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Expression and release of interleukin-1 by human glioblastoma cells in vitro and in vivo

  • Neuropathological Articles
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Summary

The present study demonstrates interleukin-1 (IL-1) production by human glioblastorna cells bothin vitro andin vivo. The presence of IL-1α and IL-1β transcripts was analyzed in 4 cell lines. IL-1α mRNA was expressed constitutively in one cell line whereas constitutive IL-1β mRNA could not be detected in any of the cell lines. IL-1α transcripts could be induced with phorbol myristate acetate (PMA) or PMA plus lipopolysaccharide (LPS) in 2 of 4 cell Unes and IL-1β mRNA in 2 of 4 cell lines. Culture fluid from these cell lines was tested for the presence of IL-1 using a specific radioimmuno-assay for either IL-1α or IL-1β. In agreement with the results on RNA, one of 4 cell lines was found to constitutively produce IL-1α but not IL-1β. After treatment with PMA and LPS, IL-1α was detected in the culture fluid from two other lines and IL-1β in the medium from three lines. That the IL-1 produced by these cell lines was biologically active was confirmed in a two step thymocyte proliferation assay. IL-1 like activity was detected in all samples that were positive in the radio-immuno-assay.

Finally, immunohistological analysis on fresh frozen tumour sections provided evidence for IL-1 production by glioblastoma cellsin vivo. Fourteen out of 28 glioblastomas were stained with an anti-IL-1α monoclonal antibody while none of them was stained with an anti-IL-1β antibody.

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Abbreviations

GFAP:

glial fibrillary acidic protein

MAb:

monoclonal antibody

IL-1:

interleukin-1

PMA:

phorbol myristate acetate

LPS:

lipopolysaccharide

RIA:

radio-immuno-assay

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Authors and Affiliations

  1. Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland

    P. Gallay

  2. Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland

    St. Carrel

  3. Neurosurgical Service, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland

    T. Gauthier, M. -F. Hamou, L. Monod & N. de Tribolet

Authors
  1. T. Gauthier
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  2. M. -F. Hamou
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  3. L. Monod
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  4. P. Gallay
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  5. St. Carrel
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  6. N. de Tribolet
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Gauthier, T., Hamou, M.F., Monod, L. et al. Expression and release of interleukin-1 by human glioblastoma cells in vitro and in vivo. Acta neurochir 121, 199–205 (1993). https://doi.org/10.1007/BF01809276

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