoogle Scholar
  12. [12]
    Milas, L.,Hirata, H.,Hunter, N., andPeters, L. J., 1988, Effect of radiation-induced injury of tumor bed stroma on metastatic spread of murine sarcomas and carcinomas.Cancer Research, 48, 2116–2120.PubMedGoogle Scholar
  13. [13]
    Nanni, P.,De Giovanni, C.,Lollini, P.,Nicoletti, G., andProdi, G., 1986, Clones with different metastatic capacity and variant selection during metastasis: a problematic relationship.Journal of the National Cancer Institute, 76, 87–93.PubMedGoogle Scholar
  14. [14]
    Neri, A.,Welch, D.,Kawaguchi, T., andNicolson, G. L., 1982, Developmental and biologic properties of malignant cell sublines and clones of spontaneously metastasizing rat mammary adenocarcinoma.Journal of the National Cancer Institute,68, 507–517.PubMedGoogle Scholar
  15. [15]
    Novle, R. L., andHoover, L., 1975, A classification of transplantable tumors in N6 rats controlled by estrogen from dormancy to autonomy.Cancer Research,35, 2935–2941.PubMedGoogle Scholar
  16. [16]
    PRICE, J. E.,Aukerman, S. L., andFidler, I. J., 1986, Evidence that the process of murine melanoma metastasis is sequential and selective and contains stochastic elements.Cancer Research,46, 5172–5178.Google Scholar
  17. [17]
    Risely, G. P., andSherbet, G. V., 1987, Loss of glucocorticoid receptors in B16BL6 murine melanoma associated with serial transplantation, metastatic selection and altered growth properties.Clinical and Experimental Metastasis,5, 301–310.PubMedGoogle Scholar
  18. [18]
    Talmadge, J. E., 1983, The selective nature of metastasis.Cancer Metastasis Reviews,2, 25–40.PubMedGoogle Scholar
  19. [19]
    Talmadge, J. E., andFidler, I. J., 1982, Cancer metastasis is selective or random depending on the parent tumor population.Nature,297, 593–594.PubMedGoogle Scholar
  20. [20]
    Volpe, J. P. G., 1989, Inherited and noninherited sources of variation in metastasis and the growth rate of tumors: implications for tumor evolution and therapy. Ph.D. dissertation, University of Texas Health Science Center at Houston, Graduate School of Biomedical Sciences.Google Scholar
  21. [21]
    Volpe, J. P.,Hunter, N., andMilas, L., 1988, Metastatic instability of murine tumor metastases: dependence on tumor type.Clinical and Experimental Metastasis,6, 333–346.PubMedGoogle Scholar
  22. [22]
    Vaage, J., 1989, Loss of spontaneous metastasizing potential in mouse mammary tumors.Clinical and Experimental Metastasis,7, 373–378.PubMedGoogle Scholar
  23. [23]
    Wake, N.,Isaacs, J., andSandberg, A. A., 1982, Chromosomal changes associated with progression of the Dunning R-3327 rat prostatic adenocarcinoma system.Cancer Research, 42, 4131–4142.Google Scholar
  24. [24]
    Weiss, L.,Holmes, J. C., andWard, P. M., 1983, Do metastases arise from pre-existing subpopulations of cancer cells?British Journal of Cancer,47, 81–89.PubMedGoogle Scholar

Copyright information

© Taylor & Francis Ltd. 1990

Authors and Affiliations

  • J. P. G. Volpe
    • 1
  • L. Milas
    • 1
  1. 1.Department of Experimental RadiotherapyUniversity of Texas M. D. Anderson Cancer CenterHoustonUSA

Personalised recommendations