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Role of polyamines in the synthesis of prolactin-regulated growth factors by experimental breast cancer in culture

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Summary

The present experiments were designed to test whether polyamines play an essential role in the synthesis of growth factors induced by ovine prolactin (oPRL), using the N-nitrosomethylurea (NMU)-induced rat mammary tumor cultured in the soft agar clonogenic assay. Conditioned media (CM) obtained from tumors treated with oPRL and the polyamine biosynthesis inhibitor α-difluoromethylornithine (DFMO) (1 mM) no longer exerted the colony-stimulating effect which was observed with oPRL-CM. Such growth-promoting activity was restored with conditioned media obtained from tumors treated with oPRL, DFMO, and increasing concentrations of spermidine from 1 to 500µM. The colony-stimulating effect of the CM employed could not be accounted for by the contaminating presence in the media of oPRL, DFMO, and polyamines. These results indicate that in our system polyamines play an important role in the synthesis of oPRL-regulated growth factors.

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References

  1. Manni A, Pontari M, Wright C: Autocrine stimulation by prolactin hormone-responsive breast cancer growth in culture. Endocrinology 117: 2040–2043, 1985

    PubMed  Google Scholar 

  2. Manni A, Wright C, Hsu C-J, Hammond JM: Polyamines and autocrine control of tumor growth by prolactin in experimental breast cancer in culture. Endocrinology 119: 2033–2037, 1986

    PubMed  Google Scholar 

  3. Arafah BM, Finegan HM, Roe J, Manni A, Pearson OH: Hormone dependency in N-nitrosomethylurea-induced rat mammary tumors. Endocrinology 111: 584–588, 1982

    PubMed  Google Scholar 

  4. Manni A, Wright C: Polyamines as mediators of the effect of prolactin and growth hormone on the growth of N-nitroso-N-methylurea-induced rat mammary tumor culturedin vitro in soft agar. J Natl Cancer Inst 74: 941–944, 1985

    PubMed  Google Scholar 

  5. Manni A, Wright C: Effect of tamoxifen and α-difluoromethylornithine on clones of nitrosomethylurea-in-duced rat mammary tumor cells grown in soft agar. Cancer Res 43: 1084–1086, 1983

    Google Scholar 

  6. Manni A, Wright C: Polyamines as mediators of estrogen action on the growth of experimental breast cancer in rats. J Natl Cancer Inst 73: 511–514, 1984

    PubMed  Google Scholar 

  7. Manni A, Wright C: Reversal of the antiproliferative effect of the antiestrogen tamoxifen by polyamines in breast cancer cells. Endocrinology 114: 836–839, 1984

    PubMed  Google Scholar 

  8. Luk GD, Baylin SD: Inhibition of intestinal epithelial DNA synthesis and adaptive hyperplasia after jejunectomy in the rat by suppression of polyamine biosynthesis. J Clin Invest 74: 698–704, 1984

    PubMed  Google Scholar 

  9. Grove J, Fozard JR, Mamont PS: Assay of α-difluoromethyl-ornithine in body fluids and tissues by automatic aminoacid analysis. J Chromatogr 223: 409–416, 1981

    PubMed  Google Scholar 

  10. Winer BJ: Statistical Principles in Experimental Design. New York: McGraw-Hill, New York, 1971, pp 185–196

    Google Scholar 

  11. Manni A, Wright C, Feil P, Baranao L, Demers L, Garcia M, Rochefort H: Autocrine stimulation by estradiol-regulated growth factors of rat hormone-responsive mammary cancer: Interaction with the polyamine pathway. Cancer Res 46: 1594–1598, 1986

    Google Scholar 

  12. Chalbos D, Vignon F, Keydar I, Rochefort H: Estrogens stimulate cell proliferation and induce secretory proteins in human breast cancer cell line (T47D). J Clin Endocrinol Metab 55: 276–283, 1982

    PubMed  Google Scholar 

  13. Vignon F, Lippman ME, Nawata H, Derocq D, Rochefort H: Induction of two estrogen-responsive proteins by antiestrogens in R27, a tamoxifen-resistant clone of MCF-7 cells. Cancer Res 44: 2084–2088, 1984

    Google Scholar 

  14. Ikeda T, Danielpoure D, Sirbasku DA: Isolation and properties of endocrine and autocrine type mammary tumor cell growth factors (estromedins). In: Bresciani F, King RJB, Lippman ME, Name M, Raynaud JP (eds) Hormones and Cancer 2; Progr Cancer Res Ther Vol. 31. Raven Press, New York, 1984, pp 171–186

    Google Scholar 

  15. Zwiebel JA, Davis MR, Kohn E, Solomon DS, Kidwell WR: Anchorage-independent growth-conferring factor production by rat mammary tumor cells. Cancer Res 42: 5117–5125, 1982

    Google Scholar 

  16. Hiragun A, Yoshida Y, Sato M, Tominaga T, Mitsui H: Isolation of two syngeneic cell lines from a rat mammary carcinoma: Growth factor production by neoplastic epithelial cells. J Natl Cancer Inst 75: 471–482, 1985

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Medicine, Division of Endocrinology, The Milton S. Hershey Medical Center, The Pennsylvania State University, P.O. Box 850, 17033-2396, Hershey, PA, USA

    Andrea Manni, Betty Badger, Carol Wright, S. Rafeeq Ahmed, Steven J. Santner & Gordon Luk

  2. The Oncology Center, The Johns Hopkins University School of Medicine, 21205, Baltimore, MD, USA

    Andrea Manni, Betty Badger, Carol Wright, S. Rafeeq Ahmed, Steven J. Santner & Gordon Luk

Authors
  1. Andrea Manni
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  2. Betty Badger
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  3. Carol Wright
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  4. S. Rafeeq Ahmed
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  5. Steven J. Santner
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  6. Gordon Luk
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Manni, A., Badger, B., Wright, C. et al. Role of polyamines in the synthesis of prolactin-regulated growth factors by experimental breast cancer in culture. Breast Cancer Res Tr 10, 191–196 (1987). https://doi.org/10.1007/BF01810582

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